Recombinant protein highly targeting SARS-ConV-2 virus S protein extracellular BD-terminal domain and subunit vaccine thereof

A sars-conv-2, recombinant protein technology, applied in the direction of positive-sense single-stranded RNA viruses, viruses, viral peptides, etc., can solve problems such as breaking the immune balance of the body, achieve good development and application prospects, and improve immune stress ability. Effect
CN113151331AActive Publication Date: 2021-07-23WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN UNIV
Publication Date
2021-07-23

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Abstract

The invention provides a recombinant protein highly targeting SARS-ConV-2 virus S protein extracellular BD-terminal domain and a subunit vaccine thereof. A nucleotide sequence for coding the recombinant protein is as shown in SEQ ID NO: 5 or SEQ ID NO: 6. The novel coronavirus subunit vaccine comprises the recombinant protein and a pharmaceutically acceptable adjuvants. According to the invention, the recombinant protein highly targeting SARS-ConV-2 virus S protein extracellular BD-terminal domain is prepared by the following steps: screening S protein extracellular domain surface antigenic determinants to find Sc and Se peptide fragment gene sequences capable of stimulating cells to generate strong immune response, carrying out codon optimization, cloning into a plasmid vector containing a diphtheria toxin T structure, and carrying out secretory expression and purification. Experiments show that the purified recombinant protein can induce specific cell reaction, is safe and efficient, and has good development and application prospects.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a highly targeting recombinant protein of the outer BD end domain of SARS-ConV-2 virus S protein and a subunit vaccine thereof. Background technique

[0002] SARA-Cov-2 belongs to the genus β-coronavirus of the Coronaviridae family, and is related to severe acute respiratory syndrome (severe acute respiratory syndrome, SARS) coronavirus (SARS-CoV) and Middle East respiratory syndrome (Middle East respiratory syndrome, MERS) coronavirus ( MERS-CoV) belong to the same genus. SARA-Cov-2 is a positive-strand single-stranded RNA virus with a genome of 29.9kd. For RNA viruses, the genome is relatively large, encoding a total of 9860 amino acids. The genome includes a cap structure at the 5' end and a poly A tail at the 3' end. The replicase genes encoding 16 non-structural proteins are close to the 5' end, accounting for about 2 / 3 of the entire genome, and the remaining 1 / 3 of the genome en...

Claims

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