84 results about "Venomous snake" patented technology

The invention discloses application of Chinese crinums to purification of livestock and poultry breeding biogas liquid, belonging to the field of wastewater treatment. The problem of pollution caused by the livestock and poultry breeding biogas liquid is permanently solved by planting the Chinese crinums in environments which are polluted by the livestock and poultry breeding biogas liquid, and by fully utilizing the characteristics that the Chinese crinums have an absorbing effect to pollutants such as NH4<+>-N, TN, TP and CODcr (Chemical Oxygen Demand cr) in the livestock and poultry breeding biogas liquid, have strong vitality, can be directly planted, are not needed to be specially managed and are perennial. The investment is small, the operating cost is low, the effect is good, the operation is simple and feasible, and the purification effect of an artificial wetland system in winter can be guaranteed. The harvested Chinese crinums can be used for clearing away heat and toxic materials, removing stasis, relieving pains and treating heat sores, lymphnoditis, sphagitis, bone fracture, venomous snake bites and the like. By using the Chinese crinums to treat wastewater and by combining environmental protection with landscape construction and economic construction, the characteristic of combining theoretical researches with practical application is achieved.

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

The invention belongs to the technical field of determination of biological immune methods, and particularly relates to a detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and a preparation method and application thereof. The detection card is composed of a PVC backboard, a nitrocellulose membrane (NC membrane), a colloidal gold pad, a sample pad and water absorption filter paper. The sample pad is made of glass fibers. A colloidal gold labeled rabbit anti-beta-BGT polyclonal antibody is sprayed on the colloidal gold pad. The C line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG polyclonal antibody, and the T line of the nitrocellulose membrane is coated with a rabbit anti-beta-BGT polyclonal antibody. The preparation method is simple, the specificity is high, the stability is good, the detection method is simple, carrying is convenient, bungarotoxin can be rapidly detected, whether a person is bitten by bungarus multicinctus or not can be judged within 5-10 minutes, a diagnostic tool for judging venomous snake bites can be provided for snake bite treatment, more treatment time can be gained for patients, and the detection card can assist in the clinical anti-snake venom serum dosage, and is economical and practical.

A poisonous snake feeding fodder for improving snake venom yield is obtained by mixing and triturating the following substances by mass: 20 parts of eggs, 20 parts of lean pork, 10 parts of chicken, 15 parts of fresh fish, 15 parts of fresh earthworm, 10 parts of mouse meat, 7 parts of cockroaches, 0.2 part of rare earth elements, and 0.02 part of caffeine. The poisonous snake feeding fodder for improving snake venom yield adopts fresh animal meat and insects as protein and energy components, is added with additives for weight increasing and growth promotion, is also added with a component of vitamin C which is beneficial to snake venom synthesis, is further added with caffeine for improving snake exercise amount, and thus greatly increases the yield of snake venom.

The invention relates to a blood-activating and pain-relieving plaster, and a preparation method thereof. A formulation of the plaster comprises: garter snake, snake slough, nux vomica, doubleteeth angelica root, rheum, gansui root, scorpion, rehmannia dride rhizome, asurum uropeum, zhishi, fructus crotonis, almond, divaricate saposhnikovia, kusnezoff monkshood root, angelica archang lica, nutgrass galingale rhizome, clam powder, frankincense, pollen, whole ginseng, whole angelica, myrrh, knoxia root, herba ephedrae, dragon's blood, large cenpitede and forest musk. The preparation method comprises the following steps: pouring sesame oil or perilla oil to a container, boiling the oil, then adding garter snake, snake slough, nux vomica, doubleteeth angelica root, rheum, gansui root, scorpion, then adding other herbs, adding the dragon's blood after 4-5 hours; carrying out decocting with mild fire after all the herbs are added to the container; placing the decocted medical solution in cold water filled in a container to carry out cooling until the solution is transformed into the black solid. According to the present invention, the plaster can be directly pasted on the affected part of the human body and is applicable for most people; the plaster has characteristics of no skin irritation, no toxic and side-effect, simple process, fast release rate, quick therapeutic effect; the plaster is applicable for treating snake bite, small area burn, scald and other surgical wounds, and has effects of blood activating and pain relieving.

