Large brill rhabdo virus toxic strain and its preparation method and application
A rhabdovirus and turbot technology, which is applied in the fields of aquatic animal disease pathogens and isolation, in vitro culture and identification, can solve the problems of difficult control of fish infection experimental conditions, poor repeatability and high cost
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[0020] All utensils, media, and reagents used for cell culture and virus amplification are required to be sterilized by autoclaving or filter sterilization in advance to keep them sterile. The preparation methods of some commonly used reagents are as follows:
[0021] Phosphate buffered saline (PBS, Ca-free ++ , Mg ++ ):
[0022] NaCl 8.00g
[0023] KCl0.20g
[0024] Na 2 HPO 4 2.31g
[0025] K H 2 PO 4 0.31g
[0026] Dissolve in 500ml of double distilled water, add 5ml of 0.4% phenol red solution, stir evenly, add double distilled water to 1000ml. 10 lbs sterilized for 15 minutes for use.
[0027] Double Antibody (Penicillin, Streptomycin) Solution
[0028] will be 10 6 Unit Penicillin G and 10 6 μg streptomycin was dissolved in 100ml sterilized three-distilled water to make 10 per ml penicillin 4 unit, streptomycin 10 4 μg.
[0029] TC119 medium
[0030] Add the purchased TC199 medium powder according to the amount required in the instructions, dilute ...
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