44results about How to "Effective secretion" patented technology

The invention discloses a method for preparing soluble yeast glucan. The method comprises the following steps: mixing the powdered yeast glucan with grinding balls according to the mass ratio of 1:(5-20), treating by using planetary ball milling for 5-30min at the power of 900-1200W, mixing the prepared yeast glucan with water according to the mass ratio of 1:(10-25) to dissolve the yeast glucan, centrifuging to remove insoluble substances to obtain the yeast glucan solvent. The method has the characteristics of being simple, rapid, efficient and clean, and the prepared yeast glucan has favorable water solubility and excellent immunological competence.

The invention discloses a mutant Pul 324 of a pullulanibacillus naganoensis pullulanase and a use thereof. Based on a modern enzyme engineering technology, an amino acid sequence of a pullulanibacillus naganoensis pullulanase is subjected to molecular modification. Through a PCR method, front 108 amino acid residues of a raw enzyme are deleted so that the mutant Pul 324 which can realize effective secretory expression in escherichia coli hosts is obtained. The mutant Pul 324 is inserted into pET-22b(+) so that a recombinant vector is constructed. The recombinant vector is introduced into an escherichia coli host for secretory expression of the mutant Pul 324. The mutant Pul 324 gene is smaller than a wild type gene and is conducive to plasmid stabilization. Compared with a raw enzyme, themutant Pul 324 can realize effective secretory expression in escherichia coli hosts, realize a fermentation supernatant enzyme activity improved by 24 times and realize an endoenzyme activity improved by 40%. The mutant Pul 324 can improve a pullulanase secretory expression level of a host. An expression product can improve starch hydrolysis efficiency.

The invention relates to the technical field of waistcoats, in particular to a magnetic waistcoat. The magnetic waistcoat comprises a waistcoat main body. A magnetic care cushion block capable of abutting against the body parts is arranged on the inner side of the waistcoat main body and comprises a basis cushion block body. Massage reinforcing ribs arranged at intervals are arranged on one side, close to the body parts, of the basis cushion block body, and the massage reinforcing ribs are in strip shapes and extend in the direction from the head to the feet; strip-shaped grooves with groove openings facing the body parts and extending in the direction from the head to the feed are located at the portions, between the adjacent massage reinforcing ribs, of the basis cushion block body, and at least a line of magnetic particles arranged at intervals in the direction from the head to the feet are arranged in the strip-shaped grooves. The magnetic waistcoat is stable in structure, comfortable in wearing and good in magnetic care effect.

An eucaryotic expression of E.coli heat-labile enterotoxin B subunit, LT-B is prepared by defining nucleic acid endoenzyme site, connecting LT-B nucleic acid sequence with pPICZ alpha A to obtain recombinant expression carrier, inducing recombinant expression carrier into Pichiapastoris X-33 or KM71, and fermentation expressing to obtain target protein LT-B. It has high economical value.

A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant.Thus, it becomes possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.

The invention discloses a method for preparing soluble yeast glucan. After mixing powdered yeast glucan and grinding balls according to the mass ratio of 1:5~20, the planetary ball mill is used for processing under the condition of power 900~1200W After 5-30 minutes, the obtained yeast glucan was mixed with water at a mass ratio of 1:10-25 to dissolve the yeast glucan, and the insoluble matter was removed by centrifugation to obtain a yeast glucan solution. The method of the invention has the characteristics of simplicity, rapidity, high efficiency and cleanness, and the prepared yeast glucan has better water solubility and excellent immune activity.

The invention belongs to the technical field of biomedicine and relates to codon-optimized severe fever with thrombocytopenia syndrome virus nucleoprotein gene and nucleic acid vaccine thereof. The codon-optimized gene sequence takes into account the codon usage preference of mammalian cells, and the sequence is shown in SEQ ID NO.2. The involved severe fever with thrombocytopenia syndrome virus nucleoprotein nucleic acid vaccine is composed of codon-optimized severe fever with thrombocytopenia syndrome virus nucleoprotein gene sequence and eukaryotic expression vector pJW4303, whose 5' end is connected with tPA signal peptide sequence. The nucleic acid vaccine effectively stimulates the immune system after immunizing the mammal, and produces a better humoral immune response.

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being derived from a gene associated with activity of part of metabolism, said inducible enhancer.or activator sequence being derived from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regulator gene xlnR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and / or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

The invention discloses a method for improving yield of bacillus subtilis acetylglucosamine. According to the invention, knockout verification is carried out to obtain a result that key phosphatase YwpJ can catalyze GlcNAc-6-P to take a dephosphorylation effect to generate GlcNAc; by overexpressing the phosphatase YwpJ, excessive accumulation of intracellular sugar phosphates is reduced, a toxic effect of the excessively accumulated sugar phosphates on cells is eliminated, and the sugar phosphates are effectively secreted out of the cells, so that cell growth is improved; and GlcNAc syntheticprecursor degradation pathway key genes murQ and nagBB are further knocked out, degradation of a precusor substance is blocked, waste of carbon flow is reduced, more carbon flow is enabled to be transformed into products, a GlcNAc yield of a final recombinant strain FMIP34 reaches 26.1g / L and is increased by 61.1%, and a transformation rate of the GlcNAc reaches 0.483g per gram of glucose.