Disclosed is a bionic snake feeding device. The bionic snake feeding device comprises a box for the captivity of snakes, one side of the box is provided with a feeding part for feeding the snakes in the box, the box is of a hollow cuboid, the direction of the length of the box is defined as an x axis, the direction of the width of the box is defined as a y axis, and the direction of the height of the box is defined as a z axis; the feeding part comprises a base and a food piecing pipe for piecing food and sending the food into the box, the food piecing pipe extends in the y-axis direction, the inner end of the food piecing pipe is located in the box, and the outer end of the food piecing pipe is located out of the box; the outer end of the food piecing pipe is connected with a driving device for driving the inner end of the food piecing pipe to make reciprocating motion in the x-axis direction in the box, and the driving device is arranged on the base; a guide section capable of glowing and heating is coaxially connected to the inner end of the food piecing pipe to instruct the snakes to seek food; the bionic snake feeding device further comprises a controller for controlling the feeding part and the guide section to work, and the controller is connected with the feeding part and the guide section separately. Through the adoption of the bionic snake feeding device, the time, labor and efforts are saved in the snake feeding process, the snake feeding effect is good, the venomous snake bites can be prevented, and the device is safe enough.

The invention relates to an emergency medicine for snake wounds. The medicine comprises the following components in parts by weight: 5-12 parts of Jacaranda flower root, 5-12 parts of Ainsliaea fragrans, 3-5 parts of radix angelicae, 5-10 parts of common andrographis Herb, 1-4 parts of Paris polyphylla, 5-10 parts of Circium japonicum root, and 7-12 parts of Chinese lobelia. The preparation method comprises the following steps of: 1) crushing Jacaranda flower root, Paris polyphylla and radix angelica and uniformly mixing to obtain fine powder; 2) adding water to Circium japonicum root, Ainsliaea fragrans, common andrographis Herb and Chinese lobelia, and decocting concentrating the filtrate to obtain clear paste; 3) adding Jacaranda flower root, Paris polyphylla and radix angelica fine powder into the clear paste filtrate and uniformly mixing; and 4) drying, crushing to obtain fine power to obtain the medicine. Through clinical use for many years, the medicine provided by the invention is an emergency and treatment medicine for snake wounds which has remarkable curative effect and no adverse effect, is convenient to take and store and low in cost. The medicine is used for treating various venomous snake bites and has the functions of clearing away heat, detoxifying, stopping bleeding, removing swelling and relieving pain.

A phospholipase A2 inhibitor protein designated “Phospholipase Inhibitor from Python” (PIP)—formerly designated “Python Antitoxic Factor” (PAF)—is given by SEQ ID NO:2. The partial amino acid sequence for PIP was initially determined from the native protein purified from the blood serum of a non-venomous snake, Python reticulatus. The complete PIP polynucleotide sequence was obtained from a cDNA clone encoding PIP, given by SEQ ID NO:1, along with the full amino acid sequence deduced from it. Also disclosed is a recombinant protein PIP, which shows strong lethal toxin neutralizing activity similar to the native PIP, and has potent anti-inflammatory activity. Both the native and the functionally equivalent recombinant PIP are useful for the prevention or treatment of conditions such as snakebites, insect stings, and inflammatory diseases. Also, phospholipase A2 (PLA2) inhibitory polypeptides designated P-0029, P-0009, and P-0006, the sequences of which are given as SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, respectively, are disclosed. Those polypeptides, and their synthetic chemical analogues and polypeptide variants that inhibit PLA2 activity and alleviate inflammation, may also be used in the diagnosis, study, prevention, and treatment of PLA2-related human inflammatory diseases.

The invention relates to anti-venomous-snake PLA2 protein antibodies and application thereof. The anti-venomous-snake PLA2 protein antibodies comprise monoclonal antibodies of tritoxomab-PLA2, protoxomab-PLA2, viptoxomab-PLA2, najatoxomab-PLA2 or glotoxomab-PLA2, and have high affinity and specificity. The monoclonal antibodies are prepared by adopting PLA2 proteins of the five kinds of venomous snakes as antigens for immunizing mice through a hybridoma fusion technology, antibody screening is conducted by taking the amino acid sequences of of two hypervariable regions in the PLA2 proteins asantigens, and finally, ten monoclonal antibodies of tritoxomab-PLA2, protoxomab-PLA2, viptoxomab-PLA2, najatoxomab-PLA2 and glotoxomab-PLA2 are obtained in total. One or a combination of more of the ten monoclonal antibodies can be used as a diagnostic test reagent for snake wounds. The anti-venomous-snake PLA2 protein antibodies can be prepared into the diagnostic reagent or kit for the snake wounds, and the diagnostic efficiency of the snake wounds is improved.

