A monoclonal antibody for H1N1 swine influenza A virus hemagglutinin protein and a double-antibody sandwich ELISA kit

Abstract

A monoclonal antibody for H1N1 swine influenza A virus hemagglutinin protein and a double-antibody sandwich ELISA kit are disclosed. The kit comprises a solid phase vector coated with the monoclonal antibody, a rabbit polyclonal antibody labeled by horseradish peroxidase, an H1N1 hemagglutinin protein standard substance, a sample diluting liquid, a washing liquid, a substrate coloring liquid and a reaction terminating liquid. The kit is good in sensitivity, capable of performing quantitative detection for the swine influenza virus hemagglutinin protein, and specifically recognizing main prevalent strains of A(H1N1) influenza outbreak in 2009, and free of cross reactions with other strains of the H1N1 influenza viruses and other influenza virus subtypes. The kit is simple to operate, can be used for supporting fundamental research of the H1N1 swine influenza virus, and has important significance for performing epidemiologic study on the H1N1 influenza viruses in different zones and different years.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to an anti-H1N1 swine influenza virus hemagglutinin protein monoclonal antibody and a double-antibody sandwich ELISA quantitative detection kit. Background technique [0002] Influenza A virus H1N1 subtype, also known as H1N1 virus, is an RNA virus belonging to the Orthomyxoviridae family, a type of influenza A virus, and one of the most commonly infected influenza viruses in humans. Some strains of H1N1 can spread between humans, including the 1918 influenza pandemic, and others can spread between birds and pigs. H1N1 is the abbreviation of the names of two important target proteins of the virus, where "H" refers to hemagglutinin (Hemagglutinin), also known as hemagglutinin protein, and "N" refers to neuraminidase (Neuraminidase). Thus, H1N1 is a subtype of influenza virus that has the "hemagglutinin type 1 and neuraminidase type 1" viral proteins. [0003] Influenza pand...

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Problems solved by technology

[0004] Although the fatality rate of influenza A (H1N1) is not very high, the influenza virus often has strong variability. Due to the high immune pressure of H1N1, the amino acid changes of the eight gene segments continue to accumulate, and the antigenic variation is obvious. Once some mutations lead to The obvious increase in pathogenicity will have an immeasurable impact on human life safety and social stability. Therefore, the long-term effective monitoring and research on H1N1 swine influenza virus and other influenza viruses has increasingly significant public health significance

The currently published research techniques for H1N1 swine influenza virus hemagglutinin antigen mainly rely on chicken embryo isolation, PCR, etc. Virus isolation takes a long time, and PCR requires professional equipment and technicians, which are not suitable for popularization. Some ELISAs for qualitative swine influenza detection kits, it is impossible to carry out accurate quantitative detection of viral antigenic proteins, and it is often difficult to accurately distinguish between different strains of H1N1 influenza virus and other influenza virus subtypes, which is not conducive to supporting basic and epidemiological research on influenza viruses, and there is an urgent need for a A quantitative detection reagent with simple operation, good sensitivity and high specificity

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