A kind of cultivation method of ammonia oxidizing bacteria
A technology of ammonia oxidizing bacteria and a culturing method, applied in the field of culturing ammonia oxidizing bacteria, can solve the problems of slow start-up, long time consumption, unstable conditions and the like of a reactor
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Embodiment 1
[0021] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=7.5, temperature=25℃, and aeration volume of 1.5M 3 / h, the tank pressure is 0.05MPa, and the speed is 180r / min. The fermentation culture is carried out for 5 days, the fermentation broth is collected, and the number of ammonia oxidizing bacteria in the fermentation broth is detected by the plate counting method to be 2.54×10 9 CFU / ml.
Embodiment 2
[0023] The volume of the fermenter used is 30L, 19L of nitrifying bacteria culture medium is added, and 1L of seed liquid is added. The control parameters are: the fermentation tank is automatically supplemented with 10% sodium bicarbonate solution to control pH=8.0, temperature=30℃, and aeration rate of 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.36×10 10 CFU / ml.
Embodiment 3
[0025] The volume of the fermenter used is 200L, 120L of nitrifying bacteria culture medium is added, and the seed liquid is 6L. The control parameters are: pH=8.0, temperature=30℃, and ventilation volume is 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation was carried out for 7 days, the fermentation broth was collected, and the number of ammonia oxidizing bacteria in the fermentation broth was detected by the plate counting method to be 1.09×10 10 CFU / ml.
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