Artificially synthesized insect-resistant protein mCry1Ia2 as well as preparation method and applications thereof

A technology of artificial synthesis and anti-insect protein, applied in the fields of genetic engineering and biological control, to achieve the effect of reducing usage, broad application prospects and important economic value
CN111995690AActive Publication Date: 2020-11-27黑龙江大鹏农业有限公司 +1
15 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
黑龙江大鹏农业有限公司
Publication Date
2020-11-27

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses artificially synthesized insect-resistant protein mCry1Ia2. The invention also discloses a nucleic acid molecule of the protein, and a recombinant vector, a host cell or a recombinant bacterium of the nucleic acid molecule. The invention also discloses applications of the protein, the nucleic acid molecule and the recombinant vector of the nucleic acid molecule, the host cell or the recombinant bacteria to regulation and control of plant insect resistance, insect killing or improvement in insect resistance of transgenic plants, and preparation of insecticides or insectcontrol preparations. In vitro experiments show that the modified Bt toxin-producing protein has higher insecticidal activity against ostrinia nubilalis, and has significant insecticidal effect on thepopulation of ostrinia nubilalis resistant to Cry1Ab, Cry1F and Cry1Ie toxin protein. A novel plant expression vector is constructed by a constitutive promoter, and the insect-resistant gene mCry1Ia2can be stably and efficiently expressed in monocotyledonous plants to be further used for producing the insect-resistant transgenic plants.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the fields of genetic engineering and biological control, in particular to an artificially synthesized insect-resistant protein mCry1Ia2 and a preparation method and application thereof. Background technique

[0002] The Bt gene encodes an insecticidal crystal protein from Bacillus thuringiensis, which is widely present in water, soil, desert, leaves, and insect carcasses, and is currently the most productive and widely used bioinsecticide. When it forms spores, it can form insecticidal crystal protein (ICP), also known as delta-endotoxin, which can produce specific insecticidal activity against agricultural pests such as Lepidoptera and Coleoptera. Its action process includes the ingestion of protoxin by insects, the enzymatic hydrolysis and activation of protoxin, the toxin passing through the peritrophic membrane, the binding of toxin to specific receptors, the embedding of toxin molecules into the epidermal cell membrane, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More