44 results about "Venom Protein" patented technology

The invention discloses a method for regulating and controlling synthesis of ectoine in escherichia coli, and relates to the technical field of biological medicines. According to the method for regulating and controlling the synthesis of the ectoine in the escherichia coli, disclosed by the invention, a gene segment of a tisB toxic protein is used for constructing a tisB toxic protein expression vector pET28a (+)-p2-22-tisB, and the tisB toxic protein expression vector is transferred into an escherichia coli engineering bacterium for synthesizing the ectoine, so that the escherichia coli engineering bacterium E.coli Ect pET28a (+)-p2-22-tisB for spontaneously inhibiting the synthesis of the ectoine is obtained, and meanwhile, the normal synthesis of the ectoine at an early stage can be ensured. The nucleotide sequence of the gene segment of the tisB toxic protein is as shown in SEQ ID NO. 1. In the first three rounds of fermentation, the yield of ectoine is close to that of the control engineering bacteria; after the seventh round, the yield of ectoine is sharply reduced to 2.575 g / L, and during the twentieth round of fermentation, the yield of ectoine is zero.

The invention provides a predatory mite venom protein or a spider venom protein. The amino acid sequences of the predatory mite venom protein and the spider venom protein are selected from SEQ ID NO.1-8 or any one of SEQ ID NO.1-8 with 95% or more of identity. Tetranychus urticae tests show that after spraying treatment, the predatory mite poison protein or spider poison protein provided by the invention achieves a 100% lethal effect within 48 hours, and is a known protein pesticide with the highest tetranychus urticae lethal rate at present. The predatory mite poison protein or spider poison protein provided by the invention has high specificity to spider mites, is mild and harmless to human bodies and other organisms in the environment, can be used in farmlands, gardens and even family potted plants, and can be naturally degraded; the production process is simple and efficient, and has the potential of large-scale application.

The invention relates to the field of biological information, and discloses a toxic protein library building method, device, equipment and medium, and the method comprises the following steps: obtaining a consensus peptide structure corresponding to a confirmed toxic protein sequence, and determining a toxic protein library on the basis of function expression characteristic data and fluctuation trend characteristic data of the consensus peptide structure; and constructing a target toxic protein core peptide set capable of covering the key functional domain of the confirmed toxic protein sequence. And performing function screening on the to-be-identified protein sequence on the functional feature domain by using the target toxic protein core peptide set to obtain an intermediate protein sequence which needs to be verified whether to conform to the toxic feature, performing toxicity screening on the intermediate protein sequence on the toxic feature domain, and determining the intermediate protein sequence conforming to the toxic feature as a target toxic protein sequence. And finally, constructing a toxic protein database by using the target toxic protein sequence. Therefore, through a two-layer screening mode combining function screening and toxicity screening, the toxic protein library building method which can discover new toxic proteins and detect low-abundance toxic proteins and is wide in coverage range is achieved.

The invention provides fusion protein containing snake venom protein and application of the fusion protein in biosynthesis of stable isotope labeled snake venom protein, and belongs to the technical field of genetic engineering and protein preparation. The fusion protein comprises a 6-poly-histidine tag, a fusion tag, a restriction enzyme cutting site of WELQ protease and snake venom protein which are connected in sequence. According to the fusion tag, the solubility of the snake venom protein fusion protein can be improved; the 6-poly-histidine tag and the fusion tag can be removed through enzyme digestion of WELQ protease, the enzyme digestion removal effect is good, and the natural structure and activity of the snake venom protein can be guaranteed; besides, a culture medium containing a unique nitrogen source or a unique carbon source labeled by an isotope is used for carrying out induction culture on recombinant bacteria for expressing the fusion protein, the fusion protein containing the target snake venom protein is subjected to induction expression, purification, WELQ protease digestion and the like are carried out, and the stable isotope labeled snake venom protein with the purity reaching 95% or above and the labeling efficiency exceeding 91% can be obtained.

This application relates to methods for preparing viral proteins, such as influenza hemagglutinin proteins and / or influenza neuraminidase proteins, immunogenic compositions comprising the viral proteins thus obtained and vaccines comprising the same, as well as methods of using the same, in particular in the prevention and / or treatment of diseases or conditions caused by viruses, such as influenza viruses.

