Culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting

A kind of technology of Acacia marina and out-of-the-vessel rooting, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low survival rate of rooted seedlings transplanted, long rooting time, low rooting rate, etc., to achieve Realize the effect of industrialized seedling cultivation, speed up the process and improve the quality

Inactive Publication Date: 2014-04-23
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the rooting technology in the bottle of Ma Da hybrid acacia has disadvantages such as low rooting rate, long rooting time, and low survival rate of rooted seedlings after transplanting.

Method used

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  • Culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting
  • Culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting
  • Culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting

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Embodiment 1

[0029] 1. Rooting outside the bottle

[0030] 1) Materials: AMA12001, a cross-breeding Acacia acacia with straight stem shape, large growth volume and wind resistance, was selected, and the axillary buds of the new shoots of the year were used as explants, sterilized, and the stems were inserted into the primary culture medium (for MS 30-40 g / L sucrose and 6-7 g / L agar are added to the basic medium) for primary culture for 30-35 days, so that the axillary buds grow to about 3 cm in height, and the culture conditions are: temperature 23-27 ℃, The photoperiod is 13-15 h light / 9-11 h dark, and the light intensity is 2000-2500 Lx;

[0031] The axillary buds after the primary culture were cut and inoculated into the proliferation medium (1.5 mg / L 6-BA, 0.1 mg / L NAA, 0.1 g / L activated carbon, 30 g / L sucrose and 7 g / L L agar), and the rooting outside the bottle was selected for multiplication and cultured for 45 days, so that the axillary buds grew into multiplication seedlings wi...

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Abstract

The invention discloses a culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting. The method disclosed by the invention comprises the following steps: scissoring an Acacia mangium*A. auriculiformis Cunn.ex Bench axillary bud containing stem, and grafting the stem into a primary culture medium; culturing the stem until the height of the axillary bud is 2-3 cm, scissoring the axillary bud, grafting the axillary bud into an enrichment culture medium, and culturing the axillary bud until an enrichment seedling with a height of 4-5 cm is obtained; hardening the enrichment seedling for 5-7 days under natural lighting, and scissoring the seedling into single buds; and after the roots of the buds are soaked for 50-70 min by using 100-150 mg/LIBA, putting the buds into a nutritious cup in a greenhouse to culture by cottage. According to the ex-vitro rooting method disclosed by the invention, a rooting process of an enrichment seedling and a domestication process are combined together, so that the quality and survival rate of tissue culture seedlings are improved, the process of seeding breeding is accelerated, the whole tissue culture link is simplified, the production time is shortened, the culturing space of tissue culture is saved, and the production cost is reduced, thereby facilitating the factory-like seedling raising of Acacia mangium*A. auriculiformis Cunn.ex Bench.

Description

technical field [0001] The invention relates to a rooting culture method for plant tissue culture seedlings, in particular to a rooting method for out-of-the-vessel rooting of the Ma Da hybrid acacia. Background technique [0002] Acacia ( Acacia ) is an important fast-growing tree species introduced from Australia in my country, belonging to Mimosaceae ( Mimosaceae ) Acacia ( Acacia ), can symbiotically fix nitrogen with rhizobia in the soil and can survive in nitrogen-poor, acidic, or even degraded soil conditions. role and status. Among them, Acacia Martha ( Acacia mangium × A. auriculiformis ) , is Mazhan Acacia ( Acacia mangium ) as the female parent, Daye Acacia ( Acacia. auriculiformis ) is naturally bred from the male parent. It not only has the advantages of straight trunk and small branches of Acacia danifolia, but also has the fast-growing performance of Acacia mazhan. It not only has the economic value of industrial papermaking and furniture decoration,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 胡峰黄烈健施琼
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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