The invention relates to picking equipment for medical care Chinese medicinal materials. The picking equipment comprises a bottom plate, a collecting device is installed on the bottom plate, lifting air cylinders are evenly arranged in the circumferential direction of the lower end of the bottom plate, the bottom ends of the lifting air cylinders are installed on an annular picking plate through flanges, a picking execution device is installed on the annular picking plate, flexible skirts are evenly arranged in the circumferential direction of the lower end of the bottom plate, and the lower ends of the flexible skirts are installed on the picking execution device. The picking equipment can solve the problems that in the prior art, in the process of picking ganoderma lucidum, the ganodermalucidum needs to be picked and collected manually, human bodies are prone to being hurt because people need to repeatedly stoop, squat and rise during manual picking of the ganoderma lucidum, the ganoderma lucidum is prone to being damaged by negligence under long-term, repeated, complex and heavy working operation and easily attracts poisonous insects, venomous snakes and other creatures, and people is prone to being attacked by parasitic poisons when manually picking the ganoderma lucidum.

The invention discloses a method for identifying a vipera russelli venom. The method comprises the following steps of (1) dissolving vipera russelli venom toxin lyophilized powder to prepare a proteinsolution; (2) performing SDS-PAGE electrophoresis analysis; (3) conducting LC-MS / MS identification. In addition, the invention discloses application of the method for identifying the vipera russellivenom in preparing anti-viper-venom serum for treating viper venom poisoning. By means of the method, laboratory identification can be conducted on the viper venom to more accurately control the quality of the viper venom; it can be ensured that the special-effect anti-viper-venom serum for treating viper venom poisoning is safe, effective and controllable in quality, and the method has very important significance in the aspects of viper species identification, viper wound diagnosis and treatment, application of other viper venom and the like. The method is high in detection sensitivity and correct detection rate and simple in operation process and has no cross reaction.

The invention relates to a snake venom taking device which is mainly composed of a handle, a silica gel shell, a cold storage chamber and a battery. An electric heating wire connected with the battery is arranged in the silica gel shell, and the silica gel shell is communicated with the cold storage chamber. A use method mainly includes the steps of firstly, rubbing the silica gel shell with an animal so that the silica gel shell can be stained with the smell of the animal; secondly, turning on a power supply switch so that the electric heating wire can be powered on for heating and the silica gel shell can be heated; thirdly, holding the handle by hand to make the silica gel shell get close to and irritate a venomous snake, inducing the venomous snake to bite the silica gel shell and inject in venom, and making the venom flow into the cold storage cavity. The device and the method have the advantages that safety is achieved because the snake does not need to be caught for venom taking, the venom taking efficiency is high, operation is quite easy, the snake is not injured, the structure is simple, and the manufacturing cost is low.

The invention discloses a novel external medicament for treating diseases such as fester and necrosis of wounds caused by venomous snake bites, fester of wound surface caused by burns and scalds, necrosis periostitis and osteomyelitis. The external medicament is a formula taking nine Chinese medicinal herbs as raw materials. The external medicament has the advantages of scientific and unique formula, and obvious medicinal efficacy and curative effect.

The invention discloses a method for preparing an antivenom oral liquid with tripterospermum cordatum as the major raw material. The method comprises the following steps of: (1) treatment of the tripterospermum cordatum raw medicinal material: performing vacuum drying, crushing and enzymolysis on the raw medicinal material; (2) extraction of active ingredients of tripterospermum cordatum: performing twice ultrasonic extraction with water as a solvent, blending the filtrates and then performing alcohol precipitation to remove impurities; and (3) preparation of the antivenom oral liquid: after freezing and filtering, filtering the mixed filtrate by using a bacterial filter in a sterile area and then filling the filtered liquid. The antivenom oral liquid obtained by the method disclosed by the invention has tremendous curative effect on neural venoms such as the snake venom of coral snake and the like, and blood circulation venoms and mixed venoms and snake wounds caused by long-noded pit viper, cobra and the like; and the effect of the antivenom oral liquid is superior to that of the traditional medication ways such as taking orally after decocting with water and dressing the wound after chewing. The method is convenient for industrialized, large-scale and standardized production, and wide in market prospect.