This application relates to methods for preparing viral proteins, such as influenza hemagglutinin proteins and / or influenza neuraminidase proteins, immunogenic compositions comprising the viral proteins thus obtained and vaccines comprising the same, as well as methods of using the same, in particular in the prevention and / or treatment of diseases or conditions caused by viruses, such as influenza viruses.

Disclosed are virus-inspired compositions and preparation methods thereof, where the compositions comprise mutant papillomavirus L1 proteins that spontaneously form capsid backbones and that are conjugated to a peptide comprising an epitope to form immune redirector capsids (IRCs). The epitopes on the peptides are designed to be recognized by a subject's immune system based on the subject's preexisting immune memory developed from the subject's past exposure to the epitope through infection or vaccination. The mutant papillomavirus L1 proteins possess three mutations including an amino-terminal truncation, a carboxy-terminal truncation, and a truncation at helix four. These mutations in the L1 protein yield capsomeres that are form non-canonical T=1 geometry capsid backbones. Disclosed are uses and methods of using the compositions in treating and / or preventing cancers in subjects in need thereof.

Techniques are provided for determining pan-HLA binding of viral proteins. A trained classifier model is operable to determine, independently per HLA, at least one of (a) an average binding prediction of overlapping peptides at each position of a viral protein, (b) a maximum value of a binding prediction of overlapping peptides at each position of the viral protein, (c) standard deviation of a binding prediction of overlapping peptides at each position of the viral protein, and (d) a combination of one or more of (a)-(c). A classification engine uses the classifier model to determine average binding predictions of overlapping peptides at each position of the viral protein independently for test HLA-I and HLA-II functional groupings, where a peptide is classified as a binder when an average binding prediction corresponding to the peptide satisfies a binding value threshold.

The invention relates to a recombinant chikungunya virus vaccine as well as a preparation method and application thereof, in particular to a recombinant protein which comprises chikungunya virus protein or a variant thereof. The chikungunya fever virus serving as a main target of a neutralizing antibody is optimized, new nanoparticle protein is formed and used for preparing the vaccine, and the vaccine can stimulate an organism to generate the neutralizing antibody aiming at the chikungunya fever virus and prevent in-vivo infection and has a good neutralizing effect.

The present invention relates to an oligonucleotide comprising: a) a nucleotide sequence of a broom-kojin-ricin loop (SRL) of a eukaryotic / mammalian 60S ribosomal subunit wherein the SRL nucleotide sequence comprises at least one adenine; and b) at least one cut-dependent marker; wherein the oligonucleotide is a single strand. Preferably, the oligonucleotides form at least one loop structure or stem-loop structure. In another aspect, the invention relates to a method of detecting active shiga toxin in a sample, the method comprising the steps of: a) providing at least one oligonucleotide according to the invention; b) providing a sample of shiga toxin to be detected; c) incubating the sample with at least one single-stranded oligonucleotide; and d) detecting a labeled signal, wherein the signal indicates the presence of Shiga toxin in the sample. The invention additionally comprises a kit comprising at least one oligonucleotide according to the invention.

The invention provides a bee venom allergy possibility auxiliary analysis method and system based on detection data, belongs to the field of bee venom allergy auxiliary diagnosis, and is used for solving the problem that the bee venom allergy possibility is difficult to intelligently and accurately analyze and predict in the related technology. Determining the allergy possibility range of the bee venom of the target patient by combining the basic information of the target patient, the detection data of the bee venom protein and a possibility range evaluation model pre-constructed based on an expert experience knowledge base; basic information of the target patient, detection data of bee venom protein and historical patient data are combined to analyze and determine the bee venom allergy possibility of the target patient in an allergy possibility range, so that the analyzed bee venom allergy possibility conforms to expert experience and historical patient data; and specific conditions of the target patient can be met in a personalized manner, so that the analyzed bee venom allergy possibility is more accurate, and better support and reference are provided for diagnosis work of doctors.

Embodiments relate to methods and compositions for preventing or treating bacterial infections, particularly Staphylococcal infections. Embodiments relate to improved non-toxigenic protein A (SpA) variants.

