69results about How to "Mild separation conditions" patented technology

A process for preparing the non-sugar tea beverage by use of high-voltage pulse electric field to sterilize it includes such steps as extracting from tealeaves in hot water, filter, quick cooling. Centrifugal separation of deposit, filtering by Millipore membrane and sterilizing by high-voltage pulse electric field. Its advantages are no loss of tea polyphenol and taste substances, and long storage time.

The invention discloses a method for extracting silk peptide from cocoon fiber, which belongs to the technical field of preparation of the silk peptide. The method comprises the following steps: the cocoon fiber is added into a Na2CO3 solution for refining, filtrated and added into a CaCl2 solution for dissolution; protease for silk dissolution solution obtained after dissolution undergoes enzymatic hydrolysis reaction; after ultrafiltraiotn and nano-filtration separation of digest, a feed liquid is obtained; and the silk peptide powder is obtained after spray drying of the feed liquid. The method has the advantages that ACE inhibitory polypeptide is enriched through ultrafiltraiotn and nano-filtration separation of the digest; the efficiency of removing CaCl2 salt is high; the method has the advantages of large processing capacity, mild separation condition, simple flow, low energy consumption and so on; and the industrial production is easy to realize.

The invention relates to a method for preparing selenium-rich active peptide loach nutrient solution or powder, pertaining to bioengineering and membrane technology field. Enzymatic hydrolysis-nanofiltration concentration technique is adopted in the preparation method of the selenium-rich active peptide loach nutrient solution or powder in the method for preparing the selenium-rich active peptide loach nutrient solution or the powder; loach is utilized as material and water in a proportion of 1: 1 is added; enzymolysis reaction is caused in case of pH 3-10 of protease and at a temperature of 30 to 60 DEG C, the dosage of protease is 1 to 3 percent of the total weight of uniform loach slurry and water and the enzymolysis reaction lasts for 2 to 10 hours; the degree of hydrolysis is controlled between 12 to 20 percent through a pH-STAT or TNBS method; after the completion of the reaction, the protease is inactivated and the obtained solution is centrifugally filtered to obtain the enzymolysis solution which is concentrated and desalted with a nanofiltration membrane so that the selenium-rich active peptide loach nutrient solution is prepared, which can be sprayed and dried to obtain the selenium-rich active peptide loach nutrient powder. The method for preparing the selenium-rich active peptide loach nutrient solution or the powder has the advantages of large processing quantity, moderate separation conditions, simple process and low energy consumption, and can be scale manufactured.

The invention provides a preparation method for a thiophilic composite microsphere and a novel method for separating and purifying serum immunoglobulin G (IgG) with high purity and high activity in batch, which has simple and convenient operation. In the invention, ferroferric oxide particles with superparamagnetism are prepared by redox reaction, magnetic microspheres are prepared by miniemulsion polymerization technology, the full and complete coating of magnetic particles with divinylbenzene monomers is realized making use of the concentration difference of moisture repel and vinyl ester is added to carry out a second coating, and the magnetic microspheres are prepared by free radical polymerization. After full alcoholysis reaction, large quantity of active functional groups appear on the surface of the magnetic microspheres, which the thiophilic magnetic composite microsphere is formed by Michael addition reaction and grafting thiophilic petunidin such as sulfhydryl-nicotinic acid, etc., thus realizing specific recognition and separation for human immunoglobulin.

The invention discloses a nano particle separation and detection method. The nano particle separation and detection method comprises the following steps: a, pretreating an aqueous solution containing nano particles; b, separating a product obtained from the step a by adopting an asymmetric flow field flow separation technology; and c, determining the concentration of nano particles within different particle diameter ranges, obtained in the step b by separating. According to the invention, the asymmetric flow field flow separation technology is adopted, and is a novel nano particle separation technique. Compared with the traditional separation technique, the asymmetric flow field flow separation technology is mild in separation condition, and a solid phase does not exist in a separation flow channel; the asymmetric flow field flow separation technology is rapid in separation, is high in resolution, and is wide in separation range (1nm-10 microns); the flow phase can be selected from any solvent, and special pretreatment of a sample is not required; a sample is concentrated automatically; detectors, such as UV, MALS and ICP-MS can be connected online.

