A biologically active immune preparation for enhancing sheeppox vaccine antibody and its preparation method

Abstract

The invention relates to a biological immune preparation for strengthening a goatpox vaccine antibody and a manufacturing method of the biological immune preparation. The steps adopted in the method include that the fresh spleen of a healthy yak is selected, after fat, the envelope and the internal connective tissue are removed, the spleen is subjected to freezing preservation, the yak spleen obtained after freezing preservation is taken out and melted and is minced through a tissue stamp mill, after sterile tri-distilled water with the same weight is added, the high-speed tissue stamp mill is used for conducting homogenizing at a high speed for two times, and homogenizing is conducted 10 min each time; and prepared homogenate is frozen at the temperature of minus 20 DEG C, freezing and thawing are conducted repeatedly for five times, centrifuging is conducted after the last time of thawing, supernatant is taken and put into a dialysis bag, the supernatant is dialyzed overnight under the environment of 0 DEG C to 4 DEG C through pyrogen-free cold normal saline, dialysate outside the bag is collected and is subjected to filtration sterilization through a filtering film, and the dialysate is subjected to sterile subpackaging and is then subjected to freezing preservation. After the biological immune preparation enters an organism, the effect of the biological immune preparation can be rapidly developed and can last for several months or even longer, the immune antibody level of the vaccine is effectively improved, the biological immune preparation can be rapidly and completely absorbed by the organism without any residue, weight increasing is obvious after the biological immune preparation is injected into the organism, and a goat can be lively in spirit.

Description

[0001] 1. Technical field: [0002] The invention relates to the technical field of biological immunity, in particular to a biologically active immune preparation for enhancing sheeppox vaccine antibody and a preparation method thereof. [0003] 2. Background technology: [0004] Goatpox is one of the earliest animal diseases discovered by humans. Its discovery history can be traced back to the 2nd century AD. In China, goatpox is also called "sheep smallpox", and its pathogen is goatpox virus. The World Organization for Animal Health (OIE) defines sheeppox as an animal disease that must be declared, while my country classifies it as a first-class infectious disease. The causative agent of sheeppox belongs to the poxiridae family (poxiridae), the chordopoxvirus subfamily (chordopoxvirade), and the member of the capripoxvirus genus (capripoxvirus). The genus Capipoxvirus contains three members, namely sheep pox virus (SPV), goat pox virus (GPV), and nodular skin disease virus (...

AI Technical Summary

Problems solved by technology

However, the two viruses cross the barrier of species transmission from time to time and infect different host populations, but the infection in the natural host is more severe

Moreover, the sheep pox vaccine or the goat pox vaccine can only induce partial cross-protection against goats or sheep

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