The application is based on machine learning model, and the polypeptide WFKDWY (166953) with potential is screened through polypeptide virtual screening technology based on the analysis of PEDV Nsp5 protein crystal structure. The sequence of 166953 is artificially synthesized by solid phase, and the polypeptide is screened by using artificially expressed PEDV Nsp5 protein through ELISA experiment. The affinity constant of the polypeptide and the target protein is identified by surface plasmon resonance experiment (SPR). The cytotoxicity of the polypeptide 166953 is tested by CCK-8 kit. The virus infection inhibiting activity of the polypeptide is tested by fluorescence quantitative PCR and indirect immunofluorescence experiment. The results show that the sequence 166953 can significantly inhibit the infection of PEDV.

The present application relates to a kind of HhUp140 of anthomyiidae trichogramma venom protein and its application, it relates to the field of protein engineering, its amino acid sequence is as shown in SEQ ID NO:1, the protein can be used to paralyze pest larvae, extend pest pupation period.The present application extends the pupation period of anthomyiidae trichogramma venom protein HhUp140, reduces the disease of crop by pest to some extent.

The present invention discloses a canine distemper virus H protein monoclonal antibody 6A4A6 and its application, which belongs to the field of monoclonal antibody technology. The present invention screened out 9 monoclonal antibodies through indirect ELISA and indirect immunofluorescence identification, and named them 2C1A1, 2D1B1, 6A4A5, 6A4A6, 6G4H1, 6G4H3, 6H4F4, 6H4H5 and 6H4H6 respectively. After indirect immunofluorescence identification, the 9 monoclonal antibodies obtained were all able to react with the vaccine strain (CDV-Onderstepoort); the neutralizing antibody test results showed that among the 9 monoclonal antibodies obtained, 6A4A5 and 6A4A6 did not react with giant panda / SX / 2014 (a highly toxic isolate), but only reacted with the vaccine strain, while 6A4A6 had a higher neutralizing titer than 6A4A5 (up to 2 6 ), which can be used to prepare detection reagents and therapeutic preparations for identifying canine distemper virus vaccine strains.

The application belongs to the field of biological medicine, and particularly relates to a kind of Bungarus multicinctus venom protein and a preparation method and application thereof.The application provides a new sperm motility promoter, which is derived from Bungarus multicinctus venom and has the application potential to be developed into a new sperm motility promoter.

The invention discloses a virus protein and adjuvant mixing and stirring device which comprises a base, a telescopic rod is arranged on the side face of the base, a connecting block is rotatably connected to the upper portion of the telescopic rod, a stirring mechanism used for stirring the interior of a stirring barrel is arranged below the connecting block, and the stirring mechanism comprises a stirring cover plate below the connecting block; an on-cover motor is fixedly connected to the upper portion of the stirring cover plate, a ratchet disc is fixedly connected to the output end of the on-cover motor, an under-disc motor is arranged below the ratchet disc, and a connecting disc is fixedly connected to the output end of the under-disc motor. The device has highly-automatic cleaning and stirring processes, and the production efficiency and safety are greatly improved. After stirring is completed, an operator only needs to control the motor push column to contract through the control box, and the device can automatically start a cleaning program. The inner wall of the stirring barrel is efficiently cleaned, so that an operator is prevented from being in direct contact with residual virus protein and adjuvant with biological activity and potential danger, and the health risk is effectively reduced.

This invention utilizes a machine learning model to analyze the S1-CTD region of the PEDV S protein crystal structure. Through virtual peptide screening, a peptide sequence of FWKEAK (110616) with potential affinity for the target protein was identified. The 110616 sequence was artificially synthesized in a solid phase, and the peptide was screened using ELISA with artificially expressed PEDV S1 protein. The affinity constant between the peptide and the target protein was determined using surface plasmon resonance (SPR) assays. The cytotoxicity of peptide 110616 was tested using a CCK-8 assay kit. Its inhibitory activity against viral infection was assessed using quantitative real-time PCR and indirect immunofluorescence assays. The results showed that the 110616 sequence significantly inhibited PEDV infection.