The invention provides an olefin/alkane separation membrane and a preparation method thereof. The olefin/alkane separation membrane comprises a supporting membrane layer and a separation membrane layer which are sequentially stacked, wherein the separation membrane layer is composed of a polyether-polyamide block copolymer and ionic liquid with a transition metal ion complex as a cation. Accordingto the invention, the synergistic effect of the polyether-polyamide block copolymer and the components in the ionic liquid taking the transition metal ion complex as the cation is utilized; the permeability coefficient of the olefin/alkane separation membrane can be increased to 33*10<-10>/cm<3>(STP).cm/(cm<2>.S.cmHg) or above, the separation coefficient of olefin can reach 7 or above, the service life of the membrane can reach 30 days or above under the condition that moisture wetting is not needed, and the olefin/alkane separation membrane is suitable for further application and popularization.

The invention relates to a protein separation and purification method and particularly relates to a method for extracting protein from sesame. The method comprises the following steps of: performing ultrafine crushing on the sesame to form powder, preparing a sesame protein leaching solution, performing cloud point phase separation extraction, and performing dialysis purification to prepare the sesame protein. According to the method disclosed by the invention, a cloud point extraction method is utilized for extracting the protein, thus the using quantity of an organic solvent is low, the operation is simple, and the large-scale operation is facilitated; the nature of the sesame protein is fully utilized, the water solubility under basic conditions is strong, cloud point extraction is performed twice, and the sesame protein with few impurities and high purity is obtained; and the method further has the advantages of low using quantity of surfactant, low cost, simple system composition, high extraction rate, short preparation cycle and mild separation conditions.

The invention provides cytochrome C and an endotoxin removing method thereof, and relates to the technical field of purification. The cytochrome C is prepared by removing endotoxin by the endotoxin removing method: anion resin is put into a chromatographic column; buffer liquid is used for balancing the chromatographic column, so that the pH value and the electrical conductivity of effluent liquid are consistent with the pH value and the electrical conductivity of the buffer liquid of the chromatographic column; after the pH value of a fine solution of the cytochrome C with unqualified endotoxin is regulated to be consistent with the pH value of the buffer liquid, filtering is performed to obtain clear sample loading liquid; after the sample loading liquid passes the column, flow through liquid is obtained; the collected flow through liquid is sequentially subjected to dialysis and filtering. The qualified rate of the endotoxin of the cytochrome C prepared by the method is high; the preparation process is simple; the separation effect is good; the repeatability is high; the sample recovery rate is high; the separation conditions are mild; higher industrial popularization prospects are realized.

The invention discloses a method for preparing polyphenol by using cinnamon residues. The method comprises the following steps: (1) mixing cinnamon residue dry powder and ethanol, stirring, extracting, standing, filtering, extracting, combining extracting liquid, evaporating and drying; (2) mixing the product obtained in the step (1) and petroleum ether, extracting, recovering petroleum ether from supernatant liquid, adding the extractant into ethyl acetate, extracting, combining the supernatant liquid, evaporating the ethyl acetate and performing freeze drying after solid is frozen to obtain a cinnamon residue polyphenol crude extract. The cinnamon residue polyphenol extract is extracted from the cinnamon residues, and the preparation method is simple, high in extraction efficiency, low in production cost, high in safety and suitable for industrialized production.

The invention discloses an aqueous two-phase method for efficiently extracting phycoerythrin. The aqueous two-phase method comprises the following steps: (1) drawing an aqueous two-phase diagram; (2) selecting two-phase points, and carrying out a preliminary experiment; (3) comparing the extraction purity of different salt systems, and selecting an appropriate PEG (polyethylene glycol)/salt system; (4) comparing the influence of different parameters such as different molecular masses of PEG, different system line lengths, different volume ratios and different pH values on purity; and (5) carrying out multiple aqueous two-phase experiments, and selecting the optimal number of times. The aqueous two-phase method simplifies a protein purification process, is easy to operate on a large scale, low in cost, high in yield, high-efficiency and environment-friendly.

