54results about How to "Activity is not destroyed" patented technology

The invention relates to a method for preparing anti-influenza virus transfer factor, comprising following steps: preparing raw material, pre-treating, disintegrating, freezing and thawing, extracting, ultra-filtering, disinfecting and packing. The invention employs process of first immune and second extraction. The preparation time is reduced because of low-temperature freezing and thawing and hollow fiber tube ultra-filtering, so the substance activity will not be destroyed. It can be made into oral liquid.

The invention discloses a pericarp ferment mask and a preparation method thereof. The pericarp ferment mask comprises a mask cloth and a mask liquid. The mask cloth is immersed in the mask liquid. The mask liquid comprises, by weight, 4-6 parts of a pericarp ferment stock solution, 4-6 parts of mucopolysaccharide, 3-5 parts of glycerin, 3-4 parts of an emulsifier, 2-4 parts of arbutin, 2-4 parts of mushroom glucan, 1-3 parts of hyaluronic acid, 1-3 parts of collagen peptide powder, 1-2 parts of vitamin E, 4-6 parts of propylene glycol, 1-1.5 parts of a thickening agent, 0.4-0.8 parts of dipotassium glycyrrhizinate, 0.1-0.3 parts of an antiseptic, 0.05 parts of essence and 50-70 parts of deionized water. The preparation method creatively uses a pericarp ferment in cosmetics. The pericarp ferment is rich in naringin and a plurality of vitamins. Through natural fermentation of pericarp, active ingredients of the pericarp are fully dissolved out and can be absorbed easily by skin.

The present invention relates to a nostoc sphaeroids kutz sweetmeat and a production method thereof, wherein the nostoc sphaeroids kutz sweetmeat is prepared from the following raw materials by weight: 50-70 parts of fresh nostoc sphaeroids kutz, 25-45 parts of dendrobium candidum, 5-10 parts of nostoc sphaeroids kutz polysaccharide dry powder, 10-20 parts of fruit juice powder, and 10-25 parts of white sugar or rock candy. The production method comprises: decocting dendrobium candidum and nostoc sphaeroids kutz polysaccharide dry powder to obtain an extraction liquid; adding fruit juice powder and white sugar or rock candy to the extraction liquid according to a certain ratio, uniformly mixing, adding fresh nostoc sphaeroids kutz being subjected color protection hardening, and impregnating; and drying at a temperature 35-40 DEG after completing the impregnating so as to obtain the product. According to the present invention, the nostoc sphaeroids kutz sweetmeat has advantages of complete nutrition and excellent taste, and has health functions of invigoration, health enhancing, aging resistance, oxidation resistance, antitumor, and the like; and the production method is simple and easy to perform, and the activities of the phycobiliprotein and the polysaccharide of the nostoc sphaeroids kutz cannot be damaged to the greatest extent.

The invention relates to the food field, in particular to a nostoc sphaeroids kutz jelly. The nostoc sphaeroids kutz jelly comprises the following components in percentage by weight: 15 to 25 percentage of rough extract of nostoc sphaeroids kutz phycobiliprotein, 0.1 to 0.5 percentage of dry polysaccharide powder, 10 to 20 percentage of juice, 0.03 to 0.2 percentage of edible gum, 10 to 13 percentage of honey, 0.05 to 0.2 percentage of citric acid, 4 to 5 percentage of xylitol, 0.01 to 0.03 percentage of potassium sorbate, 0.01 to 0.05 percentage of emulsifier, 0.01 to 0.1 percentage of solid cream, 0.1 to 0.2 percentage of vanillin, and the balance of water. The nostoc sphaeroids kutz jelly disclosed by the invention not only has bright color and brings fresh and pleasure sensation to human vision, but also has the function of oxidation resistance and enhances human immunity. Through the compound use of the nostoc sphaeroids kutz gel nutrient solution and edible gel, the dosage of the edible gel is reduced, and the jelly is better in taste.

This invention relates to preparation method and equipment for nano-level biological-power raw material which comprises, employing microwave to assist abstraction of continual flow liquid at low temperature, condensing thin membrane and decompressing, and carrying out low temperature supersonic jet spray drying; fit to large range material, and can make obtain needed constituent activity with no damage.

