Method for identifying vipera russelli venom and application of method

An identification method for Viper rotundum, which is applied to the identification of types of snake venom by using snake venom protein analysis technology. The application of this identification method, in the field of identification of Viper rotundus venom, can solve the complicated and cumbersome operation process and low detection sensitivity , low correct detection rate and other issues, to achieve the effect of simple operation process, high detection sensitivity and high correct detection rate
CN110426522AActive Publication Date: 2019-11-08SHANGHAI SERUM BIOTECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI SERUM BIOTECH
Publication Date
2019-11-08

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Abstract

The invention discloses a method for identifying a vipera russelli venom. The method comprises the following steps of (1) dissolving vipera russelli venom toxin lyophilized powder to prepare a proteinsolution; (2) performing SDS-PAGE electrophoresis analysis; (3) conducting LC-MS / MS identification. In addition, the invention discloses application of the method for identifying the vipera russellivenom in preparing anti-viper-venom serum for treating viper venom poisoning. By means of the method, laboratory identification can be conducted on the viper venom to more accurately control the quality of the viper venom; it can be ensured that the special-effect anti-viper-venom serum for treating viper venom poisoning is safe, effective and controllable in quality, and the method has very important significance in the aspects of viper species identification, viper wound diagnosis and treatment, application of other viper venom and the like. The method is high in detection sensitivity and correct detection rate and simple in operation process and has no cross reaction.
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Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for identifying the venom of Viper viper, in particular to a method for identifying the type of snake venom using snake venom protein analysis technology; in addition, the invention also relates to the application of the identification method, in the anti-venom The production of serum, the identification of venomous snake species, the diagnosis and treatment of snakebite and the application of other snake venoms are of great significance. Background technique

[0002] Venomous snakebites are common in subtropical and tropical regions. In the rural areas of Asia, Africa, Latin America and Oceania, venomous snakebite is a particularly prominent public health problem, and the incidence of snakebite in my country shows a trend of increasing year by year.

[0003] Snake venom is mostly light yellow or milky white translucent viscous liquid, with more than 100 components. Eac...

Claims

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