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Related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance

A technology of Mycobacterium tuberculosis and complex flora, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of high cost, long cycle, narrow detection range, etc., and achieve accurate drug resistance detection Effect

Inactive Publication Date: 2021-08-13
上海康黎诊断技术有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to provide a primer set and a kit that utilize time-of-flight mass spectrometry to realize the identification of Mycobacterium tuberculosis complex flora and the detection of drug resistance, so as to solve the problem of low sensitivity and cycle time of detection methods for Mycobacterium tuberculosis in the prior art. Shortcomings of length, high cost, narrow detection range, and the inability to realize the identification of Mycobacterium tuberculosis complex flora and the detection of drug resistance at the same time

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  • Related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance
  • Related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance
  • Related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance

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[0410] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention but not to limit the scope of the present invention.

[0411] 1.1 Inspection principle

[0412] The detection method adopted in the present invention is nucleic acid flight mass spectrometry. First, the target sequence is amplified by PCR, and then the SNP sequence-specific extension primer is added to extend 1 base at the SNP site. After the prepared sample analyte is co-crystallized with the chip matrix, it is excited by a strong laser in the vacuum tube of the mass spectrometer, and the nucleic acid molecules are desorbed into single-charged ions. The ion flight time in the electric field is inversely proportional to the ion mass. By detecting the nucleic acid molecules in the vacuum tube The precise molecular weight of the sample analyte is obtained by time-of-flight, s...

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Abstract

The invention provides a related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance. According to the related gene, method, primer group and kit, not only can the mycobacterium tuberculosis complex flora be identified, but also 16 common anti-tuberculosis first-line and second-line drug resistance gene mutations of the mycobacterium tuberculosis complex flora can be distinguished and detected at high resolution, and rapid, simultaneous and accurate drug resistance detection is achieved. The related gene, method, primer group and kit can be used for clinical treatment reference and epidemiological research.

Description

technical field [0001] The invention relates to the detection of pathogenic microorganisms, and more specifically relates to a related gene, method, primer set and kit for identification of Mycobacterium tuberculosis complex flora and drug resistance detection. Background technique [0002] Mycobacterium tuberculosis is the most important factor causing various tuberculosis (mainly pulmonary tuberculosis). Although epidemiological data show that tuberculosis is prevalent in a large area, the effective rate of tuberculosis treatment is only 55%, and drug resistance is an important reason for this situation. In 2017, 5.6% of TB patients in my country were multidrug-resistant TB (MDR-TB), and among these patients, an estimated 8.5% were extensively drug-resistant TB (XDR-TB). Therefore, the identification of Mycobacterium tuberculosis and its drug susceptibility testing are of great public health significance. [0003] Due to the small genome differences between each other, f...

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6858C12Q1/04C12N15/31C12N15/11
CPCC12Q1/689C12Q1/6858C12Q2600/106C12Q2600/156C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 何熲石忆湘黄成琛谢海波杨军魏宁王保曼张辉
Owner 上海康黎诊断技术有限公司
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