The invention discloses an electrochemical sensor based on TdT enzyme and CbAgo synergistic mediation and application of the electrochemical sensor in cobra venom protein detection, and belongs to the technical field of electrochemical biosensors. Through magnetic bead enrichment, CbAgo protein cleavage and TdT extension reaction, and construction of a TdT enzyme and CbAgo protein synergistically mediated protein-nucleic acid conversion technology, a detection target is converted from cobra venom protein into a nucleic acid product, then the nucleic acid product is utilized to activate a Cas12a system to cut a probe P combined with a gold electrode, finally, the probe P is converted into an electrochemical signal, and the electrochemical signal is detected. The efficient and accurate detection of the cobra venom protein is realized. The electrochemical biosensor disclosed by the invention is high in sensitivity and good in specificity, and has extremely high application value in the aspects of accurate identification and quantitative analysis of cobra venom protein.

The present application relates to the technical field of veterinary biological products. Particularly provided are a porcine epidemic diarrhea virus S protein and a related product. The amino acid sequence of a porcine epidemic diarrhea virus S protein fragment is as shown in SEQ ID NO. 4. A trimer tag is fused at the C terminus of a protective antigen in a form of the truncated porcine epidemic diarrhea virus S protein, so as to construct a trimer S protein; and after expression and purification, animal evaluation is performed to determine the immunogenicity. Upon verification, a vaccine prepared from the trimer S protein has the advantages of high safety, good immunogenicity, batch-to-batch consistency, capability of inducing piglets to generate good immune responses, etc., and therefore can effectively treat or prevent porcine epidemic diarrhea.

The invention discloses a novel toxic protein and application thereof in mosquito control, and belongs to the technical field of biological control. The invention provides a novel toxic protein Cry50Ba2-like, the amino acid sequence of the novel toxic protein Cry50Ba2-like is as shown in SEQ ID NO.2, and the nucleotide sequence of a gene Cry50Ba2-like encoding the protein is as shown in SEQ ID NO.1. The invention further provides a preparation method of the novel toxic protein Cry50Ba2-like. According to the present invention, the protein Cry50Ba2-like is expressed and purified through the recombinant engineering strain, and the insecticidal activity determination experiment results prove that the protein has efficient insecticidal activity on culex quinquefasciata larvae, can be adopted as the environmentally-friendly biological insecticide, and provides the efficient and safe novel solution for mosquito-borne disease prevention and control.

The invention discloses an application of a sebastites verrucosa venom protein neoVTX. The invention finds that the sebastes verrucosa venom protein neoVTX has the functions of inhibiting a voltage-gated sodium ion channel and mediating muscle paralysis, can inhibit activities of zebra fish and grass goldfish, and can be used as a macromolecular probe template for exploring the voltage-gated sodium ion channel and explaining clinical symptoms of stab wounds of sebastes verrucosa; the compound can be further used for developing a medicine for paralyzing muscles and a medicine for relieving stabbing poisoning of sebastes verrucosa.

The invention discloses a method for extracting venom protein of miniature parasitic wasps, and relates to the technical field of biology. The extraction method comprises the following steps: carrying out anesthesia treatment on the glassogyne brilliant, putting the treated glassogyne brilliant in a buffer solution containing a protease inhibitor, and separating out venom organs; after the venom organ is subjected to low-temperature grinding and ultrasonication treatment, disulfide bonds in protein are reduced, alkylation modification is carried out, then the protein is aggregated through a solvent to form precipitates, and the miniature parasitic wasp venom protein is obtained through separation. The systematic venom protein collection and extraction process is established for the first time, and the operation bottleneck of micro insect venom research is broken through. The extraction efficiency and integrity of venom protein are remarkably improved through key technologies such as material taking process optimization, buffer solution protection, low-temperature grinding and ultrasonication.

The present invention discloses a biological preparation for preventing and controlling mosquito larvae, wherein the active ingredients are composed of six toxic proteins, namely Cry54Ba, Cry50Ba1, Tpp80Aa1, Mpp82Aa1, Xpp88Aa1 and Cyt10Aa, and the synergistic effect is most significant when the molar ratio of the six toxic proteins is 1:1. After mixing in equal molar ratios, a higher mosquito killing activity is produced through the synergistic action of the toxic proteins, and the mosquito killing rate reaches 100%. Therefore, the combination preparation of multiple toxic proteins produces a highly effective killing activity on the harassing mosquito larvae, effectively reduces the reproduction of mosquitoes, and has a significant effect on the prevention and control of mosquitoes, and the mosquito killing toxic protein preparation of microbial origin has the characteristics of high efficiency and specificity, green environmental protection, and safety and friendliness to non-target organisms.