The invention relates to the technical field of medicine, in particular to a cryoprotectant and a lyophilization method for a latex coupled antibody. The cryoprotectant comprises BSA (bovine serum albumin), PEG (polyethylene glycol) and PVP (polyvinylpyrrolidone). The cryoprotectant is designed for the latex coupled antibody and can protect the activity of the latex coupled antibody from being damaged in a lyophilization process in combination with the provided lyophilization method, non-specific aggregation in a redissolution process after lyophilization cannot happen, and the antibody titer keeps stable after redissolution. Repeated experiments prove that the deviation is not obvious, the relative deviation is plus or minus 10% or smaller, and the cryoprotectant has good lyophilization protection effect.

The invention discloses a preparation method for transferring gene of a natural effective pure biological active matter for resisting a newcastle disease virus. The invention is carried out according to the following steps: inoculating the newcastle disease virus into a chick embryo allantois liquid of ten days to prepare a virus antigen; extracting the virus antigen; using the extracted virus antigen for immunizing a pig; extracting the transferring gene for resisting the newcastle disease virus from the immunized pig; therefore, the transferring gene of the invention can be used for playing the role of preventing the infection of the newcastle disease virus and protecting a normal organism cell from being infected by the newcastle disease virus, thereby reducing the disease rate.

The invention discloses a method for extracting herbaceous plants for external application by using small molecule cutting technology. The method comprises the following steps: crushing herbaceous plants, adding a first solvent into the crushed herbaceous plants for grinding so as to obtain slurry, filtering the slurry and recovering a filtrate; separating the first solvent and a solute in the recovered filtrate and subjecting the separated solute to micro laser irradiation; and dissolving the irradiated solute in a second solvent to form a solution, successively stirring and standing the solution, separating the second solvent from a solute and recovering the solute which is an element-level active component. Through the micro laser irradiation and cutting of the solute after separation of the first solvent, the solute reaches the element level and maintains its activity and efficacy.

The invention discloses toner and a method for manufacturing the same. The toner comprises fruit peel enzyme stock solution, glycol distearate, decyl polyglucoside, sorbitol and water. The toner and the method have the advantages that fruit peel enzymes are creatively applied to cosmetics, fruit peel contains abundant naringin and diversified vitamins, and accordingly effective ingredients can be sufficiently dissolved after the fruit peel is naturally fermented, and can be easily absorbed by the skins of users.

The invention relates to a method for separating and purifying bromelain by circulating an aqueous two-phase system, and belongs to the technical field of separation and purification of effective ingredients of natural products. The method comprises the following steps: firstly, pretreating to obtain coarsely extracted pineapple juice; then, extracting bromelain for the first time by an aqueous two-phase system, detecting the activity and protein content of top-phase bromelain, and determining an extraction effect; thirdly, taking out polymer, and diluting by adding a proper amount of distilled water, regulating the pH value of the solution, and extracting bromelain for the second time by a cloud point method; and finally, taking the separated polymer for circulating extraction experiments in the aqueous next two-phase system. The method has a simple process flow, low cost and mild operation condition and can be used for protecting the structure and activity of target enzyme; and the polymer can be recycled, and has small environmental pollution.

The invention discloses a fish cartilage extract for preventing arthritis. The fish cartilage extract is prepared from 80-90% of low-molecular-weight chondroitin sulfate and 10-20% of collagen peptide, and the molecular weight of the low-molecular-weight chondroitin sulfate is 400-500 Da. A preparation method of the fish cartilage extract includes the following steps: fish cartilages are soaked indistilled water and then boiled, fish meat and grease are removed, drying is conducted, and superfine grinding is conducted to obtain fish cartilage powder; distilled water and a complex enzyme are added into the fish cartilage powder, enzymolysis, enzyme killing, centrifugation and ultra-filtration are conducted, and then drying is conducted to obtain ultra-filtration powder; and distilled waterand a chondroitin sulfate AC enzyme are added into the ultra-filtration powder for enzymatic degradation, enzyme killing, filtration, ultra-filtration, concentration and dialysis are conducted, and freeze drying is conducted to obtain the fish cartilage extract. The fish cartilage extract has the beneficial effects that the fish cartilage extract has the effect of anticomplement activation, can protect chondrocytes against being cracked and has the better potential curative effect in prevention and treatment of arthritis.

