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Kit for detecting susceptibility gene SNP locus of nasopharynx cancer

A susceptibility gene, nasopharyngeal cancer technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems that need to be verified by large-scale population samples, lack of nasopharyngeal cancer incidence risk prediction products, accuracy rate lower problem

Inactive Publication Date: 2013-04-17
SUN YAT SEN UNIV CANCER CENT
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Problems solved by technology

However, there is currently a lack of effective NPC risk prediction products
Although individual patent applications have been made for the method of determining whether an individual is susceptible to nasopharyngeal carcinoma based on the C-2128T locus, C-1888T locus of the nasopharyngeal carcinoma-related gene PLUNC, or the haplotype composed of these two loci (details See patent: CN200510008650.1 "A method for detecting the PLUNC genotype of the susceptibility gene related to nasopharyngeal carcinoma and the PLUNC susceptibility gene"), however, this method only detects a very small number of indicators, and the accuracy is low; and , the reliability of this method needs to be verified by large-scale population samples

Method used

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  • Kit for detecting susceptibility gene SNP locus of nasopharynx cancer
  • Kit for detecting susceptibility gene SNP locus of nasopharynx cancer
  • Kit for detecting susceptibility gene SNP locus of nasopharynx cancer

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Embodiment Construction

[0017] 1. SNP sites (7) in the genome of the subject detected by the kit of the present invention

[0018] Through the genome-wide association analysis of nasopharyngeal carcinoma with a large sample size, the inventors obtained the SNPs significantly related to the incidence of nasopharyngeal carcinoma. In addition, combined with HapMap, dbSNP and other databases, the SNP sites were selected, and the success rate of site experiments was scored to determine 7 SNP loci to be studied. Among them, rs1412829 is located in the gene CDKN2A-CDKN2B region, rs1572072 and rs9510787 are located in the gene TNFRSF19 region, rs2860580, rs2894207 and rs28421666 are located in the gene HLA region, and rs6774494 is located in the gene MDS1-EVI1 region.

[0019] Table 1. Seven SNPs significantly associated with the incidence of nasopharyngeal carcinoma

[0020]

[0021]

[0022] 2. Design and synthesize PCR amplification primers and single-base extension primers for the SNP sites to be ...

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Abstract

The invention relates to a kit for detecting susceptibility gene SNP locus of nasopharynx cancer, which is realized through the steps as follows: detecting a PCR amplimer and a single-base extension primer of an SNP locus rs2860580 of a susceptibility gene CDKN2A-CDKN2B of nasopharynx cancer; detecting PCR amplimers and single-base extension primers of SNP loci rs1572072 and rs9510787 of a susceptibility gene TNFRSF19 area of nasopharynx cancer; detecting PCR amplimers and single-base extension primers of SNP loci rs28421666,rs2860580 and rs2894207 of a susceptibility gene HLA of nasopharynx cancer; and detecting a PCR amplimer and a single-base extension primer of an SNP locus rs6774494 of a susceptibility gene MDS1-EVI1 of nasopharynx cancer. The kit provided by the invention can be used for simultaneously detecting the SNP loci of a plurality of susceptibility genes of nasopharynx cancer, and provides reference for estimating risk degree of individuals affected by nasopharynx cancer, general survey of populations with high nasopharynx cancer incidence, screening of high risk group attacked by nasopharynx cancer and implementation of relative precautionary measures.

Description

technical field [0001] The invention relates to a kit for detecting SNP sites of nasopharyngeal carcinoma susceptibility genes. Background technique [0002] Nasopharyngeal carcinoma is a malignant tumor derived from the epithelium of the nasopharynx. It mainly occurs in the top of the nasopharynx and pharyngeal recesses. It is a complex disease affected by multiple factors. Its occurrence and development are closely related to genetic factors, environmental factors and EBV virus infection. China is a high-incidence area of ​​nasopharyngeal carcinoma in the world. According to the statistics of the World Health Organization, in 2008, new cases in China accounted for 39.2% (33,101 people) of the world, and deaths due to nasopharyngeal cancer accounted for 40.5% (20,899 people) of the world; and, nasopharyngeal cancer Cancer is prone to occur in the prime of life, seriously affecting people's health and economic development. [0003] In recent years, the modern "3P" medical c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156
Inventor 曾益新贾卫华崔倩贝锦新阮红莲
Owner SUN YAT SEN UNIV CANCER CENT
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