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Scorpion venom polypeptide for promoting cell proliferation and preparation method and medicinal application thereof

A technology of cell proliferation and scorpion venom, applied in the direction of extracellular fluid diseases, medical preparations containing active ingredients, peptide sources, etc., to achieve a significant effect of promoting cell proliferation, controllable quality standards, and a single ingredient effect

Inactive Publication Date: 2012-04-04
广州医学院
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  • Abstract
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Problems solved by technology

[0005] At present, there are many studies on the cell biological effect and mechanism of action of scorpion venom and its toxin (pure product of a single component) inhibiting cell growth at home and abroad, but for its promotion of cell proliferation and protection of cell stress damage (radiation, etc. ), there are no similar reports

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  • Scorpion venom polypeptide for promoting cell proliferation and preparation method and medicinal application thereof
  • Scorpion venom polypeptide for promoting cell proliferation and preparation method and medicinal application thereof
  • Scorpion venom polypeptide for promoting cell proliferation and preparation method and medicinal application thereof

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preparation example Construction

[0040] The preparation method of the scorpion venom polypeptide for promoting cell proliferation according to the present invention is characterized in that it comprises the following steps:

[0041] 1) Collect the venom of the East Asian pincer scorpion, extract fresh venom from the East Asian pincer scorpion, freeze-dry it into a gel by vacuum freeze-drying, and set aside;

[0042] 2) Separating the active component SVP IV of the scorpion venom polypeptide from the gel prepared in step 1), first perform gel filtration: use molecular sieve chromatography gel, that is, perform chromatography to separate peak II from the crude scorpion venom solution , collect peak II and freeze-dry it for later use; then carry out ion exchange and purification: use filler CM Sepharose FF to carry out ion exchange and further purification on the aforementioned peak II through an ion gel column, and use phosphate buffer solution of ph=6.4 as After gradient elution of the eluent, the effective co...

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Abstract

The invention relates to a scorpion venom polypeptide for promoting cell proliferation and a preparation method and medicinal application thereof. The scorpion venom polypeptide for promoting cell proliferation is characterized by being separated from the scorpion venom of Buthus martensii Karsch and then purified, 66 amino acid residues at the N end of the scorpion venom polypeptide for promoting cell proliferation are of a sequence shown in SEQ ID No. 1 in a sequence table and have molecular weight of 7217.4Da. The new polypeptide which is from the scorpion venom of Buthus martensii Karsch has a function of promoting the proliferation of hematopoietic cells and has the characteristics of single component, accurate molecular weight, clear 66 amino acid residues at the N end, obvious effect of promoting cell proliferation, controllable quality standard of the purification process; therefore the scorpion venom polypeptide for promoting cell proliferation is favorable to large-scale production. The scorpion venom polypeptide for promoting cell proliferation has significant advantages and wide application prospect in the aspect of development of medicines for promoting cell proliferation and preventing cell stress induced injury, radiation and the like and is capable of promoting the formation of the marrow hematopoietic cell colonies of mice subjected to Gamma-ray or X-ray irradiation and the proliferation of M-NFS-60 cells.

Description

technical field [0001] The invention relates to a scorpion venom polypeptide for promoting cell proliferation, a preparation method and a medicinal use thereof. It is a new polypeptide—Scorpion Venom Proliferation Peptide (Scorpion Venom) isolated from the venom of the East Asian scorpion (Buthus martensii Karsch). proliferative Peptide from Buthus martensii Karsch, BmKpp), the polypeptide can promote the proliferation of hematopoietic cells (normal / after radiation). Belongs to the field of biotechnology. Background technique [0002] There are 6 families, 70 genera, and more than 800 species of scorpions in the world. In China, the East Asian scorpion is the main species. The latter has historically been known both at home and abroad as one of the traditional and precious Chinese medicines in my country. Scorpion venom is a poisonous secretion secreted from the venom sac of the scorpion scorpion. It is the main effective part of the scorpion to treat diseases. Its curative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/18C07K1/16A61K38/17A61P7/06
Inventor 董伟华孔天翰王燕郑跃钱邱轶芳王彩霞蒋丽苑李婷邢百倩
Owner 广州医学院
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