Anti-inflammatory active ingredient from Dischidia chinensis alcohol extract and application thereof

An active ingredient, the technology of raspberry alcohol, which is applied in the field of traditional Chinese medicine, can solve the problems of insufficient development and application of raspberry, achieve good development prospects, relieve early symptoms, and have low toxicity effects

Inactive Publication Date: 2015-12-02
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above insufficient problems in the development and application of saline tree lotus, the present invention provides an anti-inflammatory active ingredient of saline tree lotus alcohol extract

Method used

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  • Anti-inflammatory active ingredient from Dischidia chinensis alcohol extract and application thereof
  • Anti-inflammatory active ingredient from Dischidia chinensis alcohol extract and application thereof
  • Anti-inflammatory active ingredient from Dischidia chinensis alcohol extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: water extraction

[0025] 1 material

[0026] 1.1 Drugs and reagents Dischidiachinensis Champ was collected from the crater of Haikou City, Hainan Province, and was identified by Dai Haofu, a researcher at the Institute of Biology, Chinese Academy of Tropical Agricultural Sciences. .exBenth) whole plant. Aspirin enteric-coated tablets (Jiangsu Pingguang Pharmaceutical Co., Ltd., batch number: 140801); Evans blue (BS, 5g, Aladdin Reagent Shanghai Co., Ltd.); 0.9% sodium chloride injection (pharmaceutical grade, Hunan Jinjian Pharmaceutical Industry limited liability company); carboxymethylcellulose (AR, 500g / bottle, Sinopharm Chemical Reagent Factory); xylene (AR, 500mL / bottle, Tianjin Damao Pharmaceutical Chemical Reagent Factory); n-butanol, ethyl acetate and Petroleum ether was analytically pure.

[0027] 1.2 Animals Kunming mice, clean grade, weighing 18-22 g, provided by Changsha Tianqin Biotechnology Co., Ltd., experimental animal use license numbe...

Embodiment 2

[0055] Take 5kg of dry Sapia praecox, crush it and pass it through a 40-mesh sieve, soak it in 30 times the amount of water overnight, then extract it with ultrasonic waves for 2 hours, the ultrasonic frequency is 40-50KHz, and the temperature is 70-80°C. After ultrasonic extraction for 1.5 hours, turn on at the same time Decoct in water for a total of 30 minutes, repeat the above-mentioned soaking, ultrasonication, and decoction 3 times, cool, filter, combine the filtrates, and concentrate under reduced pressure to an extract to obtain 229.5 g of water extract with a recovery rate of 4.59%. Take an appropriate amount of water extract and add 10 times of water to suspend to obtain a suspension, which is sequentially extracted with ethyl acetate, n-butanol, and petroleum ether. The volume ratios of ethyl acetate, n-butanol, petroleum ether and the suspension are 1:1, respectively. The extraction time was 10 min, respectively, and concentrated under reduced pressure to obtain eth...

Embodiment 3

[0057] Take 5kg of dry Sapia praecox, crush it and pass it through a 40-mesh sieve, soak it in 30 times the amount of water overnight, then extract it with ultrasonic waves for 2 hours, the ultrasonic frequency is 40-50KHz, and the temperature is 70-80°C. After ultrasonic extraction for 1.5 hours, turn on at the same time Decocting for a total of 30 minutes, repeating the above-mentioned soaking, ultrasonication, and decocting 3 times, cooling, filtering, combining the filtrates, and concentrating under reduced pressure to an extract form to obtain 229.5 g of water extract with a recovery rate of 4.59%. Take an appropriate amount of water extract and add 8 times of water to suspend to obtain a suspension, and then extract it with ethyl acetate, n-butanol, and petroleum ether. The volume ratios of ethyl acetate, n-butanol, petroleum ether and the suspension are 1:2, respectively. The extraction time was 6 minutes respectively, and concentrated under reduced pressure to obtain et...

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Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to an anti-inflammatory active ingredient from Dischidia chinensis alcohol extract. The ingredient is prepared by: sieving Dischidia chinensis powder with a 40-mesh sieve, soaking the sieved powder in ethyl alcohol overnight, performing ultrasonic-assisted extraction, combining filtrates to obtain extract, adding water to dissolve the extract, eluting dissolved extract with ethyl alcohol, collecting 60-90% ethyl alcohol eluate, separating the eluate with D941 chromatographic columns, performing eluting with 95% ethyl alcohol, collecting eluate, and subjecting the eluate to vacuum concentration to obtain ethanol extract; adding water into the ethanol extract for suspending, and performing successive extraction with solvents. The ingredient is simple and reasonable; the anti-inflammatory active ingredient from Dischidia chinensis can be obtained and belongs to a new discovery in anti-inflammatory action of Dischidia chinensis being a natural product; certain experimental basis is provided for Dischidia chinensis in terms of nongovernmental medication for treating lung dryness and expectoration, hemoptysis, pertussis, infantile malnutrition, dysentery, scabies with pus and toxin, traumatic injuries, swelling and pain, and venomous snake bites.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to an anti-inflammatory active ingredient of an alcohol extract of saline tree lotus, and also relates to the application of the anti-inflammatory active ingredient of an alcohol extract of tree lotus hainan. Background technique [0002] Dischidia chinensis (scientific name: Dischidia chinensis) is an epiphytic vine of the genus Dischidia chinensis in the Radixaceae family. It is a unique plant in China. In my country, it is mainly distributed in Hainan, Guangdong, Guangxi and other provinces and regions. In Hainan, it is mainly produced in Ledong, Sanya, Baoting and other places, and grows in mountainous forest valleys or open fields at low to middle altitudes. The whole plant of the lotus eye tree is used as medicine, which has the effect of clearing the lungs and reducing phlegm, cooling blood and detoxification. It is used in the folk to treat dryness of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P29/00
CPCY02A50/30
Inventor 余邦良杨柳王小蒙张军于春伟姚瑰玮陈湛娟
Owner HAINAN MEDICAL COLLEGE
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