Method for testing oxidative stress biomarkers in blood plasma with low coefficient of variation
A technology of biomarkers and coefficient of variation, applied in the field of oxidative stress biomarkers in testing plasma with low coefficient of variation, can solve the problem of high coefficient of variation, and achieve the effect of improving reliability, reliable measurement method, and increasing possibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0015] The divided plasma was extracted with ethanol / ether (v / v 3:1), and the extraction ratio was plasma:extraction solution=1:20. Example 1 uses 62 μL of plasma and 1238 μL of extract for each sample for extraction. Add the extract to the plasma and shake it well to make the plasma fully react with the extract. Immediately thereafter, centrifuge at 3000 rpm for 10 min at 4°C to precipitate the flocs formed by the reaction. After aspirating the supernatant, centrifuge the supernatant again under the same conditions for 5 min, in order to centrifuge the precipitate sufficiently so as not to affect the measured fluorescence intensity value. The supernatant was loaded into a CORNING 96-well black fluorescent plate (FLATBOTTOM, REF3925), 300 μL was added to each well, and the reading height was 7.00 mm. Fluorescence intensity of the liquid at excitation wavelength / emission wavelength: 320 / 420nm, 360 / 420nm, 400 / 475nm (as shown in Table 1 below).
[0016] In order to facilitate ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com