Kit for detecting susceptibility gene SNP locus of nasopharynx cancer

Abstract

The invention relates to a kit for detecting susceptibility gene SNP locus of nasopharynx cancer, which is realized through the steps as follows: detecting a PCR amplimer and a single-base extension primer of an SNP locus rs2860580 of a susceptibility gene CDKN2A-CDKN2B of nasopharynx cancer; detecting PCR amplimers and single-base extension primers of SNP loci rs1572072 and rs9510787 of a susceptibility gene TNFRSF19 area of nasopharynx cancer; detecting PCR amplimers and single-base extension primers of SNP loci rs28421666,rs2860580 and rs2894207 of a susceptibility gene HLA of nasopharynx cancer; and detecting a PCR amplimer and a single-base extension primer of an SNP locus rs6774494 of a susceptibility gene MDS1-EVI1 of nasopharynx cancer. The kit provided by the invention can be used for simultaneously detecting the SNP loci of a plurality of susceptibility genes of nasopharynx cancer, and provides reference for estimating risk degree of individuals affected by nasopharynx cancer, general survey of populations with high nasopharynx cancer incidence, screening of high risk group attacked by nasopharynx cancer and implementation of relative precautionary measures.

Description

technical field [0001] The invention relates to a kit for detecting SNP sites of nasopharyngeal carcinoma susceptibility genes. Background technique [0002] Nasopharyngeal carcinoma is a malignant tumor derived from the epithelium of the nasopharynx. It mainly occurs in the top of the nasopharynx and pharyngeal recesses. It is a complex disease affected by multiple factors. Its occurrence and development are closely related to genetic factors, environmental factors and EBV virus infection. China is a high-incidence area of ​​nasopharyngeal carcinoma in the world. According to the statistics of the World Health Organization, in 2008, new cases in China accounted for 39.2% (33,101 people) of the world, and deaths due to nasopharyngeal cancer accounted for 40.5% (20,899 people) of the world; and, nasopharyngeal cancer Cancer is prone to occur in the prime of life, seriously affecting people's health and economic development. [0003] In recent years, the modern "3P" medical c...

AI Technical Summary

Problems solved by technology

However, there is currently a lack of effective NPC risk prediction products

Although individual patent applications have been made for the method of determining whether an individual is susceptible to nasopharyngeal carcinoma based on the C-2128T locus, C-1888T locus of the nasopharyngeal carcinoma-related gene PLUNC, or the haplotype composed of these two loci (details See patent: CN200510008650.1 "A method for detecting the PLUNC genotype of the susceptibility gene related to nasopharyngeal carcinoma and the PLUNC susceptibility gene"), however, this method only detects a very small number of indicators, and the accuracy is low; and , the reliability of this method needs to be verified by large-scale population samples

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