44results about How to "Effective secretion" patented technology

A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant.Thus, it becomes possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.

The invention discloses a method for preparing soluble yeast glucan. After mixing powdered yeast glucan and grinding balls according to the mass ratio of 1:5~20, the planetary ball mill is used for processing under the condition of power 900~1200W After 5-30 minutes, the obtained yeast glucan was mixed with water at a mass ratio of 1:10-25 to dissolve the yeast glucan, and the insoluble matter was removed by centrifugation to obtain a yeast glucan solution. The method of the invention has the characteristics of simplicity, rapidity, high efficiency and cleanness, and the prepared yeast glucan has better water solubility and excellent immune activity.

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being derived from a gene associated with activity of part of metabolism, said inducible enhancer.or activator sequence being derived from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regulator gene xlnR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and / or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

The invention relates to the technical field of veterinary biological products, in particular to antibacterial microcapsules for piglets. The antibacterial microcapsules are composed of, by weight, 15-20 parts of bacillus amyloliquefaciens, 5-10 parts of traditional Chinese medicine enhancer, 2-3 parts of kaolin, 5-7 parts of magnesium aluminum hydroxide, 8-12 parts of ethyl cellulose, 6-9 parts of methyl cellulose, 2-4 parts of butyl hydroxy anisole and 90-100 parts of ultrapure water. The antibacterial microcapsules can stimulate the piglets to generate immunity, help the piglets to establish an intestinal microecological system as soon as possible, prevent digestive tract structures of the piglets from being damaged, prevent fungi, viruses and pathogenic microorganisms from invading the piglets and prevent stress reaction of the piglets and have effects of improving stress resistance and disease resistance of the piglets and regulating body metabolism, morbidity and mortality can be reduced effectively, diarrhea of the piglets can be remarkably reduced, and growing performance of the piglets is improved.