The invention relates to the technical field of traditional Chinese medicines and in particular relates to anti-inflammatory active ingredients of a Hainan dischidia chinensis water extract. The active ingredients are obtained through the following steps: soaking dischidia chinensis in water overnight, carrying out ultrasonic-assisted extraction, decoction and extraction, mixing filtrates and carrying out vacuum concentration to obtain a water extract; adding water to suspend the water extract to obtain suspension and extracting the suspension with ethyl acetate, n-butyl alcohol, petroleum ether and a solvent mixture in sequence, thus obtaining various extracts respectively. The anti-inflammatory active ingredients have the beneficial effects that the anti-inflammatory active ingredients are simple in process and reasonable in operation; meanwhile, anti-inflammatory effective parts of the dischidia chinensis alcohol extract can be obtained, which is a new discovery in the anti-inflammatory effect of the natural product, Hainan dischidia chinensis; theoretical and experimental bases are provided for clinical application of dischidia chinensis to treatment of expectoration due to lung dryness, hemoptysis, pertussis, infantile malnutrition, dysentery, toxins and pus of scabies, pain and swelling from injuries from falls and venomous snake bites.

The invention discloses reptilase sample decorated by polyethylene glycol. Its formula is mPEG-O-(CH2)n-T-NH-R whose base group definition can be seen in the specification. It can be used as anticoagulation thrombolysis medicine or nerve protection medicine. And it has lower immunogenicity than undecorated enzyme, higher enzyme activity and fiber reducing capability than polyethylene glycol decoration fiber reducing enzyme. Its preparation method is simple and easy.

The invention relates to the technical field of traditional Chinese medicine, in particular to an anti-inflammatory active ingredient from Dischidia chinensis alcohol extract. The ingredient is prepared by: sieving Dischidia chinensis powder with a 40-mesh sieve, soaking the sieved powder in ethyl alcohol overnight, performing ultrasonic-assisted extraction, combining filtrates to obtain extract, adding water to dissolve the extract, eluting dissolved extract with ethyl alcohol, collecting 60-90% ethyl alcohol eluate, separating the eluate with D941 chromatographic columns, performing eluting with 95% ethyl alcohol, collecting eluate, and subjecting the eluate to vacuum concentration to obtain ethanol extract; adding water into the ethanol extract for suspending, and performing successive extraction with solvents. The ingredient is simple and reasonable; the anti-inflammatory active ingredient from Dischidia chinensis can be obtained and belongs to a new discovery in anti-inflammatory action of Dischidia chinensis being a natural product; certain experimental basis is provided for Dischidia chinensis in terms of nongovernmental medication for treating lung dryness and expectoration, hemoptysis, pertussis, infantile malnutrition, dysentery, scabies with pus and toxin, traumatic injuries, swelling and pain, and venomous snake bites.

The invention relates to a medicine for external application for treating snake bite, which is prepared from Chinese lobelia, impatiens balsamina, coptis chinensis, subprostrate sophora, acanthopanax root, folium isatidis, rehmannia root and golden cypress in parts by weight. The medicine for external application for treating snake bite has the effects of clearing away heat and toxic materials and relieving pain and swelling. Through observation under an electronic microscope, the medicine can directly destroy and decompose enzyme proteins of snake venom to enable the enzyme proteins to lose toxicity, has excellent effect on treating venomous snake bite, and has no toxic or side effect.

The invention discloses medicinal wine for treating snake and insect bites and stings. The medicinal wine comprises, by weight, 35-45 g of herba scutellariae barbatae, 35-45 g of chenopodium ambrosioides, 25-35 g of solidago decurrens, 25-35 g of radix zanthoxyli, 25-35 g of radix cynanchi paniculati, 20-30 g of murraya paniculata, 20-30 g of cavalerie mosla herb, 15-25 g of herba asari, 15-25 g of Chinese lobelia, 15-25 g of angelica dahurica, 10-20 g of cortex phellodendri, 10-20 g of radix et rhizoma rhei, 10-20 g of realgar 10-20 g of radix clematidis, 10-20 g of fructus evodiae, 10-20 g of faeces trogopterpri, 5-15 g of rhizoma coptidis and 5-15 g of radix cyclea hypoglauca. The invention also discloses a preparation method of the medicinal wine for treating the snake and insect bitesand stings. The medicinal wine prepared by the method for treating the snake and insect bites and stings is adopted for external use, can clear away heat, achieve detoxification, promote circulationof qi and relieve pain, can be used for treating bites and stings caused by snakes, cockroaches, mosquitoes and the like, and has a remarkably curative and quick effect.

