Method for cultivating anoectochilus formosanus by using culture bag

A technology for cultivating bags and clematis, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of large space occupied by glass culture bottles, easy breakage of glass culture bottles, limited number of culture bottles, etc., and achieve the goal of inducing The effect of shortening the time, increasing the induction rate, improving the rooting rate and root quality

Inactive Publication Date: 2016-06-08
NANPING HUAKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glass culture bottles are fragile and difficult to carry, and because glass culture bottles take up a lot of space, the number of culture bottles to be processed at one time is limited when it is sterilized, which will reduce its production efficiency
More importantly, since the culture bottle and bottle ...

Method used

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  • Method for cultivating anoectochilus formosanus by using culture bag
  • Method for cultivating anoectochilus formosanus by using culture bag
  • Method for cultivating anoectochilus formosanus by using culture bag

Examples

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Effect test

Embodiment 1

[0030] 1) A transparent sealed plastic bag made of polypropylene material is used as the culture bag. The height of the bag is 14cm, the bottom length is 5cm, and the bottom width is 4cm; On the side wall of the body, a breathable filter device is provided at 6cm away from the mouth of the bag; the breathable filter device includes a tubular body used to communicate with the inner cavity of the bag and the atmosphere, and its height is 4mm, and the diameter of the nozzle is 3.5mm; the tubular body The outer end is provided with a disk-shaped body for fixing it on the bag body, and its diameter is 12 mm; the disk-shaped body is provided with a bacteria-filtering breathable membrane with a diameter of 8 mm and a pore size of 0.18 μm at the communication port between the tubular body and the atmosphere; The culture bag is placed in the supporting frame, and then put into the outer packaging bag for high temperature sterilization (such as figure 1 , 2 , 3);

[0031] 2) In the me...

Embodiment 2

[0038] 1) A transparent sealed plastic bag made of polypropylene material is used as the culture bag. The height of the bag is 16cm, the bottom length is 7cm, and the bottom width is 5cm; On the side wall of the bag body, a breathable filter device is provided at a place 8cm away from the mouth of the bag; the breathable filter device includes a tubular body used to communicate with the inner cavity of the bag and the atmosphere, its height is 5mm, and the diameter of the nozzle is 3.8mm; the tubular body The outer end of the outer end is provided with a disc-shaped body for fixing it on the bag body, and its diameter is 13 mm; the disc-shaped body is provided with a bacteria-filtering gas-permeable membrane with a diameter of 9 mm and a pore size of 0.2 μm at the communication port between the tubular body and the atmosphere; Place the culture bag in the support frame, and then put it into the outer packaging bag for high temperature sterilization;

[0039] 2) In the medium o...

Embodiment 3

[0046] 1) A transparent sealed plastic bag made of polypropylene material is used as the culture bag. The height of the bag is 18cm, the bottom length is 8cm, and the bottom width is 7cm; On the side wall of the bag body, there is a breathable filter device 10cm away from the mouth of the bag; the breathable filter device includes a tubular body used to communicate with the inner cavity of the bag and the atmosphere, the height of which is 6mm, and the diameter of the nozzle is 4.0mm; the tubular body The outer end of the outer end is provided with a disc-shaped body for fixing it on the bag body, and its diameter is 14 mm; the disc-shaped body is provided with a bacteria-filtering gas-permeable membrane with a diameter of 10 mm and a pore size of 0.22 μm at the communication port between the tubular body and the atmosphere; Place the culture bag in the support frame, and then put it into the outer packaging bag for high temperature sterilization;

[0047] 2) In the medium of ...

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Abstract

The invention discloses a method for cultivating anoectochilus formosanus by using a culture bag. A transparent sealing plastic bag is used for replacing a traditional glass culture bottle to serve as a culture container; and according to different growth phases of the anoectochilus formosanus, an induction culture medium, a proliferation culture medium, a strong seedling culture medium or a rooting culture medium is injected into the culture bag, so that anoectochilus formosanus explants are subjected to tissue culture and seedling culture. When the anoectochilus formosanus is subjected to the tissue culture by adopting the method, the pollution can be effectively controlled, and the contamination rate is smaller than 3%; an inoculation progress is simplified and damages to the seedlings are reduced; an inoculating speed is improved and anoectochilus formosanus tissue culture seedlings with strong disease resistance are obtained; and meanwhile, the operation of taking out seedlings from the culture bottle after bagged tissue culture also can be avoided, and integration of tissue culture, seedling growing and product transportation of the anoectochilus formosanus is realized, so that the production cost is greatly reduced, the yield is improved and the growth period is shortened.

Description

technical field [0001] The invention belongs to the technical field of planting and cultivation of traditional Chinese medicinal materials, and in particular relates to a method for cultivating Aureus aureus with a cultivation bag. Background technique [0002] Anoectochilus roxburghii (Wall.) Lindl. is a rare medicinal plant of Orchidaceae, also known as Anoectochilus roxburghii (Wall.) Lindl. It is mainly distributed in Fujian and other southern provinces of China. The whole herb is used as medicine. Its nature is flat and sweet, and it has the effects of clearing heat, cooling blood, dehumidification and detoxification. It has the reputation of "King of Medicine" and "Golden Grass" among the people, and it has many aspects such as ornamental value, making it It has broad development prospects. [0003] At present, the artificial cultivation of clematis mainly adopts glass culture bottles to carry out group cultivation of seedlings. However, glass culture bottles are fra...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 饶宝蓉陈泳和黄小云吴勇军陈敏健
Owner NANPING HUAKE BIOTECH CO LTD
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