Detoxification and propagation expanding method of sweet potatoes
A technology for sweet potatoes and potato seedlings, applied in horticultural methods, botanical equipment and methods, root crop cultivation, etc., can solve problems such as quality reduction, production reduction, and variety degradation, so as to improve quality and output, enhance epidemic resistance, prevent Effect of Variety Quality Deterioration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] A method for virus-free and multiplication of sweet potatoes, the method for virus-free and multiplication mainly includes explant collection and disinfection treatment, differentiation and induction culture, multiplication culture, rooting culture, hardening and transplanting, field management and timely harvesting , including the following steps:
[0028] (1) Explant collection and disinfection treatment: choose healthy potato seedlings without diseases and insect pests as explants, cut off the stems and tendrils with stem tips, remove the visible leaves, and wash them directly with tap water for 1 to 1.5 hours. and filter dry with sterile gauze; then put it on the aseptic operating table, first wipe it once with sterile absorbent cotton soaked in 75% alcohol, after wiping, fully soak it in 75% alcohol for 30-40 seconds, take it out and use the weight Sterilize with mercuric chloride solution with a percentage of 0.1% for 10 to 15 minutes, and finally rinse with steri...
Embodiment 2
[0037] A method for virus-free and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer, The compound fertilizer that urea and organic fertilizer are mixed forms, spreads 1300 kilograms of plant ash per mu, 40 kilograms of ternary compound fertilizers, 20 kilograms of urea and 1000 kilograms of organic fertilizers; The MS culture medium of mg / L kinetin KT, 0.08 mg / L NAA and 1.5mg / L BAP, described MS culture medium also contains the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates MS The pH of the medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.08mg / LNAA and 0.8mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adjust the pH of the MS ...
Embodiment 3
[0039] A method for detoxification and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, wherein in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer , urea and the compound fertilizer that organic fertilizer mixes to form, spread 1500 kilograms of plant ash by every mu, 45 kilograms of ternary compound fertilizers, 25 kilograms of urea and 1200 kilograms of organic fertilizers; The MS culture medium of 0.1 mg / L kinetin KT, 0.1 mg / L NAA and 2.0 mg / L BAP, described MS culture medium also comprises the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates The pH of the MS medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.1 mg / LNAA and 1.0 mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adj...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com