Factory seedling cultivating method of actinidia arguta

A soft jujube kiwifruit, industrialized technology, applied in the field of plant tissue culture, can solve the problems of the survival rate of seedlings that are easy to carry bacteria, difficult to maintain the characteristics of the mother plant, slow seedling propagation, etc. , the effect of convenient sampling

Inactive Publication Date: 2011-09-14
SHENYANG AGRI UNIV
View PDF1 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is mainly aimed at the wild state of kiwi fruit in Northeast China. Through traditional propagation methods including sowing propagation, cutting propagation, and grafting propagation, there will be serious damage to resources, slow seedling propagation, difficult to maintain the properties of the mother plant, and easy to carry bacteria. In order to solv

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1:

[0028] (1) Preparation of culture medium:

[0029] 1) Basic medium: Use conventional MS medium formula to prepare, replace distilled water in the formula with ordinary tap water, in which the agar concentration is 7.0g / L, and white sugar is used to replace sucrose, the concentration is 28g / L, adjust the pH value of the basic medium Is 5.8;

[0030] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 1.0mg / L;

[0031] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 2.5mg / L and IBA (indolebutyric acid) to make the concentration 1.25mg / L;

[0032] (2) Cultivation conditions: The temperature in the culture room is controlled at 25°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected with ultraviolet light once a week;

[0033] (3) Selection and disinfection of explants:

[0034] 1) Selection of explants: select dormant branches of ...

Example Embodiment

[0039] Example 2

[0040] (1) Preparation of culture medium:

[0041] 1) Basic medium: Use conventional MS medium formula to prepare, replace distilled water in the formula with ordinary tap water, where the agar concentration is 6.0g / L, and replace sucrose with white sugar, the concentration is 30g / L, adjust the pH value of the basic medium Is 5.5;

[0042] 2) Induction medium: Add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 0.5mg / L;

[0043] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 1.5mg / L and IBA (indolebutyric acid) to make the concentration 1.5mg / L;

[0044] (2) Cultivation conditions: the temperature in the culture room is controlled at 24°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected with ultraviolet light once a week;

[0045] (3) Selection and disinfection of explants:

[0046] 1) Selection of explants: select dormant branches of Actinidia a...

Example Embodiment

[0051] Example 3

[0052] (1) Preparation of culture medium:

[0053] 1) Basic medium: Use conventional MS medium formula to prepare, replace distilled water in the formula with ordinary tap water, where the agar concentration is 8.0g / L, replace sucrose with white sugar, the concentration is 25g / L, adjust the pH value of the basic medium 6.0;

[0054] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 1.5mg / L;

[0055] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the minimal medium to make the concentration 3.2mg / L and IBA (indolebutyric acid) to make the concentration 1.0mg / L;

[0056] (2) Cultivation conditions: The temperature in the culture room is controlled at 26°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected with ultraviolet light once a week;

[0057] (3) Selection and disinfection of explants:

[0058] 1) Selection of explants: select dormant branches of Actinidia arguta, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a factory seedling cultivating method of actinidia arguta. The method is mainly technically characterized by comprising the five steps of: preparing culture media, selecting and disinfecting explants, inoculating, subculturing and transplanting in a sand bed. The factory seedling cultivating method of actinidia arguta is mainly provided on account of the conditions that actinidia arguta is in a wild state in all northeast areas, and the traditional cultivating modes which comprise sowing propagation, cuttage propagation and grafting propagation cause the problems that the variation of offspring characters is serious, male plants are difficult to separate from female plants, resources are seriously damaged, the seedling cultivating speed is low, the characters of the female plants are difficult to keep, the plants are easily infected with bacteria, the survival rate of the seedlings is low, and the like. Induced buds are subcultured and then directly transplanted in the sand bed, the rooting culture process is omitted, the seedling cultivating period is shortened by 50-60 days, the seedling cultivating cost is greatly lowered, the survival rate of the seedlings is obviously increased, the plant infection rate is reduced, excellent female plant characters are kept, and the variety degrading problem is solved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to an industrial seedling raising method of Actinidia jujube. Background technique [0002] Jujube kiwifruit has the reputation of the king of fruit Vc. The fruit contains about 15% soluble solids, 8.8% total sugar, 1.5% organic acid, Vc 430mg / 100g is 100 times that of apple, amino acid 933.9mg / 100g, jujube kiwifruit juice It has the function of blocking the synthesis of carcinogenic nitroso compounds, and also contains hydrolase of thiol protease and superoxide dismutase (SOD), which has the functions of beautifying, improving immune function, anti-cancer, anti-aging, softening blood vessels, anti-tumor and anti-inflammation Its function, nutrition and health value are extremely high. In recent years, kiwi fruit has received great attention from researchers and producers. However, because most of kiwi fruit is in the wild and grows in some mountainous areas and wild r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 刘长江孙晓荣梁爽谭昌华
Owner SHENYANG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap