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Application of soluble protein BAFF in B cell in-vitro culture and proliferation

A technology of B cells and cells, applied in the direction of cell culture active agents, NGF-receptor/TNF-receptor superfamily, animal cells, etc., can solve the problems that BAFF has not been introduced into human B cells in vitro expansion and long-term culture , to achieve the effect of promoting the expansion of human B cells in vitro and the effect of antigen presentation

Inactive Publication Date: 2016-12-14
SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to this special property of BAFF protein, BAFF has not been introduced into the system of in vitro expansion and long-term culture of human B cells

Method used

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  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation
  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation
  • Application of soluble protein BAFF in B cell in-vitro culture and proliferation

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Embodiment Construction

[0024] 分泌型BAFF肽,其氨基酸序列由BAFF的133-285位和偶联在其N端的IL-2肽组成,BAFF的133-285位的氨基酸序列为:AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL,IL-2肽的氨基酸序列为: MYRMQLLSCIALSLAVTNS.

[0025] 作为示例,BAFF的133-285位对应的核苷酸序列为:gccgttcagggtccagaagaaacagtcactcaagactgcttgcaactgattgcagacagtgaaacaccaactatacaaaaaggatcttacacatttgttccatggcttctcagctttaaaaggggaagtgccctagaagaaaaagagaataaaatattggtcaaagaaactggttacttttttatatatggtcaggttttatatactgataagacctacgccatgggacatctaattcagaggaagaaggtccatgtctttggggatgaattgagtctggtgactttgtttcgatgtattcaaaatatgcctgaaacactacccaataattcctgctattcagctggcattgcaaaactggaagaaggagatgaactccaacttgcaataccaagagaaaatgcacaaatatcactggatggagatgtcacattttttggtgcattgaaactgctgtga(SEQ ID NO:3)。 The nucleotide sequence corresponding to the IL-2 peptide is: atgtacaggatgcaactcctgtcttgcattgcactaagtcttgcacttgtcacaaacagt (SEQ ID NO: 4).

[0026] An expression vector of a secreted BAFF peptide, which is inserted with...

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Abstract

The invention discloses application of soluble protein BAFF in B cell in-vitro culture and proliferation. Peptide expressed by secretory BAFF peptide can be effectively secreted out of cells to generate corresponding bioactivity. A cell line can continuously secrete water-soluble BAFF protein having bioactivity in the process of culture, the water-soluble BAFF protein serving as a tool cell and B cells are co-cultured, and in-vitro proliferation and antigen presentation effects of human B cells can be effectively improved.

Description

technical field [0001] The present invention relates to a cell construction method and application thereof, in particular to a construction method of secretory BAFF cell line and its application in improving the in vitro expansion and antigen presentation of human peripheral blood B cells. Background technique [0002] In recent years, because T cells play an important role in the immune system, more and more research groups are trying to use T cell adoptive therapy to treat tumors or viral infections. At present, many research groups at home and abroad are studying how to maintain the long-term culture of T cells in vitro, improve the survival time of T cells, and improve the immunological function of T cells for adoptive immunotherapy. When performing T cell therapy, the main strategy is to obtain a sufficient amount of T cells with killing function through clonal expansion first, which can be co-cultured with antigen-presenting cells. Compared with the construction of ar...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/85C12N5/0781
CPCC07K14/55C07K14/70575C12N5/0635C07K2319/03C12N2501/33C12N2501/231C12N2501/2304C12N2501/2302C12N2500/24A61K39/46449A61K2239/57A61K39/4622A61K39/4611A61K39/4612C07K19/00C12N2501/52C12N2501/25C07K14/525C07K14/70596C07K14/70578C07K2319/00C07K2319/43C07K2319/60C12N15/85
Inventor 张辉张译文高士麟
Owner SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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