Techniques are provided for determining pan-HLA binding of viral proteins. A trained classifier model is operable to determine, independently per HLA, at least one of (a) an average binding prediction of overlapping peptides at each position of a viral protein, (b) a maximum value of a binding prediction of overlapping peptides at each position of the viral protein, (c) standard deviation of a binding prediction of overlapping peptides at each position of the viral protein, and (d) a combination of one or more of (a)-(c). A classification engine uses the classifier model to determine average binding predictions of overlapping peptides at each position of the viral protein independently for test HLA-I and HLA-II functional groupings, where a peptide is classified as a binder when an average binding prediction corresponding to the peptide satisfies a binding value threshold.

The invention relates to the technical field of functional protein screening, in particular to an effector with a venom protein function and application thereof. The invention provides an effector with a venom protein function. The effector comprises an A0A2W1B606 protein, an A0A2W1BGQ7 protein, an A0A2W1BE44 protein, an A0A2W1B9V0 protein, an A0A2W1BFA8 protein or an I3QC11 protein. The invention further provides a preparation method of the effector. The effector obtained by screening has high-level metabolic activity, signal transduction function and venom protein function; the method plays a key role in the interaction process of phytophagous insects-host plants, such as participation in the process of cotton bollworm invading plants, regulation of the resistance of the plants to cotton bollworm larvae, targeted inhibition of phenylpropane metabolic pathways, induction of cotton bollworm preference colonization and the like.

The application discloses an electrochemical sensor based on TdT enzyme and CbAgo synergistically mediated and application thereof in detection of cobra venom protein, and belongs to the technical field of electrochemical biosensors.The application constructs a protein-nucleic acid conversion technology mediated by TdT enzyme and CbAgo protein through magnetic bead enrichment, CbAgo protein cleavage and TdT extension reaction, converts the detection target from cobra venom protein into a nucleic acid product, and then utilizes the nucleic acid product to activate a Cas12a system to cleave a probe P combined on a gold electrode, and finally converts into an electrochemical signal, so that efficient and accurate detection of the cobra venom protein is realized.The electrochemical biosensor has high sensitivity and good specificity, and has extremely high application value in accurate identification and quantitative analysis of the cobra venom protein.

The present invention discloses a method for regulating the synthesis of ectoine in Escherichia coli, which relates to the technical field of biomedicine. The method for regulating the synthesis of ectoine in Escherichia coli disclosed by the present invention constructs a tisB toxin protein expression vector pET28a(+)-p2-22-tisB with a gene fragment of the tisB toxin protein, and transfers it into an Escherichia coli engineering bacterium capable of synthesizing ectoine, so as to obtain an Escherichia coli engineering bacterium E. coli Ect pET28a(+)-p2-22-tisB that spontaneously inhibits the synthesis of ectoine, and at the same time can ensure normal synthesis of ectoine in the early stage. The nucleotide sequence of the gene fragment of the tisB toxin protein is as shown in SEQ ID NO.1. In the first three rounds of fermentation, the yield of ectoine is similar to that of the control engineering bacterium; after the seventh round, the yield of ectoine drops sharply to 2.575 g / L, and in the twentieth round of fermentation, the yield of ectoine is zero.

The present application relates to a kind of HhMAPK15 of anthocarposa larva parasitoid venom protein and its application, it relates to the field of protein engineering, its amino acid sequence is as shown in SEQ ID NO:1, the protein can be used to inhibit pest larva to continue development.The present application will be used to inhibit pest larva to continue development on anthocarposa larva parasitoid venom protein HhMAPK15, can reduce the disease of crop by pest to a certain extent.

The application is based on machine learning model, and the polypeptide YYRKWP (168510) with potential is screened through polypeptide virtual screening technology based on the analysis of PEDV Nsp5 protein crystal structure. The sequence of 168510 is artificially synthesized by solid phase, and the polypeptide is screened by using artificially expressed PEDV Nsp5 protein through ELISA experiment. The affinity constant of the polypeptide and the target protein is identified by surface plasmon resonance experiment (SPR). The cytotoxicity of the polypeptide 168510 is tested by CCK-8 kit. The virus infection inhibiting activity of the polypeptide is tested by fluorescence quantitative PCR and indirect immunofluorescence experiment. The results show that the sequence 168510 can significantly inhibit the infection of PEDV.