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Factory seedling cultivating method of actinidia arguta

A soft jujube kiwifruit, industrialized technology, applied in the field of plant tissue culture, can solve the problems of the survival rate of seedlings that are easy to carry bacteria, difficult to maintain the characteristics of the mother plant, slow seedling propagation, etc. , the effect of convenient sampling

Inactive Publication Date: 2011-09-14
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is mainly aimed at the wild state of kiwi fruit in Northeast China. Through traditional propagation methods including sowing propagation, cutting propagation, and grafting propagation, there will be serious damage to resources, slow seedling propagation, difficult to maintain the properties of the mother plant, and easy to carry bacteria. In order to solve the problem of low survival rate of seedlings, a method for industrialized seedling raising of Actinidia jujube is proposed, the purpose of which is to shorten the seedling cycle, reduce the cost of seedling raising, improve the survival rate of seedlings, reduce plant infection, maintain the excellent traits of the mother plant, and solve the problem of variety degradation problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of culture medium:

[0029] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 7.0g / L, white sugar is used instead of sucrose, the concentration is 28g / L, and the pH value of the basic medium is adjusted is 5.8;

[0030] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.0mg / L;

[0031] 3) Subculture medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 2.5mg / L and IBA (indolebutyric acid) to make the concentration 1.25mg / L;

[0032] (2) Culture conditions: the temperature in the culture room is controlled at 25°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;

[0033] (3) Selection and disinfection of explants:

[0034] 1) Selection of explants: Select the dormant branches of Actin...

Embodiment 2

[0040] (1) Preparation of culture medium:

[0041] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 6.0g / L, white sugar is used instead of sucrose, the concentration is 30g / L, and the pH value of the basic medium is adjusted is 5.5;

[0042]2) Induction medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 0.5mg / L;

[0043] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.5mg / L and IBA (indolebutyric acid) to make the concentration 1.5mg / L;

[0044] (2) Culture conditions: the temperature in the culture room is controlled at 24°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;

[0045] (3) Selection and disinfection of explants:

[0046] 1) Selection of explants: Select the dormant branches of Actinid...

Embodiment 3

[0052] (1) Preparation of culture medium:

[0053] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 8.0g / L, white sugar is used instead of sucrose, the concentration is 25g / L, and the pH value of the basic medium is adjusted is 6.0;

[0054] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.5mg / L;

[0055] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 3.2mg / L and IBA (indolebutyric acid) to make the concentration 1.0mg / L;

[0056] (2) Culture conditions: the temperature in the culture room is controlled at 26°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;

[0057] (3) Selection and disinfection of explants:

[0058] 1) Selection of explants: Select the dormant branches of Actini...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a factory seedling cultivating method of actinidia arguta. The method is mainly technically characterized by comprising the five steps of: preparing culture media, selecting and disinfecting explants, inoculating, subculturing and transplanting in a sand bed. The factory seedling cultivating method of actinidia arguta is mainly provided on account of the conditions that actinidia arguta is in a wild state in all northeast areas, and the traditional cultivating modes which comprise sowing propagation, cuttage propagation and grafting propagation cause the problems that the variation of offspring characters is serious, male plants are difficult to separate from female plants, resources are seriously damaged, the seedling cultivating speed is low, the characters of the female plants are difficult to keep, the plants are easily infected with bacteria, the survival rate of the seedlings is low, and the like. Induced buds are subcultured and then directly transplanted in the sand bed, the rooting culture process is omitted, the seedling cultivating period is shortened by 50-60 days, the seedling cultivating cost is greatly lowered, the survival rate of the seedlings is obviously increased, the plant infection rate is reduced, excellent female plant characters are kept, and the variety degrading problem is solved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to an industrial seedling raising method of Actinidia jujube. Background technique [0002] Jujube kiwifruit has the reputation of the king of fruit Vc. The fruit contains about 15% soluble solids, 8.8% total sugar, 1.5% organic acid, Vc 430mg / 100g is 100 times that of apple, amino acid 933.9mg / 100g, jujube kiwifruit juice It has the function of blocking the synthesis of carcinogenic nitroso compounds, and also contains hydrolase of thiol protease and superoxide dismutase (SOD), which has the functions of beautifying, improving immune function, anti-cancer, anti-aging, softening blood vessels, anti-tumor and anti-inflammation Its function, nutrition and health value are extremely high. In recent years, kiwi fruit has received great attention from researchers and producers. However, because most of kiwi fruit is in the wild and grows in some mountainous areas and wild r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘长江孙晓荣梁爽谭昌华
Owner SHENYANG AGRI UNIV
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