Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof

A scorpion venom protein and colon-targeting technology, which is applied in the direction of peptide/protein components, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve obvious therapeutic effects, low production requirements, and simple production processes Effect

Inactive Publication Date: 2012-11-07
FUJIAN HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, no colon-targeting preparation containing scorpion venom has been seen in the domestic market, and there are no research reports on t

Method used

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  • Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof
  • Scorpion venom protein polypeptide colon-targeted preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Weigh 10 g of scorpion venom, and accurately weigh low-ester pectin according to the mass ratio of scorpion venom to low-ester pectin of 1:20 for later use. Sprinkle low-ester pectin into purified water in stages, dissolve to make a solution with a mass volume percentage concentration of 8%, add scorpion venom powder, stir to dissolve, and remove foam by ultrasonic vibration. Use a No. 6 injection needle to drop the mixed solution into a slowly stirred volume of 50 mL with a mass volume percentage concentration of 10% CaCl 2 In the solution, solidify for 30 minutes, filter, dry at 60°C, then soak the pellets in a PEI (polyethyleneimine) solution with a mass volume percentage concentration of 1.0%, crosslink for 6 hours, filter, wash with purified water, and dry at 60°C Dry. The colon-targeted preparation is obtained.

Embodiment 2

[0046] Weigh 5 g of scorpion venom, and accurately weigh low-ester pectin according to the mass ratio of scorpion venom to low-ester pectin of 1:10 for later use. Sprinkle low-ester pectin into purified water in stages, dissolve to make a solution with a mass volume percentage concentration of 6%, add scorpion venom powder, stir to dissolve, and remove foam by ultrasonic vibration. Use a No. 6 injection needle to drop the mixed solution into a slowly stirred volume of 50 mL with a mass volume percentage concentration of 8% CaCl 2 In the solution, solidify for 30 minutes, filter, and dry at 60°C, then soak the pellets in a PEI solution with a mass volume percentage concentration of 0.8%, crosslink for 6 hours, filter, wash with purified water, and dry at 60°C. The colon-targeted preparation is obtained.

Embodiment 3

[0048] Weigh 5 g of scorpion venom and 10 g of flufenamic acid (passed through an 80-mesh sieve), and accurately weigh the low-ester pectin according to the mass ratio of the drug to the low-ester pectin at 1:15 for future use. Sprinkle low-ester pectin into purified water in stages, dissolve to make a solution with a mass volume percentage concentration of 8%, add scorpion venom and flufenamic acid, stir and suspend evenly, and remove foam by ultrasonic vibration. Use a No. 6 injection needle to drop the mixed solution into a slowly stirred volume of 50 mL with a mass volume percentage concentration of 10% CaCl 2 In the solution, solidify for 30 minutes, filter, and dry at 60°C, then soak the pellets in a PEI solution with a mass volume percentage concentration of 1.0%, crosslink for 6 hours, filter, wash with purified water, and dry at 60°C. The colon-targeted preparation is obtained.

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Abstract

The invention discloses a scorpion venom protein polypeptide colon-targeted preparation and a preparation method thereof. Low-ester pectin is used as a main carrier and dripped into solution containing calcium ions, pellets are formed after reaction and solidification of the low-ester pectin, the pellets are soaked in polymine solution with a certain concentration and further cross-linked after being dried to form outer layers with higher hydrophobicity, and the scorpion venom protein polypeptide colon-targeted preparation is obtained after drying. After the preparation is taken orally, medicines are concentrated to release at the position of a colon, the concentration of the medicines at the position of the colon can be remarkably improved, the medicines directly contact with a focus of the colon and can be effectively prevented from being damaged by gastric acid or enzyme and the like in a stomach and a small intestine, and therapeutic effects of the medicines are made full use of.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a colon-targeting preparation of a scorpion venom polypeptide and a preparation method thereof. More specifically, the present invention relates to a pellet that can target the colon to release scorpion venom and stay in the colon for a long time and its preparation method. Background technique [0002] Scorpion venom is mainly composed of protein and non-protein. The main active ingredient of scorpion venom is protein, and the active protein is divided into toxic protein (scorpion toxin) and enzyme according to different functions. Scorpion toxin (Scor-pionvenom) is a kind of toxic protein composed of 20-80 amino acids containing C, H, O, N, S and other elements. The toxin has high specificity, high sulfur content, and a molecular weight of Between 6000 and 9000, but there are also about 3000 or more than 10000. The molecular structure of scorpion toxin contains 3 to 4...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61K38/17A61K47/48A61K47/36A61K47/38A61P1/00A61P1/04A61P7/02A61P25/04A61P29/02A61P35/00A61K31/196A61K31/606A61K31/665
Inventor 黄仁杰林国威叶榕肖健赖成敏
Owner FUJIAN HEALTH COLLEGE
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