Kit for diagnosis and assessment of breast cancer, and detection and applications of methylated HOXA4/DPP6 gene

A HOXA4 and kit technology, applied in the field of biomedicine, can solve problems such as lack of research, and achieve the effects of good specificity, high accuracy and high precision.

Active Publication Date: 2018-05-04
SHENZHEN CITY BAOAN DISTRICT MATERNAL & CHILD HEALTH HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology, relevant research is still very scarce, and more research is urgently needed to reveal the methylated genes related to breast cancer and clarify the relationship between the methylated sites and breast cancer

Method used

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  • Kit for diagnosis and assessment of breast cancer, and detection and applications of methylated HOXA4/DPP6 gene
  • Kit for diagnosis and assessment of breast cancer, and detection and applications of methylated HOXA4/DPP6 gene
  • Kit for diagnosis and assessment of breast cancer, and detection and applications of methylated HOXA4/DPP6 gene

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Embodiment 1

[0029] A kind of application of methylated HOXA4 / DPP6 gene, the accession numbers of described HOXA4 gene and DPP6 gene in the DNA sequence database are as shown in Table 1; The methylation of HOXA4 / DPP6 gene is CpG dinucleotide 5' The -terminal cytosine is converted to 5'-methylcytosine. Through the research of the present inventors, it is found that the methylation of HOXA4 / DPP6 gene in breast cancer tumors is greatly improved, wherein the methylation rate of HOXA4 gene in tumor tissue reaches 80.58%, and the methylation rate of DPP6 gene reaches 46.9%, which is higher than that in normal tissue. The methylation of HOXA4 / DPP6 gene was significantly increased. Therefore, the methylated HOXA4 / DPP6 gene can be used in the assessment, diagnosis, detection or screening of drugs in breast cancer.

[0030] Table 1: Accession numbers of the HOXA4 / DPP6 gene

[0031] gene symbol

Embodiment 2

[0033] A kit for diagnosing and evaluating breast cancer, said kit is a kit using methylated HOXA4 gene and / or methylated DPP6 gene as a biomarker, preferably simultaneously methylated HOXA4 / DPP6 Genes as biomarkers.

[0034] The kit includes specific primers designed for the biomarkers, and also includes TRIzol reagent (purchase manufacturer: Life Tech Inc, USA), 0.1M sodium citrate containing 10% ethanol, CT conversion agent (from EZ DNAMethylation TM Kit (ZYMO RESEARCH, Cat. No. D5002)), M-binding buffer, M-washing buffer, M-desulphonation buffer, bisulfite solution, Zymo Taq premix.

[0035] The above primers are to amplify the CpG of the 5'UTR of the methylated HOXA4 / DPP6 gene or the CpG of the exon 1 region to detect the methylation of the CpG, preferably including the amplification of the unmethylated HOXA4 / DPP6 gene Primers for the CpG of the 5'UTR or the CpG of the exon 1 region.

[0036] Specifically, the primers include a methylated HOXA4 gene primer and a methyla...

Embodiment 3

[0039] A kind of detection of methylation HOXA4 / DPP6 gene, described detection method is to take methylation HOXA4 / DPP6 gene as target, adopts illumina methylation chip (article number: WG-314-1003), and according to embodiment 2 The kit detects the degree of methylation of the HOXA4 / DPP6 gene. The specific method is referred to embodiment 4.

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Abstract

The invention relates to a kit for diagnosis and assessment of HOXA4 / DPP6 gene, and detection and applications of a methylated HOXA4 / DPP6 gene, and in particular to an HOXA4 / DPP6 gene, methylation detection of the HOXA4 / DPP6 gene, applications of the HOXA4 / DPP6 gene in preparing breast cancer diagnosis, detection and screening drugs and a kit for the applications. According to the invention, basedupon researches, it is discovered that a methylation degree of the HOXA4 / DPP6 gene in tumor tissues of a patient with the breast cancer is obviously higher than that of normal tissues beside the tumor tissues, and moreover, a methylation level is closely related to tumor progression; the invention provides a novel breast cancer diagnosis and treatment target and provides a kit corresponding to the breast cancer diagnosis and treatment target; therefore, a novel method is provided for detecting the progression of the breast cancer and for population screening and breast cancer diagnosis, and an important clinical application value is achieved.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to the methylation of HOXA4 / DPP6 gene and its application, and more specifically to a kit for diagnosing and evaluating breast cancer, HOXA4 / DPP6 gene, methylation of HOXA4 / DPP6 gene Detection and its application in breast cancer. Background technique [0002] According to GLOBOCAN, breast cancer is the most common cancer among Chinese women, with an age-standardized rate (ASR) of 21.6 cases per 100,000 people. According to the data of "Cancer Statistics in China, 2015" published by Academician He Jie, breast cancer ranks first in the incidence of female tumors in my country, with 272,000 new cases in 2015, and 190,000 new female breast cancers in urban areas. According to the China National Cancer Registry Center, the rate of ASR in urban areas (34.3 cases / 100,000 women) is twice that of rural areas (17.0 cases / 100,000 women). The coastal cities with developed socio-e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158
Inventor 李少英
Owner SHENZHEN CITY BAOAN DISTRICT MATERNAL & CHILD HEALTH HOSPITAL
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