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EB virus BALF4 protein epitope

An antigenic epitope, EB virus technology, applied in the field of molecular biology and immunology, can solve problems such as lack of, difficulty in determining highly conserved antigenic domains, and limitation of BALF4 expression detection

Active Publication Date: 2021-10-12
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, due to the high variability of the Epstein-Barr virus genome, although the sequence of BALF4 has been registered in the NCBI database, it is still difficult to determine the highly conserved and highly specific antigenic domain. The BALF4 antibody used in scientific research, and therefore, the detection of BALF4 expression in tumor tissue and the study of its pathogenic mechanism are greatly limited

Method used

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  • EB virus BALF4 protein epitope
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  • EB virus BALF4 protein epitope

Examples

Experimental program
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Embodiment

[0051] 1. The present invention analyzes and detects the Epstein-Barr virus sequence in the WGS data of 31 EB virus-positive NK / T-cell lymphoma patients, extracts the sequence information of BALF4, and identifies a single nucleotide variation site and amino acid variation site, Compare with the B95-8 reference sequence, identify the changed amino acids, and determine the conserved sequence of the virus (the nucleic acid sequence is shown in SEQ ID NO: 4, and the protein sequence is shown in SEQ ID NO: 3),

[0052] 2. Compare the conservation of BALF4 gene base sequence and amino acid sequence between different Epstein-Barr virus strains ( figure 1 ), the results show that in Human gammaherpesvims 4, the similarity between the BALF4 sequence of different virus strains and the above-mentioned reference sequence is as high as 99%. Exon sequence, screening BALF3 antigen segment. Antibodies designed and prepared based on the segment sequence have relatively reliable detection effi...

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Abstract

The invention relates to the technical field of molecular biology and immunology, in particular to an EB virus BALF4 protein epitope. The invention provides an the EB virus BALF4 protein epitope. The EB virus BALF4 protein epitope is shown as SEQ ID NO: 1, provides a nucleic acid molecule for coding the epitope, and also provides an antigen protein, a specific antibody and application of the epitope. The epitope has good conservative property, sensitivity and specificity, and a prepared antibody is high in titer and can be applied to clinical diagnosis and molecular mechanism research.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and immunology, in particular to the antigenic epitope of Epstein-Barr virus BALF4 protein. Background technique [0002] Epstein-Barr virus (EBV) is a member of the genus Lymphotropia in the family Herpesviridae, and its genome is DNA. Epstein-Barr virus has the biological characteristics of specifically infecting human and some primate B cells in vivo and in vitro. Human is the host of Epstein-Barr virus infection, which is mainly transmitted through saliva. Asymptomatic infection mostly occurs in young children. More than 90% of children aged 3 to 5 have been infected with EB virus, and more than 90% of adults have virus antibodies. Epstein-Barr virus is the pathogen of infectious mononucleosis. In addition, Epstein-Barr virus is closely related to the occurrence of nasopharyngeal carcinoma and childhood lymphoma, and is listed as one of the human tumor viruses that may cause cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/05C07K16/08C12N15/38A61K39/42A61K39/245A61P31/22
CPCC07K14/005C07K16/085A61K39/12A61P31/22C12N2710/16222C07K2317/35Y02A50/30
Inventor 赵维莅熊杰
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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