The invention discloses earthworm protein polypeptide wine and a preparation method thereof. The earthworm protein polypeptide wine is prepared from 1.5 to 2.5 parts of earthworm protein polypeptide, 8 to 14 parts of semen plantaginis, 8 to 14 parts of medulla tetrapanacis, 5 to 8 parts of rhizoma alismatis, 5 to 8 parts of junci medulla and 75 to 85 parts of yellow rice wine with the alcohol content of 10 to 15%. According to the earthworm protein polypeptide wine disclosed by the invention, the earthworm protein polypeptide is utilized as a main ingredient, and the earth worm protein polypeptide is matched with traditional Chinese adjuvant herbs of the semen plantaginis, the medulla tetrapanacis, the rhizoma alismatis and the junci medulla to be prepared into health-care medicated wine which improves urine output and has diuretic activity; when the main ingredient and the traditional Chinese adjuvant herbs are matched, a synergistic interaction effect is achieved, and diuretic activity is enhanced and can be as long as 7 hours; the earthworm protein polypeptide wine has the characteristic of slow release, is suitable for long term administration and reduces untoward effects.

The invention discloses a method for preparing polyphenols from betel dregs, which comprises the following steps: (1) mixing betel dregs dry powder with ethanol, stirring, extracting, standing, filtering, extracting, combining extracts, and evaporating to dryness; ( 2) Mix the product obtained in step (1) with petroleum ether, extract, supernatant and recover petroleum ether; add the extract to ethyl acetate, extract, combine the supernatant to evaporate ethyl acetate, freeze the solid and freeze-dry Obtain the crude extract of betel slag polyphenols. The betel lang polyphenol extract is extracted from betel langlag, the preparation method is simple, the extraction efficiency is high, the production cost is low, the safety is high, and it is suitable for industrial production.

The invention relates to a method for preparing special asphalt from coal tar through progressive separation. The method comprises the following specific steps: proportionally adding a mixed solvent Ainto the coal tar, carrying out stirring, carrying out standing, and carrying out layering, so as to obtain an upper-layer mixed solution A; taking the upper-layer mixed solution A, carrying out filtering, and carrying out distilling to recover the mixed solvent A, so as to obtain a first-stage separated product of the coal tar; proportionally adding a mixed solvent B into the first-stage separated product of the coal tar, carrying out stirring, carrying out standing, and carrying out layering, so as to obtain an upper-layer mixed solution B; taking the upper-layer mixed solution B, and carrying out distilling to recover the mixed solvent B, so as to obtain a light component of the coal tar; proportionally adding a mixed solvent C into the light component of the coal tar, carrying out intensive mixing, carrying out standing, and carrying out layering, so as to obtain an upper-layer mixed solution C; taking the upper-layer mixed solution C, carrying out filtering, and carrying out distilling to recover the mixed solvent C, thereby obtaining the special asphalt. According to the method, the special asphalt can be obtained from the coal tar through progressive separation at normal temperature under normal pressure and can be applied to high-end carbon-material raw materials, and the requirements of subsequent preparation on products with high added values are met.

This invention relates to a separation system of DNA binding protein magnetism nanometer particle, composed by follows component: magnetism nanometer particle with strepto avidin surface finish; double strand DNA noted biotin; Nucleoprotein bonded with double strand DNA; and external magnetic field. The linkage of magnetic nanometer particle and long chain dsDNA is high efficiency and singleness. The protein and DNA keep each other idiosyncratic combine at optimal identify system in the separation process. The magnetic separation elution method has gentle condition, not create damage to sample, and opreation simple. You can use this nanometer separation system to obtain DNA binding protein, supply reliable technology platform for DNA and protein interaction research.

The invention discloses a separation and purification method of sugammadex. The method comprises the steps: firstly, dissolving a sugammadex sample with a mixed solution, and filtering out insoluble substances; then loading the filtered sample solution to an ODS column; separating by adopting a single-column internal circulation mode or a double-column internal circulation mode; and finally, carrying out gradient elution or isocratic elution on the sample by taking the mixed solvent as a mobile phase, collecting target peaks in sections, and summarizing components of which the target peaks meet the requirements to obtain a purified sample. The method provided by the invention has the advantages of mild separation conditions, high separation efficiency, high separation speed, high universality and the like, can well realize sample separation and purification, can realize enrichment in a short time, can purify and even other types of compounds by changing elution conditions and switchingvalves, and has a good application prospect.

The present invention discloses a separation method for acetophenone and 1-phenylethanol. According to the difference of the solubility and the relative volatility of acetophenone and 1-phenylethanol in glycerol, the separation of the acetophenone and 1-phenylethanol is realized by utilizing the coupling operation of extraction and distillation. The separation method has the advantages of simple process, good separation effect, energy conservation, environment protection and the like, and is suitable for industrial large-scale continuous operation.