Application of soluble protein BAFF in B cell in-vitro culture and proliferation

A technology of B cells and cells, applied in the direction of cell culture active agents, NGF-receptor/TNF-receptor superfamily, animal cells, etc., can solve the problems that BAFF has not been introduced into human B cells in vitro expansion and long-term culture , to achieve the effect of promoting the expansion of human B cells in vitro and the effect of antigen presentation

Inactive Publication Date: 2016-12-14
SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to this special property of BAFF protein, BAFF has not been introduced into the system of in vitro expansion and long-term culture of human B cells

Method used

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  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation
  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation
  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation

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Embodiment Construction

[0024] 分泌型BAFF肽,其氨基酸序列由BAFF的133-285位和偶联在其N端的IL-2肽组成,BAFF的133-285位的氨基酸序列为:AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL,IL-2肽的氨基酸序列为: MYRMQLLSCIALSLAVTNS.

[0025] 作为示例,BAFF的133-285位对应的核苷酸序列为:gccgttcagggtccagaagaaacagtcactcaagactgcttgcaactgattgcagacagtgaaacaccaactatacaaaaaggatcttacacatttgttccatggcttctcagctttaaaaggggaagtgccctagaagaaaaagagaataaaatattggtcaaagaaactggttacttttttatatatggtcaggttttatatactgataagacctacgccatgggacatctaattcagaggaagaaggtccatgtctttggggatgaattgagtctggtgactttgtttcgatgtattcaaaatatgcctgaaacactacccaataattcctgctattcagctggcattgcaaaactggaagaaggagatgaactccaacttgcaataccaagagaaaatgcacaaatatcactggatggagatgtcacattttttggtgcattgaaactgctgtga(SEQ ID NO:3)。 The nucleotide sequence corresponding to the IL-2 peptide is: atgtacaggatgcaactcctgtcttgcattgcactaagtcttgcacttgtcacaaacagt (SEQ ID NO: 4).

[0026] An expression vector of a secreted BAFF peptide, which is inserted with...

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Abstract

The invention discloses application of soluble protein BAFF in B cell in-vitro culture and proliferation. Peptide expressed by secretory BAFF peptide can be effectively secreted out of cells to generate corresponding bioactivity. A cell line can continuously secrete water-soluble BAFF protein having bioactivity in the process of culture, the water-soluble BAFF protein serving as a tool cell and B cells are co-cultured, and in-vitro proliferation and antigen presentation effects of human B cells can be effectively improved.

Description

technical field [0001] The present invention relates to a cell construction method and application thereof, in particular to a construction method of secretory BAFF cell line and its application in improving the in vitro expansion and antigen presentation of human peripheral blood B cells. Background technique [0002] In recent years, because T cells play an important role in the immune system, more and more research groups are trying to use T cell adoptive therapy to treat tumors or viral infections. At present, many research groups at home and abroad are studying how to maintain the long-term culture of T cells in vitro, improve the survival time of T cells, and improve the immunological function of T cells for adoptive immunotherapy. When performing T cell therapy, the main strategy is to obtain a sufficient amount of T cells with killing function through clonal expansion first, which can be co-cultured with antigen-presenting cells. Compared with the construction of ar...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/85C12N5/0781
CPCC07K14/55C07K14/70575C12N5/0635C07K2319/03C12N2501/33C12N2501/231C12N2501/2304C12N2501/2302C12N2500/24A61K39/46449A61K2239/57A61K39/4622A61K39/4611A61K39/4612C07K19/00C12N2501/52C12N2501/25C07K14/525C07K14/70596C07K14/70578C07K2319/00C07K2319/43C07K2319/60C12N15/85
Inventor 张辉张译文高士麟
Owner SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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