95 results about "Scorpion Venoms" patented technology

The present invention is directed to recombinant human antibodies specific for Cn2 toxin from C. noxius scorpion venom. The antibodies are able to recognize the toxin and preferably neutralize it as well as the whole venom of C. noxius scorpion. This invention is also directed to a human non-immune phage display library. One clone that specifically binds the Cn2 toxin was affinity matured by directed evolution. Three cycles of maturation were performed and several scFv clones were isolated which specifically recognize toxin Cn2 with increased Kd of 446 fold. All variants were monomeric and only variants 6009F, 6105F and 6103E showed to be capable of neutralizing toxin Cn2 and the whole venom. Variant 6009F recognizes a different epitope than that of BCF2, a murine monoclonal antibody raised against scorpion toxin Cn2 which is also capable of neutralizing both Cn2 toxin and the whole venom when tested in mice, as well as that of commercially available polyclonal antibody fragments antivenom from horse. The scFv 6009F is the first reported recombinant human antibody fragment capable of neutralizing a scorpion venom. These results pave the way for the generation of safer autologous recombinant neutralizing antivenom against scorpion stings. The antibodies of the present invention can be used as part of a composition to treat those in need of treatment including those already stung by one or more scorpions, particularly C. noxius scorpions.

The invention relates to a scorpion venom polypeptide for promoting cell proliferation and a preparation method and medicinal application thereof. The scorpion venom polypeptide for promoting cell proliferation is characterized by being separated from the scorpion venom of Buthus martensii Karsch and then purified, 66 amino acid residues at the N end of the scorpion venom polypeptide for promoting cell proliferation are of a sequence shown in SEQ ID No. 1 in a sequence table and have molecular weight of 7217.4Da. The new polypeptide which is from the scorpion venom of Buthus martensii Karsch has a function of promoting the proliferation of hematopoietic cells and has the characteristics of single component, accurate molecular weight, clear 66 amino acid residues at the N end, obvious effect of promoting cell proliferation, controllable quality standard of the purification process; therefore the scorpion venom polypeptide for promoting cell proliferation is favorable to large-scale production. The scorpion venom polypeptide for promoting cell proliferation has significant advantages and wide application prospect in the aspect of development of medicines for promoting cell proliferation and preventing cell stress induced injury, radiation and the like and is capable of promoting the formation of the marrow hematopoietic cell colonies of mice subjected to Gamma-ray or X-ray irradiation and the proliferation of M-NFS-60 cells.

Various embodiments of this invention disclose a polarized dilute blue scorpion venom solution, a method for making a polarized dilute scorpion venom solution, and a method for administering dilute scorpion venom solution. The polarized dilute scorpion venom solution relieves pain, improves immune-system response, treats cancer, prevents cancer, improves quality of sleep, reduces inflammation, and minimizes negative biological response to chemotherapy and radiation treatment.