69results about How to "Mild separation conditions" patented technology

The invention discloses a separation method of acetophenone from 1-phenethyl alcohol. The solubility and the relative volatility of acetophenone and 1-phenethyl alcohol in glycerine are different, so that separation of acetophenone from 1-phenethyl alcohol is realized via coupling operation of extraction and rectification. The separation method is simple; separation effect is excellent; energy is saved; the separation method is friendly to the environment, and is suitable for industrial-scale continuous operation.

The invention discloses a separation and extraction method of garlic oil. The separation and extraction method comprises the following steps: peeling and crushing garlic into garlic puree, adding water for enzymolysis, then heating and preserving heat, further performing steam distillation, performing heat exchange and cooling with steam, and then condensing to get emulsion of the garlic oil and water; filtering the emulsion by using a micro-filtration membrane; placing the emulsion on the penetration side, which is obtained by micro-filtration, in a penetration and steaming device for separation; and condensing the steam which is collected at the penetration side of the penetration and steaming device to obtain the garlic oil. According to the separation and extraction method, penetration and steaming are adopted for further separation of the garlic oil on the basis of steam distillation, the separation conditions are mild, and the separation tasks which are difficult to complete by distillation, extraction, absorption and other traditional methods can be realized with low energy consumption. The separation and extraction method is particularly suitable for mixtures with near boiling points and constant boiling point, which are more difficult to separate or can not be separated by a distillation method, the problem that oil-water separation is not complete in the steam distillation can be also well solved, and the separation and extraction method is considered to be an alternative method with great prospects.

The invention provides a method for preparing oligomeric proanthocyanidins by utilizing a grape seed extract, belonging to the technical field of separation and preparation of the oligomeric proanthocyanidins. According to the invention, oligomeric proanthocyanidins freeze-dried powder and polymeric proanthocyanidins freeze-dried powder are prepared through dissolving, filtering, vacuum concentration, ultrafiltration, column filling, elution, nanofiltration and freeze-drying by using the grape seed extract as a raw material. The method provided by the invention has high raw material utilization rate, high separation accuracy, good separation effect, high product purity and clear product use, realizes complete utilization of raw materials and complete recycling of the solvent, is free of three-wastes discharge, and is a typical high-tech green process. The oligomeric proanthocyanidins prepared by using the method provided by the invention can be extensively applied in the fields of medicines, health-care products, etc.

The invention discloses a method for extracting soybean protein and purifying and recovering a surfactant, which mainly includes the following steps: (1) preparing a mixed reverse micelle system; (2) mixing soybean powder with the reverse micelle system, and ultrasonically assisted extraction , to obtain a pre-extract that contains protein and oil; (3) the protein pre-extract is rotatably evaporated to reclaim the organic solvent; (4) the buffer solution and the protein pre-extract are mixed and back-extracted to obtain the protein aqueous solution and mixed oil; (5) respectively The aqueous protein solution and the mixed oil are purified by alcohol washing, and the centrifuged mixed liquid is separated by column chromatography to recover the surfactant. The advantages of the present invention: the use of the composite reverse micelles system can reduce the impact of surfactants on protein activity; mixed surfactants are beneficial to the extraction of high molecular weight proteins; a certain amount of solvent is removed by rotary evaporation before protein extraction after reverse micelles, The consumption of buffer solution, the amount of waste water discharge and the energy consumption required for drying protein in the process of extracting protein after reverse micelles are reduced.

The invention discloses a nano particle separation and detection method. The nano particle separation and detection method comprises the following steps: a, pretreating an aqueous solution containing nano particles; b, separating a product obtained from the step a by adopting an asymmetric flow field flow separation technology; and c, determining the concentration of nano particles within different particle diameter ranges, obtained in the step b by separating. According to the invention, the asymmetric flow field flow separation technology is adopted, and is a novel nano particle separation technique. Compared with the traditional separation technique, the asymmetric flow field flow separation technology is mild in separation condition, and a solid phase does not exist in a separation flow channel; the asymmetric flow field flow separation technology is rapid in separation, is high in resolution, and is wide in separation range (1nm-10 microns); the flow phase can be selected from any solvent, and special pretreatment of a sample is not required; a sample is concentrated automatically; detectors, such as UV, MALS and ICP-MS can be connected online.

The invention discloses a method for preparing biologically active peptide-silk fibroin peptide, which belongs to the field of food biotechnology. In the present invention, silkworm cocoons or waste silk are used as raw materials, and 0.3-1.0% (w / v) Na 2 CO 3 After the solution is refined, use 20-60% (w / w) CaCl 2 The solution is heated and dissolved to obtain the silk-dissolving liquid, and the silk-dissolving liquid is hydrolyzed with protease at pH 2.5-12.0 and 30-80°C, and then the high-concentration CaCl in the enzymatic hydrolysis liquid is removed by nanofiltration technology 2 Salt and concentrated solution are spray-dried to obtain silk fibroin peptide powder. The invention has the advantages of large processing capacity, high desalination rate, mild separation conditions, simple process and low energy consumption, can realize industrial production, and prepare high-purity silk fibroin peptide powder for the development of functional food.