PCR primer ad kit for simultaneously detecting cyprinid herpesvirus I, cyprinid herpesvirus II and cyprinid herpesvirus III and application of PCR primer

Abstract

The invention discloses a PCR primer and a kit for simultaneously detecting cyprinid herpesvirus I, cyprinid herpesvirus II and cyprinid herpesvirus III and an application of the PCR primer. The primers include CyHV-F and CyHV-R, with sequences shown as SEQ ID NO: 1 and SEQ ID NO: 2. The primer provided by the invention, when used for detection, is free from a cross reaction with other viruses and the primer is low in false positive rate; and the three cyprinid herpesviruses, namely CyHV-I, CyHV-II and CyHV-III, can be rapidly, accurately and conveniently detected and distinguished simultaneously. The invention provides a molecular biological technical basis for the early diagnosis and the prevention and treatment of the cyprinid herpesviruses. The primer has broad applicability and practicability.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

AI Technical Summary

Benefits of technology

This patented technology describes methods and tools used during researches into identifying cycling agents called cyclistella (CY) sinensis). These techniques involve amplification or testing DNA from these organisms' genome. They provide fast, easy access, precise results at lower costs than traditional laboratory tests while also being able to identify various bacterial species like Escherichia coli O157: H7. Additionally, they allow scientists to study how certain chemical substances affect their ability to fight off disease caused by harmful microorganism such as Bacillaria spp., Plasmodium falciparum malaria parasites, etc.. Overall, this technique allows us to make faster, easier ways to diagnose diseases related to cytomegalovirus type 1(cyclidylinenomenon), including Chikungunya fever, Yellow Fever, Dengue hemorrhagie syndrome, Crimea, Lyme disease, West Nile encephalitis, SARS coronaviruse, influenza pandemics, tuberculosis, dengue fevers, chickenpox, polio epidemies, hepatitis A, rheumatoid arthritis, lupencepsemia, leprotopenia, sarcoidosis, cancer, necrotropic enterobiliary pancreatitis, lipid storage disorders, Parkinson’sdisease, amyotrophic lateral sclerostomy defective patients, cardiac failure, stroke, brain damage, spinal cord injury, muscle degeneration, multiple myeloma, Alzheimer′s disease, autosensory nerve injuries, pulmonary embolysms, respirator attacks, gastroenteropathisis, diarrhoea, urinary tract symptoms, urogenital abnormalities, central nervous systems problems associated therewith, especially those involving neuronal cells involved in cognitive function.

Problems solved by technology

This patents describes various technical problem addressed in this patented text relating to the spread of Cytospora sialonchisis due to its ability to cause severe damage to cropped crayfishes during their growth cycle. Current diagnoses involve visual inspections with blood smears from affected individuals followed by microscopic analysis. However, these tests require expensive equipment and trained operators who may miss important parts like young broods. Therefore, an improved assays system capable of quickly identifying different strains of bacterium called CyHv-III would provide better tools for understanding how Cytosiherapy treatments targeted against Fish Hydronephritis affect wild populations around the globe.

Images