Culture medium for coriaceous euonymus tissue culture and method thereof

A tissue culture and culture medium technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of the survival rate of primary culture and the low survival rate of transplanting, and achieve the improvement of the survival rate and the survival rate of transplanting. good technical effect

Active Publication Date: 2017-01-18
河南红枫生物高科股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But adopt above-mentioned culture medium to carry out the tissue culture of Euonymus leatherus, the survival rate of its primary culture, the survival rate of transplanting are all low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Preparation of medium formula

[0062] (1) Mother liquor preparation

[0063] Mother liquor one: NH 4 NO 3 16.5g / L, KNO 3 19g / L, KH 2 PO 4 17g / L, MgSO 4 ·7H 2 O 3.7g / L;

[0064] Mother liquor two: CaCl 2 0.6644g / L;

[0065] Mother liquor three: KI 0.083g / L, MnSO 4 ·H 2 O 1.69g / L, ZnSO 4 ·7H 2 O 0.86g / L, H 3 BO 3 0.62g / L, Na 2 MoO 4 2H 2 O 0.025g / L, CuSO 4 ·5H 2 O 2.5g / L CoCl 2 ·6H 2 O 2.5g / L;

[0066] Mother liquor four: FeSO 4 ·7H 2 O 2.78g / L, Na 2 EDTA 3.73g / L;

[0067] Mother liquor five: inositol 2g / L, IVB niacin 0.01g / L, VB 6 0.01g / L, VB 1 0.002g / L, glycine 0.04g / L;

[0068] Reagent 1: 6BA 1g / L;

[0069] Reagent 2: NAA 1g / L;

[0070] Reagent 3: KT 1g / L;

[0071] Reagent 4: TDZ 0.1g / L;

[0072] Reagent 5: IBA 1g / L;

[0073] Reagent 6: HCL 0.5mol / L;

[0074] Reagent 7: NaOH 0.5mol / L;

[0075] Reagent 8: NaClO 0.1%;

[0076] Reagent 9: 0.1% mercury liter.

[0077] Mother liquor 1, mother liquor 2, mother liquor 3, mother...

Embodiment 2

[0121] 1. Explant treatment

[0122] Choose the stem section of Euonymus leatherensis with one or two lateral buds in the current year as explants. Soak and wash in clean water with detergent to remove residual insect eggs and stains on the explants, transfer them to a beaker, cover the mouth of the cup with clean gauze, and rinse under running water for 50-60 minutes.

[0123] Soak the explants in pre-prepared reagent 8 for 15 minutes, and gently scrub the joints between the axillary buds and the stems with a toothbrush.

[0124] Afterwards, remove the leaves from the stems and cut them into 1-2cm lengths in a plate, and set aside.

[0125] Before inoculation, turn on the ultra-clean bench UV lamp for 15-20 minutes, and then ventilate for 10 minutes. Then the explants were processed on the ultra-clean workbench according to the following steps: alcohol 30s → sterile water 2 times for 2 minutes → 0.1% mercury liter for 8 minutes → sterile water 2 times for 2 minutes → change...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a culture medium for coriaceous euonymus tissue culture and a method thereof. The culture medium for coriaceous euonymus tissue culture comprises an initial culture medium, a proliferation culture medium and a rooting culture medium. The culture medium can remarkably improve the survival rate and transplanting survival rate of the initially cultured coriaceous euonymus, the initiated culture survival rate is improved from existing below 30% to 85%, and the transplanting survival rate of seedlings is improved from existing 50% to existing above 90%. By utilization of the plant tissue culture method, the problems of long period and low reproduction rate of a traditional coriaceous euonymus reproduction mode are solved, a rapid coriaceous euonymus reproduction and culture system is formed, and industrialized seedling raising can be achieved.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a culture medium and a method for tissue culture of Euonymus lanceolata. Background technique [0002] Euonymus lecleri Lévl. shrub or small tree, 1-7 meters high. Euonymus leather leaf belongs to Euonymus genus Euonymus, which is a unique plant in China. Distributed in Hubei, Hunan, Sichuan, Guizhou and other places, it grows in areas with an altitude of 1,300 meters to 2,900 meters, and mostly grows in mountain forests and ditch sides. Plants of the genus Euonymus are widely used in garden applications, and are mostly used for solitary planting, cluster planting and group planting. They are excellent garden ornamental plants and greening tree species. As an evergreen tree species, Euonymus leather is resistant to shade and cold, and has strong stress resistance. It has high ornamental and application value in northern regions. With the gradual maturity of "south trees...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂郭冠男孟伟芳邓坤
Owner 河南红枫生物高科股份有限公司
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