The invention discloses a separation and purification method of silkworm pupa protein polypeptide. The separation and purification method comprises the following specific steps: dissolving a silkwormpupa protein enzymatic hydrolysate into double distilled water and centrifuging to obtain a supernatant, namely silkworm pupa protein polypeptide raw liquid; dissolving the silkworm pupa protein polypeptide liquid into the double distilled water, centrifuging, adding a supernatant into an ion exchange resin chromatography column, eluting and collecting protein polypeptide which has optimum inhibitory activity on ACE, namely an ion exchange chromatography enzymatic hydrolysate; dissolving the ion exchange chromatography enzymatic hydrolysate into the double distilled water, centrifuging, carrying out sephadex column chromatographic separation on the supernatant, eluting with the double distilled water and collecting elution components according to an absorbance curve at 280nm; dissolving the gel chromatography enzymatic hydrolysate into the double distilled water and purifying by using high performance liquid chromatography, thus obtaining the silkworm pupa protein polypeptide. The separation and purification method of the silkworm pupa protein polypeptide, disclosed by the invention, has the beneficial effects that the separation method is simple and the protein polypeptide can beseparated quickly and efficiently; the protein polypeptide has the advantages of high yield, low loss, high purity and no possibility of destroying the activity; the obtained protein polypeptide has good inhibitory activity on the ACE.

The invention discloses an extraction process for active ingredients of pericarpium trichosanthis, and relates to the technical field of pericarpium trichosanthis processing. The process includes thesteps of cleaning, freezing, extracting polysaccharides, extracting alkaloids and extracting triterpenoid saponins, cleaning process is carried out by using magnesium sulfate and molybdenum nitrate for cleaning, freezing is to freeze pericarpium trichosanthis powder at 10 DEG C for 3-4h, after thawing, cellulase, citric acid, tert-butyl hydroquinone and diallyl isophthalate extract polysaccharideare added, centrifuge sediment is added to acid and alkali-dissolving alkaloids and stored in liquid nitrogen, activated carbon is added for adsorption, and triterpenoid saponins is added to acidic extracts of celestial and sucrose for extraction; the process can be used for sequentially extracting polysaccharides, alkaloids and the triterpenoids, the extraction process is simple and easy to operate, the production efficiency is high, the yield of extracts is high, and the purity is high, thereby achieving the effective utilization of the value of the pericarpium trichosanthis.

The invention discloses a dietotherapy nutrient solid beverage used for nephrosis and a preparation method thereof. Effective components of the solid beverage comprise 1.5-2.0 parts by weight of yam,0.9-1.4 parts by weight of rhizoma polygonati, 0.9-1.4 parts by weight of fructus rubi, 0.9-1.4 parts by weight of semen euryales and 1.5-2.0 parts by weight of fresh rhizoma imperatae. The productionmethod comprises the steps that the four traditional Chinese medicines of the yam, the rhizoma polygonati, the fructus rubi and the semen euryales are taken, mixed according to a ratio, crushed intocoarse powder, and screened by a 10-mesh screen, water with weight 10 times of the total weight of the yam, the rhizoma polygonati, the fructus rubi and the semen euryales is added, decoction and extraction are conducted twice, each extraction is maintained for 45 minutes after boiling, filter liquid is combined, concentrated to form thick paste at low temperature, and subjected to decompression drying to obtain dried paste, and crushing is conducted to obtain extract powder 1; the fresh rhizoma imperatae is taken, and is cut into segments, 70% ethyl alcohol is added, low-temperature extraction is conducted for 4 hours, 50 times amount of ethyl alcohol is used for percolation, percolate is subjected to decompression to recover the ethyl alcohol until no alcohol smells exist, decompressiondrying is conducted continuously to obtain dried paste, and crushing is conducted to obtain extract powder 2. The extract powder 1 and the extract powder 2 are mixed evenly according to a weight ratioof 1 to 1, and are screened, and bagging is conducted to obtain a finished product.

The invention relates a heating device for a wastewater biochemical pool. The heating device comprises a heat absorption section, a heat insulation section and a heat dissipation section which are internally communicated and sequentially connected; the heat absorption section and the heat insulation section are vertically tubular; the heat dissipation section is a hollow horizontal plate; the heat absorption section is embedded in ground; and the heat dissipation section is positioned in the wastewater biochemical pool. The heat absorption section of the heating device is heated by using terrestrial heat so that a working medium in a heater is boiled to be at vapor state and enter the heat dissipation section through the heat insulation section, the temperature of wastewater in the biochemical pool is raised, and the activity of biochemical bacteria is substantially increased.

