Fast reproduction method for energy willow tissue culture

A tissue culture, rapid technique used in the field of plant tissue culture

Inactive Publication Date: 2014-04-23
镇江山水湾生态农业开发有限公司
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Problems solved by technology

At present, the large-scale promotion of energy willow afforestation still lacks sufficient germplasm resources that are suitable for different regional climate conditions. Therefore, the introduction of Swedish energy willow is carried out to es

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Embodiment Construction

[0016] The tissue culture rapid propagation method of energy willow of the present invention will be further described in detail below in combination with specific embodiments.

[0017] The energy willow tissue culture rapid propagation method of the present invention comprises the following steps:

[0018] A: Selection of explants: The energy willow variety is Suliu 799 imported from Sweden, and the stem section with buds in the current year is selected;

[0019] B. Disinfection of explants: remove the leaves of the stems of the explants, and rinse them under running water for 3 hours, then place the rinsed branches in an ultra-clean workbench, clean them with 75% ethanol solution for 30 seconds, and then rinse with 75% ethanol solution. Rinse once with sterile water, then disinfect with 0.1% mercuric chloride for 7 minutes, rinse with sterile water 4-5 times, and finally dry the water with filter paper for later use;

[0020] C. Primary culture: Use a scalpel to cut the exp...

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Abstract

The invention discloses a fast reproduction method for energy willow tissue culture, which comprises the following steps: (A) selection of an explant; (B) disinfection of the explant: removing the leaves of the steam of the explant, placing the washed branch into a clean bench, cleaning in an ethanol solution, washing with sterile water, disinfecting with mercury chloride and flushing with sterile water; (C) primary culture; (D) enrichment culture: introducing the primary-culture stem in good growth into an enrichment culture medium; (E) rooting culture; and (F) seedling hardening and transplanting: opening a bottle cover of a rooting culture medium, culturing at room temperature, taking out the seedling, and transplanting to a vermiculite turfy soil matrix. In the method, by adopting reasonable and scientific culture method and steps and controlling reasonable parameters, the quality of the cultured energy willow tissue is very good, the sound field of the energy willow is fast, and an important guarantee is provided for realizing large-scale industrialized seedling production of energy willow and accelerating the popularization and application of good germ plasm.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a rapid propagation method of energy willow tissue culture. Background technique [0002] The energy willow introduced from Sweden is a promising energy plant with strong adaptability, wide cultivation area and great commercial potential. All countries in the world pay great attention to the introduction of energy forest plants. The new varieties of energy willow cultivated in Sweden have become the propagation materials of energy forests that many countries are vying to introduce. Breakthroughs have also been made in the utilization technology, with great potential for development. [0003] In China, the introduction experiment of Swedish willow has been carried out in a few areas and has been successfully planted, and further experimental research is needed for widespread application. At present, the large-scale promotion of energy willow afforestation still lacks sufficient ge...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 裴乐乐张留恩吴珊珊金波昌张志永
Owner 镇江山水湾生态农业开发有限公司
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