Compound, protein, preparation method of compound, and preparation method of protein

A protein and complex technology, which is applied in the field of protein and their preparation and complex, can solve the problems of short timeliness and inability to realize oral administration, and achieve the effect of improving purity, strong stability, safety and quality research convenience

Inactive Publication Date: 2017-08-11
天津博锐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The first object of the present invention is to provide a complex to alleviate the technical problems in the prior art that the use of glucagon-like peptide-1 to reduce blood sugar cannot be taken orally and has a short time effect

Method used

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  • Compound, protein, preparation method of compound, and preparation method of protein
  • Compound, protein, preparation method of compound, and preparation method of protein
  • Compound, protein, preparation method of compound, and preparation method of protein

Examples

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preparation example Construction

[0051] The present invention aims at the technical problems of unsatisfactory protein yield and initial purity in the prior art, and provides a protein preparation method, comprising:

[0052] Adjust the pH of the yeast fermentation broth to 2.0-4.0, let it stand for 8-12 hours, heat it to 75-85°C, perform physical deoxygenation, centrifuge and collect the precipitate.

[0053] Among them, the pH value can be, for example, but not limited to 2.0, 3.0 or 4.0; the storage time can be, for example, but not limited to 8h, 9h, 10h, 11h or 12h; the heating temperature can be, for example, but not limited to 75°C, 80°C or 85°C.

[0054] In the present invention, the protein is as follows (1), (2) or (3):

[0055] (1) QQCTTGQLQCCESTSTANDPATSELLGLIGVVISDVDALVGLTCSPISVIGVGSGSACTANVCDSSPIGGLVSIIGCVPVNV (SEQ ID NO. 1);

[0056] (2) QQCTTGQLQCCESTSTANDPATSKLLGLIGVVISDVDALVGLTCSPISVIGVGSGSACTANVCDSSPIGGLVSIIGCVPVNV (SEQ ID NO. 2);

[0057] (3) The amino acid sequence of SEQ ID NO.1 or SEQ ...

Embodiment 1p

[0084] Example 1pH value

[0085] A method for preparing protein, comprising:

Embodiment 1-1

[0087] Adjust the pH of the Pichia yeast fermentation broth to 3.0;

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Abstract

The invention provides a compound, a protein, a preparation method of the compound, and a preparation method of the protein, and relates to the technical field of biomedicines. The compound has high stability, can be orally administered on the basis of the long-acting blood sugar lowering function of glucagon-like peptide-1, lays a foundation for the further development of diabetes related drugs, and deserves subsequent continuous development. The preparation method of the protein is characterized in that physical deoxidation is carried out at 75-85 DEG C, so the purity of the protein is greatly increased; and the protein is prepared through using the preparation method of the protein, and the purity reaches 80% or above. A protein purification technology provided by the present invention can remove the heat toxin proteins and nucleic acid substances introduced by yeast fermentation, and brings convenience for the researches on the safety and the quality of the protein used as a medicinal preparation in the later application stage.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a complex, a protein and a preparation method of the two. Background technique [0002] Diabetes mellitus (DM) is a metabolic disease characterized by relative or absolute insufficiency of insulin. Relative or absolute insufficiency of insulin leads to hyperglycemia, which in turn leads to metabolic disorders of the three major nutrients, ultimately affecting the normal physiological functions of patients and causing complications. Diabetes is increasing worldwide and is expected to reach 439 million by 2030. [0003] Glucagon-like peptide-1 is a 37 amino acid peptide secreted by intestinal L-cells in response to food intake. Glucagon-like peptide-1 has been found to stimulate insulin secretion (insulinotropic activity), resulting in cellular uptake of glucose and a decrease in serum glucose levels. However, glucagon-like peptide-1 has very low activity and a serum half-lif...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12P21/00C12R1/84
CPCC07K14/39C07K14/605C07K19/00
Inventor 高井珍
Owner 天津博锐生物科技有限公司
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