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Preparation method of human anatomy viscera specimen

A production method and anatomical technology, applied in the field of visceral specimens, can solve problems such as tissue structure fixation, tissue structure deformation, and unfavorable anatomical research operations, and achieve the effects of simple and easy method, natural color and soft texture.

Inactive Publication Date: 2020-12-08
BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Anatomy teaching specimens have been fixed and soaked in 4% formaldehyde for many years. Formaldehyde is a chemical substance that is volatile and has many hazards to the human body. During the learning and teaching of anatomy, the stimulation of formaldehyde Taste and toxicity have caused many anatomy teachers and students to suffer greatly. The fixed and preserved tissue specimens tend to become hard and brittle, and muscle fibers are easily broken, which is not conducive to subsequent anatomical research operations.
[0004] In addition, during the preparation of visceral tissue, especially air-containing tissue specimens, the residual air inside the viscera will prevent the tissue structure from being completely fixed, resulting in the deformation of the tissue structure in the later stage, which affects the study of the normal shape of visceral tissue and observe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for preparing a human anatomical internal organ specimen, comprising the steps of:

[0046] 1) Rinse the viscera with normal saline through the artery;

[0047] 2) After the visceral artery was perfused and soaked in the pretreatment solution for 12 hours, vacuum treatment was performed at 80mm Hg, 60mm Hg, 40mmHg, and 20mm Hg, each level was 0.5h, and the viscera were pretreated twice by step 2), Before each pretreatment, fresh pretreatment liquid is replaced; wherein said pretreatment liquid includes 32wt% phenylacetaldehyde, 15wt% ethylenediaminetetraacetic acid, 2wt% 4-aminopyrrole-2-carboxylic acid, 6wt% Disodium hydrogen phosphate, 45 wt% ethanol;

[0048] 3) After the pretreatment solution was rinsed with normal saline, the internal organs were pre-cooled at 2°C for 3-5 hours, then soaked in the dehydration solution for 18 hours, and the internal organs soaked in the dehydration solution were placed In an environment at 5°C, wherein the dehydration sol...

Embodiment 2

[0053] A method for preparing a human anatomical internal organ specimen, comprising the steps of:

[0054] 1) Rinse the viscera with normal saline through the artery;

[0055] 2) After the visceral artery is perfused and soaked in the pretreatment solution for 16 hours, vacuum treatment is carried out at 80mm Hg, 60mm Hg, 40mmHg, and 20mm Hg, each level is 0.8h; the viscera are subjected to step 2) pretreatment 2 times, before each pretreatment, fresh pretreatment liquid was replaced; wherein said pretreatment liquid included 40wt% phenylacetaldehyde, 12t% ethylenediaminetetraacetic acid, 1wt% 4-aminopyrrole-2-carboxylic acid, 7wt% disodium hydrogen phosphate, 40wt% ethanol;

[0056] 3) After the pretreatment solution was rinsed with normal saline, the internal organs were pre-cooled at 2°C for 3 hours, and then soaked in the dehydration solution for 18 hours, wherein the internal organs soaked in the dehydration solution were placed at 6°C In the environment; Wherein, desc...

Embodiment 3

[0061] A method for preparing a human anatomical internal organ specimen, comprising the steps of:

[0062] 1) Rinse the viscera with normal saline through the artery;

[0063] 2) After perfusing the visceral artery and immersing it in the pretreatment solution for 24 hours, perform vacuum treatment at 80 mm Hg, 60 mm Hg, 40 mmHg, and 20 mm Hg, each level is 1 hour, and the internal organs are pretreated in step 2) 2 times, all replace fresh pretreatment liquid before each pretreatment; Wherein said pretreatment liquid comprises 45wt% phenylacetaldehyde, 12wt% ethylenediaminetetraacetic acid, 2wt% 4-aminopyrrole-2-carboxylic acid , the disodium hydrogen phosphate of 6wt%, the ethanol of 35wt%;

[0064] 3) After the pretreatment solution was rinsed with physiological saline, the internal organs were pre-cooled at 2°C for 5 hours, then soaked in the dehydration solution for 24 hours, and the internal organs soaked in the dehydration solution were placed in In an environment of...

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Abstract

The invention relates to a method for preparing a human anatomical visceral specimen. The method comprises the following steps: step one, pouring normal saline into a viscus through an artery for washing and cleaning; step two, pouring visceral and immersing the visceral artery into pretreatment liquid for 12 to 24 hours, and then carrying out vacuum treatment; step three, carrying out pre coolingon the viscus for 3 to 5h at a temperature of 2 DEG C, and immersing the cooled viscus into a dehydration solution for 16 to 24h; step four, immersing the viscus into a sealed container with preservation solution for 36 to 72h and carrying out gradient vacuum treatment; step five, removing excess material of the visceral outer layer to obtain a softened specimen; and step six, placing the softened specimen into a sealed box filled with a curing agent for 72 to 96 hours to obtain a cured specimen. The method is simple and easy. Since the pretreatment liquid and the preservation liquid are notirritating, the operator is protected from being injured; the softened visceral specimen is not easy to damage and can be used for long time; and the visceral specimen has the natural color and soft texture and has no mildew phenomenon.

Description

technical field [0001] The invention relates to a visceral specimen, in particular to a method for making a human anatomical visceral specimen. Background technique [0002] Human anatomy is a science that studies the shape and structure of the normal human body, and it belongs to the morphological category of biological science. In the field of medicine, it is an important basic course. Its task is to reveal the morphological and structural characteristics of various systems and organs of the human body, and the adjacency and affiliation relationship between various organs and structures. The course lays the foundation. [0003] Anatomy teaching specimens have been fixed and soaked in 4% formaldehyde for many years. Formaldehyde is a chemical substance that is volatile and has many hazards to the human body. During the learning and teaching of anatomy, the stimulation of formaldehyde The taste and toxicity have caused great harm to the majority of anatomy teachers and stu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00G09B23/30
CPCA01N1/00G09B23/30
Inventor 闫旭升方欣宋嵬贾建新
Owner BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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