Polypeptide extracted from stinging cell venom of fire medusa and application thereof

A fire jellyfish thorn and venom technology is applied in the field of peptides to achieve the effects of few separation steps, rapid separation and mild separation conditions

Inactive Publication Date: 2015-09-23
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, surgical resection and chemical drug treatment are also indispensable means for highly differentiated cancers with a late course and recurrence after radiotherapy.

Method used

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  • Polypeptide extracted from stinging cell venom of fire medusa and application thereof
  • Polypeptide extracted from stinging cell venom of fire medusa and application thereof
  • Polypeptide extracted from stinging cell venom of fire medusa and application thereof

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Experimental program
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Effect test

Embodiment 1

[0042] A polypeptide molecule, the amino acid sequence of which is shown in SEQ ID No.1;

[0043] SEQ ID No. 1:

[0044]

[0045]

Embodiment 2

[0047] The extraction of the polypeptide of the present invention:

[0048] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 0°C in a volume ratio of 1:1. After stirring and standing still, take the precipitate and repeat it twice. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0049] Step 2. Mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 5.5 according to the mass volume ratio of 1g: 40mL, and ultrasonically crush it for the second time. Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0050] Step 3, take the venom protein obtained in step 2, dilute it 3 times with a buffer solution of disodium hydrogen phosphate-sodium dihydrogen ...

Embodiment 3

[0054] The extraction of the polypeptide of the present invention:

[0055] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 4°C at a volume ratio of 1:1.5. After stirring and standing still, take the precipitate and repeat it for 4 times. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0056] Step 2, mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 6.5 according to the mass volume ratio of 1g: 60mL, and ultrasonically crush it for the second time Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0057] Step 3, take the venom protein obtained in step 2, dilute it 5 times with the disodium hydrogen phosphate-sodium dihydrogen phosphate ...

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Abstract

The invention discloses a polypeptide extracted from stinging cell venom of a fire medusa. The amino acid sequence of the polypeptide is showed in the SEQ ID No.1. The invention further discloses the extraction method for the polypeptide. The extraction method comprises the steps that venom protein is extracted from the stinging cell venom of the fire medusa, then a Histrap SP strong positive ion exchange column and a Histrap Q strong anion exchange column are used sequentially to separate the polypeptide in the venom protein. The invention further discloses the application of the polypeptide in preparation of anti-nasopharynx-cancer medicine. The polypeptide can restrain the growth of a nasopharynx cancer cell strain CNE-1 and the growth of a nasopharynx cancer cell strain CNE-2 and induce the nasopharynx cancer cell to die. The extraction method for the polypeptide extracted from the stinging cell venom of the fire medusa has the advantages that the operation is simple, the separation procedures are few, the extraction method is suitable for quick separation, the separation condition is mild, and the anti-tumor activity of the obtained protein can be reserved to the largest content.

Description

technical field [0001] The invention relates to the fields of marine biological technology and medicine, in particular to a polypeptide extracted from the nematode venom of Pyrogus jellyfish and its application. Background technique [0002] Tamoya alata Uchida is a large and highly toxic jellyfish that is only distributed in the Beibu Gulf of my country. It has long tentacles and a large number of nematocyst cells. It contains venom whose main active ingredient is protein / polypeptide. When people or animals come into contact, the nematocysts will penetrate into the skin, penetrate deep into the dermis, and release the toxins stored in the cells, causing stings. Depending on the severity of the injury, the victim will experience skin redness, swelling, tingling, bleeding, and skin ulceration immediately at the stung site. In severe cases, the victim will become weak, have fever, vomit, and even die. The reason is that the stinging cells of jellyfish tentacles contain a large...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/18A61K38/17A61P35/00
CPCA61K38/00C07K14/43595
Inventor 屈啸声夏祥华钟桃珍樊兰兰李刚
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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