Anoectochilus formosanus Hayata artificial seed preparation method

A technology of artificial seeds and production methods, applied in horticultural methods, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of high cost, long production cycle of test-tube seedlings, low survival rate of hardened seedlings and transplanting, and achieve Overcoming the low natural reproduction rate and making up for the effect of a long production cycle

Inactive Publication Date: 2006-10-11
NANJING UNIV
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Problems solved by technology

In recent years, although many researchers have made great breakthroughs in tissue culture and in vitro rapid propagation of clematis, the production cycle of test-tube seedlings is long, the cost is high, the survival rate of hardening and transplanting is low, and it is inconvenient. storage and transportation, thus limiting large-scale production

Method used

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  • Anoectochilus formosanus Hayata artificial seed preparation method

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Embodiment Construction

[0019] 1. Induction and proliferation culture of protocorms of Aurantia clematis

[0020] (1) Induction of the protocorm of Aurea clematis

[0021] Rinse the fresh wild clematis whole herb with tap water for 1-2 hours, then shake it with soapy water for 3-5 minutes, wash off the soapy water, rinse it once with sterile water, soak it in 75% ethanol for 0.5-1 minute, and then soak it in 0.1% ethanol HgCl 2 Soak in the solution for 10-15 minutes, rinse with sterile water 3-5 times, blot the surface moisture, remove the roots and leaves, cut into about 1 cm long sectioned stems, and store them aseptically for later use. In a sterile environment, inoculate the sterilized clematis ligamentum stems on the protocorm induction medium, and the medium composition is MS+2.0~3.0mg·l -1 6-BA+0.2~0.5mg·l -1 NAA+0.5~0.8mg·l -1 ZT+25.0~30.0g·l -1 Sucrose+6.0~8.0g·l -1 Agar, pH 5.7-6.0. After culturing for 25-30 days under the conditions of 25±1°C, 500-1000lx low-light irradiation for 10-...

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Abstract

The invention belongs to field of artificial crop seed production technology, and provides a key technology for producing artificial seed of gold thread lotus which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of gold thresd lotus and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

Description

1. Technical field [0001] The invention belongs to the technical field of plant artificial seed production. 2. Background technology [0002] Anoectochilus roxburghii (Wall.) Lindl. is a perennial herbaceous plant of the genus Anoectochilus in the family Orchidaceae, mainly distributed in Japan, China, Sri Lanka, India and Nepal in Asia. Aurantia clematis is a traditional precious medicinal material in my country. The whole herb is used as medicine. It tastes sweet and slightly bitter, and its nature is flat and slightly cold. It has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain. Convulsions, venomous snake bites, venereal diseases, rheumatism, tumors, etc. (Journal of Jiangxi University of Traditional Chinese Medicine. 1999, 11: 188-189; Chinese Herbal Medicine. 2000, 31: 944-946), known as "Golden Grass", "God "Medicine", "Medicine Tiger", "Medicine King" and other laudatory titles. Clematis clematis has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H5/10C12N5/04
Inventor 张明生王勐骋甄艳杨永华曹巧云殷召静王康
Owner NANJING UNIV
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