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Bacillus quantity detection method

A detection method, bacillus technology, applied in the field of microbial detection, can solve the problem of low detection accuracy of bacillus number, achieve the effect of improving detection accuracy, surface tension and accuracy

Inactive Publication Date: 2020-04-07
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a method for detecting the number of bacillus to solve the problem in the prior art that the detection accuracy of the number of bacillus is low due to uneven distribution of spores in the diluent

Method used

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  • Bacillus quantity detection method

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Embodiment 1

[0044] This embodiment is a method for detecting the number of Bacillus subtilis in a Bacillus subtilis fermentation liquid, the detection method comprising:

[0045] Dilute the Bacillus subtilis fermentation broth by 10% with normal saline containing Tween 60 with a volume content of 3%. 7 times, the diluted Bacillus subtilis fermentation broth was spread on a plate with a coating volume of 100 μL, and then cultured at 36°C for 16 hours, and then colony counting was performed; then the number of Bacillus in the test sample was calculated according to the colony counting results.

[0046] The above-mentioned colony counting result is 208, and the number of bacillus in the sample is calculated according to the formula of bacillus number=dilution multiple×number of colonies×coating amount to be 2.08×10 10CFU / ml.

Embodiment 2

[0048] This embodiment is a method for detecting the number of Bacillus subtilis in a Bacillus subtilis fermentation liquid, the detection method comprising:

[0049] Using physiological saline containing Tween 60 to preliminarily dilute the fermentation liquid of Bacillus subtilis 10 times to obtain a diluted mother liquor, wherein the volume content of Tween 60 in the physiological saline containing Tween 60 is 3%;

[0050] Dilute the diluted mother solution with the above-mentioned physiological saline containing Tween 60 according to a 10-fold dilution factor to obtain dilutions of different dilutions, and then spread the dilutions of different dilutions on a flat plate with a coating amount of 100 μL, and then Incubate at 36°C for 16 hours, and then count the colonies;

[0051] Calculate the number of Bacillus subtilis in the Bacillus subtilis fermentation liquid according to the result of colony counting.

Embodiment 3

[0053] This embodiment is a method for detecting the number of Bacillus subtilis in a Bacillus subtilis fermentation liquid, the detection method comprising:

[0054] Using physiological saline containing Tween 60 to preliminarily dilute the fermentation liquid of Bacillus subtilis 10 times to obtain a diluted mother liquor, wherein the volume content of Tween 60 in the physiological saline containing Tween 60 is 3%;

[0055] Use a homogenizer to homogenize the diluted mother liquor for 3 minutes at a speed of 3000r / min;

[0056] The diluted mother liquor after the homogenization treatment was gradiently diluted with the above-mentioned normal saline containing Tween 60 according to a dilution factor of 10 times to obtain dilutions of different dilutions, and then the dilutions of different dilutions were plate-coated respectively. The amount of cloth was 100 μL, and then cultured at 36°C for 16 hours, and then counted colonies;

[0057] Calculate the number of Bacillus subti...

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Abstract

The embodiment of the invention discloses a bacillus quantity detection method, and relates to the technical field of microbiological detection. The method comprises the following steps: diluting a bacillus-containing detection sample by adopting normal saline containing an emulsifier; coating a flat plate with the diluent, culturing to obtain a bacillus colony, and calculating the number of bacillus in the detection sample according to a statistical result of the bacillus colony. According to the detection method disclosed by the invention, the normal saline containing the emulsifier is usedfor diluting the detection sample; the emulsifier can improve the surface tension of the bacillus in different states in the normal saline, so that dispersion of the bacillus in different states in the normal saline is better promoted, the bacillus can be fully and uniformly distributed in the normal saline, and the accuracy of bacillus quantity detection is further improved.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of microorganism detection, and in particular to a method for detecting the number of bacillus. Background technique [0002] Bacillus (Bacillus) is a bacillus or coccus capable of forming spores (endospores), including Bacillus, Lactobacillus, Clostridium, Desulfuricentericus and Bacillus sarcinia, etc., and widely exists in soil, water, air and animal intestines. Bacillus can secrete a variety of enzymes and antibiotics that can inhibit the growth of animal pathogens, and can directly use nitrate and nitrite in water to purify water quality. It plays an important role in environmental purification, aquatic water purification, and animal feed. Applications. [0003] At present, there are more and more products containing bacillus in the market, and the number of viable bacillus in the product is an important indicator to measure the quality of the product. Therefore, in order to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12R1/07
CPCC12Q1/06
Inventor 孙长坡杜稳刘虎军赵一凡常晓娇
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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