Kit for detecting ST251-type virulent aeromonas hydrophila and application

A technology based on Aeromonas hydrophila and ST251, which is applied in the direction of microorganism-based methods, measurement/inspection of microorganisms, microorganisms, etc., can solve the problems of indistinguishability, instability, laborious accuracy, etc., and achieve strong pertinence and high accuracy The effect of specificity and high sensitivity
CN105969907AActive Publication Date: 2016-09-28INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF AQUATIC LIFE ACAD SINICA
Publication Date
2016-09-28

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Abstract

The invention discloses a PCR kit for detecting ST251-type aeromonas hydrophila and application. The kit comprises a PCR Premix, a primer pair, a standard positive DNA template, a standard negative DNA template and sterilization double distilled water. An upstream primer sequence is 5'-GCGGCGTAAGCGGATTTGTAGC-3', a downstream primer sequence is 5'-CCGGTCGGATTGGTCAGGTTCAT-3', the annealing temperature is 63 DEG C, a correct amplification product is 826bp in size, the standard positive DNA template is genomic DNA extract of an ST251-type aeromonas hydrophila XS91-4-1 strain, and the DNA concentration is 500 nanograms per microliter; the negative DNA template is genomic extractive of an aeromonas veronii IB340 strain, and the DNA concentration is 500 nanograms per microliter. The kit is applicable to all kinds of gene amplification instruments, high in detection specificity and sensitivity, quick, accurate and good in stability. The kit can be used for quickly diagnosing freshwater fish outbreak diseases and blood poisoning caused by aeromonas hydrophila of other fishes and quickly detecting and identifying bacteria and then is widely applied to disease prediction and forecast and epidemiologic studies.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically relates to a kit for rapidly detecting ST251 pathogenic Aeromonas hydrophila, and also relates to the use of a kit for detecting ST251 pathogenic Aeromonas hydrophila, It can be used for rapid differential diagnosis and epidemiological research of outbreaks of freshwater farmed fish and sepsis caused by Aeromonas hydrophila in other freshwater fish, and can also provide technical support for related basic research. Background technique

[0002] Aeromonas (aeromonads) is the most common pathogenic bacteria in freshwater cultured fish in my country, which can attack almost all kinds of aquaculture animals. They exist in various ecological niches in aquaculture systems and are one of the main resident bacteria in the gut of healthy aquatic animals. Classification belongs to γ-proteobacteria, Aeromonas family, and Aeromonas genus. There are at least 25 recognized species, and new s...

Claims

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