Mandarin fish rhabdo virus toxic strain, its preparation method and application

A technology of rhabdovirus and mandarin fish, which is applied to the separation, purification and preparation of aquatic animal virus pathogens in Ming Dynasty, can solve the problem of limited disease effect and achieve the effect of accurate spot-picking and purification

Inactive Publication Date: 2004-06-02
INST OF AQUATIC LIFE ACAD SINICA
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This result also suggests that although a single vaccine is effective in the prevention of diseases caused by

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  • Mandarin fish rhabdo virus toxic strain, its preparation method and application
  • Mandarin fish rhabdo virus toxic strain, its preparation method and application
  • Mandarin fish rhabdo virus toxic strain, its preparation method and application

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Embodiment Construction

[0020] The mandarin fishes with typical symptoms were collected in the endemic area where the mandarin fish epidemic occurred. Ischemia and whitish. According to observations, once these symptoms appear, the mandarin fish in the fish pond will die in large numbers within 3-7 days. Take out the visceral tissues (including liver, spleen, kidney, etc.) of the diseased fish, cut them into pieces, add 1-2 times the volume of phosphate buffer solution (PBS) for homogenization, add double antibodies, and freeze overnight at -20°C. Take it out the next day and wait for it to melt, then centrifuge at a speed of 1000-2000 rpm for 10 minutes to obtain SCRV 0208 Crude virus extract. Prepared SCRV 0208 Crude virus extract was filtered and cultured in grass carp fin cell GCF 0208 Proliferate in. in SCRV 0208 The ultra-thin section of the virus crude extract infected cells, observed by electron microscope, shows that there are spherical viruses and rhabdoviruses in the cells ( figure ...

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Abstract

The Siniperca chuatsi rhabdovirus, SCRV0208, with preservation number of CCTCC No. V202008, is prepared through culture of GCF0208 cell in TC199 culture medium with ox serum to grow single layer of cell, inoculation with virus liquid, sampling in different stages, slicing, electronic microscope observation to determine the dot picking time, picking dot and inoculating to the single layer of cell for enlarged culture, and centrifugating to obtain pure virus. The said process is simple and practical, can separate rhabdovirus from accompanied virus to obtain high purity virus antigen for application in cell engineering vaccine and genetic engineering vaccine.

Description

technical field [0001] The present invention relates to the isolation, purification and preparation technology of aquatic animal virus pathogens, in particular to the in vitro propagation and detection technology of freshwater famous and high-quality fish viruses, the preparation technology of pure virus antigens, and the material sources for the development of virus cell engineering vaccines and genetic engineering vaccines. More specifically, it relates to a mandarin fish rhabdovirus strain, a method for preparing the mandarin fish rhabdovirus strain, and the detection, identification, cell engineering vaccine and genetic engineering of the mandarin fish rhabdovirus strain in freshwater fish virus pathogens. Use in vaccine development. Background technique [0002] Mandarin fish (Siniperca chuatsi, mandarin fish) is a carnivorous fish. Particularly favored freshwater farmed fish species. There is a large domestic and foreign demand for mandarin fi...

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Application Information

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IPC IPC(8): A61K39/12C12N7/01C12Q1/70
Inventor 张奇亚陶建军李正秋桂建芳
Owner INST OF AQUATIC LIFE ACAD SINICA
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