Application of parp inhibitor pj34 in the preparation of anti-fish rhabdovirus products

A technology of rhabdoviruses and inhibitors, applied in the field of aquaculture, can solve problems such as endangering human safety and residues in the aquaculture environment

Active Publication Date: 2022-03-01
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although inactivated vaccines and attenuated vaccines have played a good role in the prevention of IHNV, VHSV and SVCV, they are easy to cause residues in the breeding environment during their use, and may lead to pathogen resistance and virus mutations. form
In contrast, nucleic acid vaccines are relatively safe, but the currently used nucleic acid vaccines use CMV as the promoter to express the target antigen gene. Since the CMV promoter is derived from human pathogens, nucleic acid vaccines are dangerous to humans. security possibilities, which severely limits its application

Method used

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  • Application of parp inhibitor pj34 in the preparation of anti-fish rhabdovirus products
  • Application of parp inhibitor pj34 in the preparation of anti-fish rhabdovirus products
  • Application of parp inhibitor pj34 in the preparation of anti-fish rhabdovirus products

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Embodiment 1

[0033] Example 1, the application of PARP inhibitor PJ34 in anti-fish rhabdovirus

[0034] 1. Experimental materials

[0035] 1.1 Cells and viruses

[0036]EPC cell ATCCCRL-2872; Infectious hematopoietic organ necrosis virus Sn1203 strain (referred to as IHNV-Sn1203 strain) (Genbank accession number: KC660147.1) (reference "Zhao Jingzhuang, Xu Liming, Ren Guangming, et al. Infectious hematopoietic organ necrosis virus Sn1203 Strain whole genome sequence and phylogenetic analysis[J]. Journal of Fisheries Science, 2020, v.33(02):4-12."); Viral hemorrhagic sepsis virus strain (VHSV-1387 for short) (ATCC VR1387); Carp Spring Viraemia Virus Shlj1 Strain (SVCV-Shlj1 for short) (Reference "Ji Feng. Prediction of Glycoprotein Spatial Structure and B Cell Epitope of Carp Spring Viremia Virus Shlj1 Strain [J]. Journal of Dalian Ocean University , 2017, 32(4):440-446.”).

[0037] 1.2 Reagents

[0038] Cell culture medium MEM (C11095500BT), trypsin (25200072), fetal bovine serum (1009...

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Abstract

The invention discloses the application of PARP inhibitor PJ34 in the preparation of anti-fish rhabdovirus products. The present invention provides PARP inhibitor PJ34 or derivatives thereof or pharmaceutically acceptable salts thereof or substances with PARP inhibitors PJ34 or derivatives thereof or pharmaceutically acceptable salts thereof as active ingredients in the preparation of anti-fish bomb Applications in virus products. The present invention has screened multiple PARP inhibitors, and found that PARP inhibitor PJ34 can suppress the mRNA replication of IHNV, VHSV and SVCV virus in cells, and reduce the titer of IHNV, VHSV and SVCV virus simultaneously (TCID 50 ); and the inhibitory effect of PJ34 on IHNV, VHSV and SVCV showed a dose-dependent relationship. PARP inhibitor PJ34 is expected to be a new drug against rhabdovirus in fish.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to the application of PARP inhibitor PJ34 in the preparation of anti-fish rhabdovirus products. Background technique [0002] Since 2010, the output of the world's aquaculture industry has grown rapidly. However, the spread of infectious diseases has seriously affected the healthy and sustainable development of aquaculture. The research and application of preventive and therapeutic drugs have become an urgent need for the aquaculture industry. [0003] Infectious hematopoietic necrosis (IHN) is one of the major diseases endangering salmon and trout farming in the world. It is caused by infectious hematopoietic necrosis virus (IHNV), which is a serious threat to 1. Acute infectious diseases of trout can usually cause 80%-100% mortality depending on the fish species, fish age, virus strains and breeding environment. [0004] Viral haemorrhagic septicaemia (VHS) is an infectious disease mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/473A61P31/14
CPCA61K31/473A61P31/14
Inventor 赵景壮徐黎明卢彤岩任广明邵轶智
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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