The present invention provides a fungal secretory expression vector, a construction method and application thereof. The fungal secretory expression vector is a fungal secretory expression vector 74HSP in which the extracellular signal peptide of the N-terminal 23aa (MAPYSMVLLGALSILGFGAYAQE) of the Six1d secretory protein is inserted into the 4665bp of the pCT74 vector. The present invention successfully constructs a fungal secretory expression vector that can effectively secrete plant proteins into the extracellular space of pathogenic bacteria, and realizes that the target protein is effectively expressed in fungi and secreted into plants during the infection process, thereby realizing the function of plant genes. Quick identification.

An eucaryotic expression of E.coli heat-labile enterotoxin B subunit, LT-B is prepared by defining nucleic acid endoenzyme site, connecting LT-B nucleic acid sequence with pPICZ alpha A to obtain recombinant expression carrier, inducing recombinant expression carrier into Pichiapastoris X-33 or KM71, and fermentation expressing to obtain target protein LT-B. It has high economical value.

The invention belongs to the technical field of moon cake production and particularly relates to a production method of a lemon cake type moon cake. The production method specially comprises the stepsas follows: (1) production of a moon cake wrapper; (2) production of moon cake filling; and (3) production of the lemon cake type moon cake: the moon cake wrapper is filled with the moon cake filling, the moon cake is placed in a mold, the mold is placed in a baking oven for baking and then taken out, demolding is conducted, and the lemon cake type moon cake is obtained. The obtained moon cake tastes fragrant, soft, unique and sweet and sour palatably and can promote balanced absorption of nutritional ingredients by the brain of an eater, promote melatonin secretion, effectively regulate natural sleep of the human body, overcome sleep disorders, improve sleep quality, resist oxidation, nourish brain cells, effectively help digestion, remove swelling, eliminate dampness, promote circulation of qi, warm stomach, promote gastric secretion, enhance metabolism capacity, effectively prevent and relieve constipation, clear heat, reduce internal heat, remove toxins and eliminate bad breath, the expiration date of the moon cake can be effectively prolonged, and the eating rate and the eating nutrition and healthcare effects can be improved.

The invention belongs to the pharmaceutical technical field and specifically relates to hydrosulphonyl benzoglioxaline derivatives which are shown in general formula (I) and contain dioxo heptane pyridine, isomers and pharmaceutically acceptable salts thereof, wherein, R<1>, R<2> and R<3> are defined as in the specification. The invention also relates to preparation methods of the compounds, drugcompositions containing the compounds and the application of the compounds in preparing drugs for preventing and / or treating digestive ulcer.

The invention relates to the technical field of veterinary biological products, in particular to antibacterial microcapsules for piglets. The antibacterial microcapsules are composed of, by weight, 15-20 parts of bacillus amyloliquefaciens, 5-10 parts of traditional Chinese medicine enhancer, 2-3 parts of kaolin, 5-7 parts of magnesium aluminum hydroxide, 8-12 parts of ethyl cellulose, 6-9 parts of methyl cellulose, 2-4 parts of butyl hydroxy anisole and 90-100 parts of ultrapure water. The antibacterial microcapsules can stimulate the piglets to generate immunity, help the piglets to establish an intestinal microecological system as soon as possible, prevent digestive tract structures of the piglets from being damaged, prevent fungi, viruses and pathogenic microorganisms from invading the piglets and prevent stress reaction of the piglets and have effects of improving stress resistance and disease resistance of the piglets and regulating body metabolism, morbidity and mortality can be reduced effectively, diarrhea of the piglets can be remarkably reduced, and growing performance of the piglets is improved.

The invention relates to the technical field of processing of feed additives, in particular to a feed additive capable of improving functions of the mammary gland of a camel and a preparation method of the feed additive. The feed additive disclosed by the invention consists of fatty acids, mango core essential oil, a glossy privet fruit extract, a black bean extract, a kelp extract and soy flour,wherein the fatty acids are fatty acids extracted autonomously by an applicant, and fatty acid components are reasonable in composition and can dredge the mammary gland; the mango core essential oil and the glossy privet fruit extract can achieve the effect of resisting oxidation; and the black bean extract and the soy flour can protect the ovarian function of animals and urge estrogen secretion.The additive disclosed by the invention realizes the purpose of protecting the functions of the mammary gland of the camel through mutual effects in two respects of dredging the mammary gland pipelines and urging hormone balance.

The invention belongs to the pharmaceutical technical field and specifically relates to imidazopyridine derivatives which are shown in general formula (I) and contain dioxide heterocyclic pyridine, pharmaceutically acceptable salts and isomers thereof, wherein, R<1>, R<2>, R<3> and n are defined as in the specification. The invention also relates to preparation methods of the compounds, drug compositions containing the compounds and the application of the compounds in preparing drugs for preventing and / or treating digestive ulcer.

The invention relates to the technical field of waistcoats, in particular to a magnetic waistcoat. The magnetic waistcoat comprises a waistcoat main body. A magnetic care cushion block capable of abutting against the body parts is arranged on the inner side of the waistcoat main body and comprises a basis cushion block body. Massage reinforcing ribs arranged at intervals are arranged on one side, close to the body parts, of the basis cushion block body, and the massage reinforcing ribs are in strip shapes and extend in the direction from the head to the feet; strip-shaped grooves with groove openings facing the body parts and extending in the direction from the head to the feed are located at the portions, between the adjacent massage reinforcing ribs, of the basis cushion block body, and at least a line of magnetic particles arranged at intervals in the direction from the head to the feet are arranged in the strip-shaped grooves. The magnetic waistcoat is stable in structure, comfortable in wearing and good in magnetic care effect.