The invention discloses a method for preparing a medicament for treating a venomous snake bite. The method is implemented by a technical scheme which comprises: putting raw materials of clematis root, weeping forsythia and pinellia tuber between two tiles; closing and placing the two tiles into a drying box; heating for 20 to 30 minutes at the temperature of between 100 and 120 DEG C; taking the raw materials out and decocting and boiling the raw materials for 15 minutes; filtering to obtain decoction; adding water again and decocting and boiling the raw materials for 20 minutes; removing dregs; and uniformly blending decoction obtained at two times to obtain the medicament for treating the venomous snake bite, wherein the medicament is taken by several times. Simultaneously, realgar and trogopterus dung are ground into powder together, and the powder is applied onto wounds after being mixed with good wine. The method can improve the curative effect obviously.

A Chinese medicine for dropping drug and treating the viper bite and poisoning is prepared from 6 Chinese-medicinal materials including barbat skullcap, mockstrawberry herb, polygala root, etc. Its advantages are high curative effect, no dependency, and short course of treatment (7 days).

A traditional Chinese medicine preparation for treating venomous snake bites, which is divided into internal medicine and external medicine, wherein: the internal medicine is made of the following components by weight: 10-25 grams of Zhonglou, 10 grams of melon seed gold, and 20 grams of snakeberry , Commelina 0‑15 grams, Lobelia 45‑50 grams, Scutellaria barbata 45‑50 grams; the medicine for external application is made of the following components by weight: Polyphylla 10‑25 grams, Lobelia 45‑50 grams, Scutellaria barbata 45‑50 grams, rhubarb 10 grams, cork 10 grams, knotweed 10 grams, viola 10 grams. Treatment method: first clean the wound with medicated wine prepared by external application of medicine, and then take out the poison sac from the bitten place with a needle. Take decoction or pills decocted with internal medicine, and then use external medicine to clean the wound; internal medicine can protect the internal organs from the invasion of snake venom.

A universal detection system based on allosteric aptamers, signal amplification cascade, and eye-detectable phrase transition. A broadly applicable homogeneous detection system is provided. It utilizes components of the blood coagulation cascade in the presence of polystyrene microspheres (MS) as a signal amplifier. Russell's viper venom factor X activator (RVV-X) triggers the cascade, which results in an eye-visible phase transition—precipitation of MS bound to clotted fibrin. An allosteric RNA aptamer, RNA132, with affinity for RVV-X and human vascular endothelial growth factor (VEGF165) was created. RNA132 inhibits enzymatic activity of RVV-X. The effector molecule, VEGF165, reverses the inhibitory activity of RNA132 on RVV-X and restores its enzymatic activity, thus triggering the cascade and enabling the phase transition. Similar results were obtained for another allosteric aptamer modulated by a protein tyrosine phosphatase. The assay is instrumentation-free for both processing and readout.

The invention discloses an anion far infrared anti-electromagnetic wave selenium-germanium element and traditional Chinese medicine nanometer snake and misgurnus anguillicaudatus powder capsule and belongs to the technical field of medical-care capsule production. Various snake medicinal materials and misgurnus anguillicaudatus which is honored as ginseng in water respectively contain rich macroelements and microelements, especially ferrum, zinc, manganese and copper are high in content; and proteins especially in hagworm flesh contain metallothionein, and the metallothionein is an in vivo free-radical scavenger which is highest in capacity of scavenging in vivo free radicals so far. The capsule disclosed by the invention comprises a mixture of nanometer dry powder of the flesh tissues of venomous snakes or non-venomous snakes including land snakes, ocean snakes and desert snakes, nanometer dry powder of the flesh tissues of the misgurnus anguillicaudatus, microelement selenium or zinc or germanium nanometer dry powder, bee-series nanometer dry powder, medical stone, tourmaline and a macroelement / microelement enhancer, wherein the medical stone and tourmaline can release various elements to regulate, supplement and oppose in vivo element balance and can spontaneously generate anion far infrared anti-electromagnetic waves; the superfine powder treated by using a nanometer particle size superfine micronization device is sieved by 800-1200 meshes to obtain nanometer particles; and the nanometer particles are 0.5-5mu.m in medium particle diameter. The capsule prepared from the nanometer dry powder can be used for effectively enhancing tissue cell activity, organism immunity and disease resistance and is capable of building body and prolonging life.

