Factory seedling cultivating method of actinidia arguta
A soft jujube kiwifruit, industrialized technology, applied in the field of plant tissue culture, can solve the problems of the survival rate of seedlings that are easy to carry bacteria, difficult to maintain the characteristics of the mother plant, slow seedling propagation, etc. , the effect of convenient sampling
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Embodiment 1
[0028] (1) Preparation of culture medium:
[0029] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 7.0g / L, white sugar is used instead of sucrose, the concentration is 28g / L, and the pH value of the basic medium is adjusted is 5.8;
[0030] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.0mg / L;
[0031] 3) Subculture medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 2.5mg / L and IBA (indolebutyric acid) to make the concentration 1.25mg / L;
[0032] (2) Culture conditions: the temperature in the culture room is controlled at 25°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;
[0033] (3) Selection and disinfection of explants:
[0034] 1) Selection of explants: Select the dormant branches of Actin...
Embodiment 2
[0040] (1) Preparation of culture medium:
[0041] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 6.0g / L, white sugar is used instead of sucrose, the concentration is 30g / L, and the pH value of the basic medium is adjusted is 5.5;
[0042]2) Induction medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 0.5mg / L;
[0043] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.5mg / L and IBA (indolebutyric acid) to make the concentration 1.5mg / L;
[0044] (2) Culture conditions: the temperature in the culture room is controlled at 24°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;
[0045] (3) Selection and disinfection of explants:
[0046] 1) Selection of explants: Select the dormant branches of Actinid...
Embodiment 3
[0052] (1) Preparation of culture medium:
[0053] 1) Basic medium: Prepared by conventional MS medium formula, using ordinary tap water instead of distilled water in the formula, in which the agar concentration is 8.0g / L, white sugar is used instead of sucrose, the concentration is 25g / L, and the pH value of the basic medium is adjusted is 6.0;
[0054] 2) Induction medium: add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 1.5mg / L;
[0055] 3) Subculture medium: Add 6-BA (6-benzylaminopurine) to the basic medium to make the concentration 3.2mg / L and IBA (indolebutyric acid) to make the concentration 1.0mg / L;
[0056] (2) Culture conditions: the temperature in the culture room is controlled at 26°C, the light cycle is 12 hours / d, the light intensity is 2000 lux, and the room is disinfected once a week with ultraviolet rays;
[0057] (3) Selection and disinfection of explants:
[0058] 1) Selection of explants: Select the dormant branches of Actini...
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