A kind of cultivation method of ammonia oxidizing bacteria

Abstract

The invention provides a culturing method for ammonia oxidizing bacteria. The culturing method comprises the following steps: inoculating ammonia oxidizing bacteria liquid into a fermentation tank; carrying out fermentation culture for 5-7 days; collecting fermentation liquid, so as to obtain the ammonia oxidizing bacteria, wherein the inoculation amount is 5%; the culturing conditions refer to that a pH (Potential of Hydrogen) value is controlled to be 7.5-8.0 by adding a base liquid, a temperature is 25-30 DEG C, a ventilation amount is 1.5-2.0M<3> / h, a tank pressure is 0.05-0.1MPa, and a rotation speed is 180-200r / min; a culture medium is a nitrobacteria culture medium. The culturing method uses a fermentation tank yeast propagation method, so that infectious microbes are difficult to pollute; various parameters, such as the temperature, the pH value, dissolved oxygen and a stirring rotation speed and the like, can be stably controlled; compared with the traditional opened type yeast propagation method, the culturing method for the ammonia oxidizing bacteria can be used for more economically and effectively preparing an ammonia oxidizing bacteria agent, so that the agent can be rapidly and effectively propagated; the culturing method for the ammonia oxidizing bacteria is suitable for large-scale industrial production and has a wide application prospect in a sewage treatment field.

Description

Technical field

[0001] The invention relates to a method for culturing bacteria, in particular to a method for culturing ammonia oxidizing bacteria. Background technique

[0002] Ammonia nitrogen is one of the most important pollutants in water bodies: in an alkaline environment, ammonia nitrogen is toxic to aquatic organisms; ammonia nitrogen is oxidized into nitrate nitrogen and nitrite, which will cause blooms; nitrate and nitrite have high concentrations Drinking water may cause health hazards to the human body. With the development of industrial and agricultural production, the pollution of ammonia nitrogen has become more and more serious, which has become one of the urgent problems in the field of sewage treatment and one of the environmental indicators monitored by the state.

[0003] The traditional physical and chemical methods of removing ammonia nitrogen include air stripping, resin-based or zeolite-based selective ion exchange, chemical precipitation, chemical neutral...

Examples

Embodiment 1

[0021] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=7.5, temperature=25℃, and aeration volume of 1.5M 3 / h, the tank pressure is 0.05MPa, and the speed is 180r / min. The fermentation culture is carried out for 5 days, the fermentation broth is collected, and the number of ammonia oxidizing bacteria in the fermentation broth is detected by the plate counting method to be 2.54×10 9 CFU / ml.

Embodiment 2

[0023] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=8.0, temperature=30℃, and aeration rate of 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.36×10 10 CFU / ml.

Embodiment 3

[0025] The volume of the fermenter used is 200L, 120L of nitrifying bacteria culture medium is added, and the seed liquid is 6L. The control parameters are: pH=8.0, temperature=30℃, and ventilation volume is 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.09×10 10 CFU / ml.

Technical field

[0001] The invention relates to a method for culturing bacteria, in particular to a method for culturing ammonia oxidizing bacteria. Background technique

[0002] Ammonia nitrogen is one of the most important pollutants in water bodies: in an alkaline environment, ammonia nitrogen is toxic to aquatic organisms; ammonia nitrogen is oxidized into nitrate nitrogen and nitrite, which will cause blooms; nitrate and nitrite have high concentrations Drinking water may cause health hazards to the human body. With the development of industrial and agricultural production, the pollution of ammonia nitrogen has become more and more serious, which has become one of the urgent problems in the field of sewage treatment and one of the environmental indicators monitored by the state.

[0003] The traditional physical and chemical methods of removing ammonia nitrogen include air stripping, resin-based or zeolite-based selective ion exchange, chemical precipitation, chemical neutral...

Examples

Embodiment 1

[0021] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=7.5, temperature=25℃, and aeration volume of 1.5M 3 / h, the tank pressure is 0.05MPa, and the speed is 180r / min. The fermentation culture is carried out for 5 days, the fermentation broth is collected, and the number of ammonia oxidizing bacteria in the fermentation broth is detected by the plate counting method to be 2.54×10 9 CFU / ml.

Embodiment 2

[0023] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=8.0, temperature=30℃, and aeration rate of 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.36×10 10 CFU / ml.

Embodiment 3

[0025] The volume of the fermenter used is 200L, 120L of nitrifying bacteria culture medium is added, and the seed liquid is 6L. The control parameters are: pH=8.0, temperature=30℃, and ventilation volume is 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.09×10 10 CFU / ml.

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