The Chinese medicine adhesive plaster-Wudugao plaster for curing furunculosis, malignant boil, scrofula, suppurative lymphatic abscess and suppurative fistula, etc. is made up by adopting the following steps: placing the Chinese medicinal materials of poisonous snake, Chinese wolfsbane root, scorpion, momordica seed and centipede into boiling agrimony seed oil, deep-frying them to form charcoal, taking out and filtering, placing the minium and boiling to obtain paste, so that it can be used for making the invented adhesive plaster for external application.

The invention relates to an emergency treatment device for venomous snake bite. The prior art lacks an effective venom elimination tool or venom elimination medicament for all snake venoms. Therefore, the emergency treatment device for venomous snake bite comprises a metal tooth body, wherein the metal tooth body is a combustible or is provided with the combustible which can be rapidly combusted to release heat when encountering open fire or water, and is a magnesium rod which is the combustible and is wrapped with an oxygen isolation protective film; or the combustible is a metal sodium particle which is embedded into the tip end part or the front side of the metal tooth body and is sleeved with a sealing protective sleeve; or the metal tooth body is shaped like a bent sharp cone and is made from copper or aluminum, a pit is formed in the root of the metal tooth body, and the combustible is gunpowder which is arranged in the pit. The emergent treatment device has the advantages of simple structure and convenience in carrying; when the emergent treatment device is used, a user is probably locally burnt, but snake venom can be instantaneously eliminated, and the emergency treatment device has miraculous effects on various snake venoms, and is applied to the emergency treatment of bite of various venomous snakes.

The invention provides a traditional Chinese medicinal composition. The traditional Chinese medicinal composition comprises, by weight, 20-50 parts of Chinese lobelia, 20-50 parts of Paris polyphylla,20-50 parts of oldenlandia diffusa, 20-50 parts of rheum officinale, 20-50 parts of jasmines, 20-50 parts of caulis lonicerae, 20-50 parts of earthworms, 20-50 parts of red peony roots, 20-50 parts of pseudo-ginseng, 20-50 parts of peach kernels, 20-50 parts of red flowers, 20-50 parts of radix achyranthis bidentatae and 20-50 parts of ligusticum wallichii. The invention further provides a preparation method and application of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition is cheap, efficient and convenient, and can obviously relieve swelling andpain generated by the poisonous snake bite to avoid surgical drainage and amputation risks.

The invention discloses high-purity anti-snake-venom serum and a preparation method thereof. The high-purity anti-snake-venom serum is obtained mainly by the steps of activating a polysaccharide carrier by cyanogen bromide, coupling a toxic snake toxin to the cyanogen bromide activated polysaccharide carrier and purifying anti-snake-venom serum F (ab') 2 through an affinity chromatography method.The anti-snake-venom serum product with high neutralizing potency obtained by the preparation method has a good function of neutralizing snake venom, the amount of proteins without neutralizing toxiceffects in the product is greatly reduced, the occurrence rate of allergic reactions caused by heterogeneous proteins can be obviously reduced, and the severity of the allergic reactions can be reduced. In addition, the preparation method is simple, mild in condition and suitable for large-scale popularization and application.

The invention discloses a snake venom collecting device based on mechanical arm clamping. The snake venom collecting device comprises a box body, an arrangement cavity is formed in the upper side of the box body, a clamping mechanism is arranged on the upper side of the arrangement cavity, the clamping mechanism comprises a first sliding rail, the first sliding rail is fixed to the inner wall of the upper side of the arrangement cavity, a first sliding groove with a downward opening is formed in the first sliding rail, a first sliding block is arranged in the first sliding groove in a slidingmanner, a first threaded rod is arranged in the first sliding groove in a rotating manner, the first sliding block is in threaded connection with the first threaded rod, and a first connecting piece is fixedly arranged on the lower end face of the first sliding block. According to the snake venom collecting device, snake venom collecting work can be automatically completed, phototaxis and aversionto high temperature of part of poisonous snakes are utilized to enable the poisonous snakes in a cavity to directionally emerge out through a hole, a mechanical arm is arranged outside the hole to clamp the heads and necks of the snakes, a collecting mechanism is additionally arranged to induce the poisonous snakes to bite a collecting box, a thin film is arranged above the collecting box, and poisonous teeth pierce the thin film to inject venom into a collecting cavity.