A baculovirus vector-based carp herpes virus type II dna vaccine and its construction method and application

A baculovirus vector, carp herpes virus technology, applied in the field of herpes virus type II DNA vaccine, can solve the problems of poor immune protection effect, fish body injury and stress response, poor immune effect, etc.
CN108728490BActive Publication Date: 2021-03-26苏州培恩特生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
苏州培恩特生物科技有限公司
Publication Date
2021-03-26

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Abstract

The invention relates to a cyprinid herpesvirus II DNA vaccine based on a baculovirus vector as well as a building method and application thereof. Codons of regional sequences 1-186, 993-1197, 603-783and 85-186 nt of cyprinid herpesvirus II ORF72, ORF66, ORF81 and ORF82 are optimized; then, ORF72-ORF66-ORF81-ORF82 fusion sequences are chemically synthesized; next, the promoter control is performed; the clone into the baculovirus transfer vector pFSATBacTMDual is performed to build recombinant plasmids pFSATBacTMDual-FA-D4ORF; after the plasmids are used for converting DH10 / Bac competent cells, Bacmid-FA-D4ORF is obtained; after the Bacmid-FA-D4ORF DNA is used for transfecting silkworm culture cells, BmNPV-FA-D4ORF is obtained and is the cyprinid herpesvirus II DNA vaccine based on the baculovirus vector. The DNA vaccine can realize the immune on carassius auratus gibelio by using an injection or oral administration or soaking method; the gill bleeding diseases of the carassius auratusgibelio can be reduced.
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Description

technical field

[0001] The invention relates to the technical field of viral genetic engineering, in particular to a method and application for preparing a carp herpesvirus type II DNA vaccine based on a baculovirus vector. Background technique

[0002] Cyprinid herpesvirus II (CyHV-2) infection can cause gill hemorrhage outbreaks in heterogeneous gibel crucian carp, resulting in serious economic losses. At present, the prevention and control of the disease is mainly carried out from the aspects of seed detection, enhancement of immunity, reduction of stress, reduction of breeding density, removal of diseased fish, etc., but the prevention and control effect is limited. Immunoprophylaxis is the most direct and effective method for the prevention and treatment of viral diseases. At present, common vaccines mainly include: inactivated vaccines (killed vaccines), attenuated vaccines, subunit vaccines, DNA vaccines, and protein subunit / DNA combined vaccines. Different types of...

Claims

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