Application of PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products

A rhabdovirus and inhibitor technology, which is applied in the application field of preparing anti-fish rhabdovirus products, can solve problems such as endangering human safety and aquaculture environment residues, etc.

Active Publication Date: 2021-04-02
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although inactivated vaccines and attenuated vaccines have played a good role in the prevention of IHNV, VHSV and SVCV, they are easy to cause residues in the breeding environment during their use, and may lead to pathogen resistance and virus mutations. form
In contrast, nucleic acid vaccines are relatively safe, but the currently used nucleic acid vaccines use CMV as the promoter to express the target antigen gene. Since the CMV promoter is the promoter of human pathogens, nucleic acid vaccines have the potential to endanger human safety. possible, severely limiting its application

Method used

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  • Application of PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products
  • Application of PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products
  • Application of PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products

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Embodiment 1

[0034] Example 1, the application of PARP inhibitor Rucaparib in anti-fish rhabdovirus

[0035] 1. Experimental materials

[0036] 1.1 Cells and viruses

[0037] EPC cell ATCCCRL-2872; Infectious hematopoietic organ necrosis virus Sn1203 strain (referred to as IHNV-Sn1203 strain) (Genbank accession number: KC660147.1) (reference "Zhao Jingzhuang, Xu Liming, Ren Guangming, et al. Infectious hematopoietic organ necrosis virus Sn1203 Strain whole genome sequence and phylogenetic analysis[J]. Journal of Fisheries Science, 2020, v.33(02):4-12."); Viral hemorrhagic sepsis virus strain (VHSV-1387 for short) (ATCC VR1387); Carp Spring Viraemia Virus Shlj1 Strain (SVCV-Shlj1 for short) (Reference "Ji Feng. Prediction of Glycoprotein Spatial Structure and B Cell Epitope of Carp Spring Viremia Virus Shlj1 Strain [J]. Journal of Dalian Ocean University , 2017, 32(4):440-446.”).

[0038] 1.2 Reagents

[0039] Cell culture medium MEM (C11095500BT), trypsin (25200072), fetal bovine serum (...

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Abstract

The invention discloses application of a PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products. According to the application, a plurality of PARP inhibitors are screened, andthe result shows that the PARP inhibitor Rucaparib can inhibit mRNA replication of viruses IHNV, VHSV and SVCV in cells and reduce the titer (TCID50) of the viruses IHNV, VHSV and SVCV; the inhibitioneffect of Rucaparib on IHNV, VHSV and SVCV is in dose-dependent relation. The PARP inhibitor Rucaparib is expected to become a novel drug for resisting fish rhabdovirus.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to the application of the PARP inhibitor Rucaparib in the preparation of anti-fish rhabdovirus products. Background technique [0002] Since 2010, the output of the world's aquaculture industry has grown rapidly, but the spread of infectious diseases has seriously affected the healthy and sustainable development of aquaculture. The research and application of therapeutic and preventive drugs have become an urgent need for the aquaculture industry. [0003] Infectious hematopoietic necrosis (IHN) is one of the main diseases endangering salmon and trout farming in the world. It is a serious threat caused by infectious hematopoietic necrosis virus (IHNV) Acute infectious diseases of trout can usually cause 80%-100% mortality depending on the fish species, fish age, virus strains and feeding environment. [0004] Viral haemorrhagic septicaemia (VHS) is an infectious disease caused by viral h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/55A61P31/14
CPCA61K31/55A61P31/14
Inventor 赵景壮卢彤岩徐黎明任广明邵轶智
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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