Method for inducing white hair model

Abstract

The invention discloses a method for inducing a white hair model. The method comprises the following steps: step 1, storing liquid and dissolving; step 2, sub-packaging the solution; step 3, injection dosage: according to the weight of the mouse, injecting a liquid substance into the body of the laboratory mouse according to the dosage of 60mg / kg; step 4, preparing a suspended working solution: taking one tube of 100 [mu] L storage solution, and adding normal saline of which the volume is 10-15 times that of the storage solution to prepare the suspended working solution; 5, paraffin unhairing, wherein paraffin unhairing is conducted on the mice of 8-9 weeks old on the 0th day; step 6, in-vivo research: according to the injection dosage of 60 mg / kg, 200 [mu] L of the working solution is continuously injected into the intraperitoneal cavity of a mouse of 8-9 weeks old and 25 g every day, and the time lasts for 2-3 weeks; and step 7, recording mouse hair growth. The Cabozantinib is used as a VEGFR2 (vascular endothelial growth factor receptor 2) inhibitor and can play a role in inducing white hair, and the white hair of a mouse grows densely in the twenty-fourth day after the mouse is injected with the Cabozantinib.

Description

technical field [0001] The invention relates to the technical field of inhibitors, in particular to a method for inducing a gray hair model. Background technique [0002] Gray hair is a clear sign of tissue degeneration during aging, and its age-related graying of hair is attributed to the dysfunction of melanocyte stem cells (McSCs). Physiologically, McSCs and their progeny can constitute the hair follicle pigment system, and the activation of McSCs is related to the hair follicle. The cycle is consistent and proliferates to replenish the McSC population, which then produces melanocytes that deposit pigment into the growing hair shaft. Studies have demonstrated that the complete loss of McSCs or their reduced function leads to the generation of pigment-deficient hair shafts, i.e. The gray hair we know is the same as our black hair. The reason why the hair of the black mouse can be black and bright depends on the melanocytes at the bottom of the hair follicles to synthesize ...

AI Technical Summary

Problems solved by technology

"However, this method cannot improve the volume of hair, and its operation is relatively cumbersome, and its effective cycle from white to black is uncertain.

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