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Tissue culture rapid propagation method of Emei anoectochilus formosanus

A technique for rapid propagation of A. emei Aurea, tissue culture, applied in the field of plant tissue culture, can solve the problems of slow natural reproduction and growth, harsh growth conditions, no germination ability, etc., achieve excellent medicinal properties, low production cost, and simple and easy method line effect

Inactive Publication Date: 2014-04-02
四川省自然资源科学研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, because the seeds of the clematis plant are tiny, do not have endosperm, and have no germination ability, they can only germinate under the symbiotic situation with fungi. Therefore, the germination rate is low, the wild growth conditions are harsh, and natural reproduction and growth are slow.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Materials Emei clematis, collected from Mount Emei, Sichuan. Propagate Emei golden clematis according to the following steps.

[0038] 1. Take the wild Amethyst emei plant, and cut off a 0.5cm long section containing an axillary bud.

[0039] 2. Rinse with tap water for 2-3 hours, clean with a soft brush, rinse with distilled water, place in an ultra-clean workbench, soak in 75% alcohol for 30 seconds, clean with sterile water, and disinfect the surface in 0.1% mercury solution for 15 minutes , wash with sterile water 4-5 times, 2 minutes each time.

[0040] 3. The sterilized explants were inoculated into the induction medium for cultivation; the induction medium was: MS medium + sucrose 30g / L + agar 8g / L + activated carbon 2.0g / L + 1.5mg / L6-BA + 0.5mg / LNAA , the pH value is 5.8; the culture period is 30-40 days; the culture conditions are: the light intensity is 2000lux, 16h / d, and the culture temperature is 22-25°C. After 7 days of cultivation, germination begins. ...

Embodiment 2

[0045] Materials Emei clematis, collected from Mount Emei, Sichuan. Propagate Emei golden clematis according to the following steps.

[0046] 1. Take the wild Amethyst emei plant and cut off a 0.5cm long section containing a terminal bud.

[0047] 2. Rinse with tap water for 2-3 hours, clean with a soft brush, rinse with distilled water, place in an ultra-clean workbench, soak in 75% alcohol for 30 seconds, clean with sterile water, and disinfect the surface in 0.1% mercury solution for 15 minutes , wash with sterile water 4-5 times, 2 minutes each time.

[0048] 3. The sterilized explants were inoculated into the induction medium for cultivation; the induction medium was: MS medium + sucrose 30g / L + agar 8g / L + activated carbon 2.0g / L + 1.5mg / L6-BA + 0.5mg / LNAA , the pH value is 5.8; the culture period is 30-40 days; the culture conditions are: the light intensity is 2000lux, 16h / d, and the culture temperature is 22-25°C. After about 7 days of cultivation, germination begi...

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Abstract

The invention discloses a tissue culture rapid propagation method of anoectochilus formosanus, and belongs to the technical field of plant tissue culture. By adopting the tissue culture rapid propagation method, the survival rate of transplanting and the growth coefficient can be increased, and the good characters and the good drug properties of the Emei anoectochilus formosanus can be kept. The tissue culture rapid propagation method comprises the following steps: selecting wild Emei anoectochilus formosanus growing in May to October, preparing an explant, carrying out disinfection treatment, inducing cluster buds, carrying out secondary culture and rooting and strengthening culture, and transplanting to greenhouses for culturing, and rapidly propagating the anoectochilus formosanus through hormone induction. The tissue culture rapid propagation method can be used for ensuring the good characters and the good drug properties of the Emei anoectochilus formosanus, is simple and low in cost and can realize industrialized seedling production. The percentage of inducing the cluster buds and rooting by adopting the method reaches above 90%, the survival rate of transplanting is above 95%, and the growth coefficients within four months can reach 8-10 times. The Emei anoectochilus formosanus produced by adopting the method does not mutate, is high in yield, low in cost and short in period and has relatively strong market competitiveness.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method for tissue culture and rapid propagation of A. emeiensis, which can improve the survival rate and multiplication coefficient of transplanting, and can maintain the excellent traits and medicinal properties of A. emei. Background technique [0002] Anoectochilus emeiensis K.Y.Lang. is a perennial herbaceous plant of Orchidaceae and genus Anoectochilus. Clematis. It is often used as medicine in the folk, and its amino acid composition, ingredients, content, vitamins, polysaccharides, etc. are higher than those of American ginseng and wild ginseng. It is flat in nature and sweet in taste, has the functions of clearing heat, cooling blood, dispelling wind and dampness, strengthening the heart and diuresis, strengthening the kidney and calming the liver, and lowering blood pressure. It has good effects in treating liver diseases, hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 鲁松熊铁一
Owner 四川省自然资源科学研究院
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