The invention discloses lipid-decreasing functional peptide by using silkworm chrysalis as raw material. The molecular weight of the lipid-decreasing functional peptide is 620 to 1200Da. A preparationmethod of the functional peptide comprises the following steps of pretreatment of silkworm chrysalis powder: adding a mixed solution of n-hexane and petroleum ether into the silkworm chrysalis powder, heating and refluxing, so as to obtain silkworm chrysalis protein; adding distilled water into the silkworm chrysalis protein powder, uniformly mixing, adding alkaline protease and papain, then adding D-carnitine, hydrolyzing by enzyme, deactivating enzyme, cooling, and then centrifuging, so as to obtain a supernatant for further use, namely an enzyme hydrolysis liquid; filtering the enzyme hydrolysis liquid by an ultrafiltration film, so as to obtain an ultrafiltration enzyme hydrolysis liquid; sequentially performing gel column chromatography, ion exchange resin chromatography, and high performance liquid chromatography purification on the ultrafiltration enzyme hydrolysis liquid, so as to obtain the lipid-decreasing functional peptide. The lipid-decreasing functional peptide has the beneficial effects that most of lipid forming genes can be regulated down, the stacking of lipid is decreased, the lipid-decreasing function is realized, and the lipid-decreasing functional peptide canbe developed into lipid-decreasing medicines, health-care products and food additives.

The invention discloses a preparation method of silkworm pupa protein polypeptide. The preparation method comprises the following specific steps: adding a mixed solution of n-hexane and petroleum ether into silkworm pupa meal and carrying out heating reflux to obtain silkworm pupa protein; dissolving a silkworm pupa protein enzymatic hydrolysate into double distilled water, centrifuging to obtaina supernatant, namely silkworm pupa protein polypeptide raw liquor; adding distilled water into the silkworm pupa meal, uniformly mixing and adjusting a pH value; then adding alkaline protease and neutral protease, carrying out enzymolysis, enzyme deactivation and centrifuging, and concentrating the supernatant, namely the silkworm pupa protein polypeptide raw liquor; carrying out separation and purification on the silkworm pupa protein polypeptide raw liquor through ion-exchange resin chromatographic column, gel column chromatography and high performance liquid chromatography, thus obtainingthe silkworm pupa protein polypeptide. The preparation method of the silkworm pupa protein polypeptide, disclosed by the invention, has the beneficial effects of simplicity and feasibility; the protein polypeptide has the advantages of high yield, low loss, high purity and no possibility of destroying the activity; the obtained protein polypeptide has good inhibitory activity to ACE.

The invention relates to a production method and an application of an immune globulin G. Specifically, the production method comprises steps of: carrying out whole blood anticoagulating, centrifugal separation, diluting, FeCl3 precipitation reaction, clarifying, centrifugal separation, nano filtration and desalination, embedding, spray-drying, etc. The production method disclosed by the invention not only solves the problems of resource waste, environmental pollution and the like of animal byproduct pig blood, but also greatly improves the utilization value of the pig blood through continuous mass production processing process; the prepared immune globulin G, through an enzyme-linked immuno sorbent assay (ELISA) activity identification, is greater than 90% in activity, and has a special treating effect on intestinal diseases of small animals; and the immune globulin G can be used as an immuno potentiator for a weak animal group and as normal nutrition supplementary for the body.

The invention discloses a preparation method of silkworm chrysalis collagen. The preparation method comprises the following concrete steps: adding a degreasing agent into silkworm chrysalis meal for degreasing, carrying out suction filtration, and drying filter residues; adding a phosphate buffer solution and neutral protease into the degreased silkworm chrysalis, carrying out enzymolysis and enzyme deactivation, and cooling and then centrifuging to obtain supernatant, i.e., crude protein liquid; adding a chitosan solution into the crude protein liquid, stirring, centrifuging supernatant and putting into an ice-water bath, adding ammonium sulfate, stirring until complete dissolution, standing overnight, centrifuging, dissolving precipitate in the phosphate buffer, carrying out dialysis, enabling dialyzate to enter a hydroxyapatite column body, adding a buffer solution for eluting, and freeze-drying to obtain the silkworm chrysalis collagen. The preparation method of silkworm chrysaliscollagen is simple and feasible, and high in degreasing efficiency; the collagen is high in yield, purity and activity, and has more concentrated molecular weight; the method is suitable for industrial production and higher in safety, and increases the additional value of the silkworm chrysalis.

The invention discloses a continuous extraction method of proteins and meal fibers from walnut meal. The method includes steps of carrying out a microwave drying process to walnut meal for 30min-40min with a vacuum degree being 60kpa-80kpa and a power being 100kW-200kW; after an ultramicro crushing and screening process, extracting walnut proteins from the walnut meal in a manner of a combination of an alkaline-microwave extraction process with a freeze drying process; carrying out a single-step degradation process to a precipitate through a compound enzyme and performing alcohol-precipitation and centrifugation; and carrying out an air-flow spray drying process to obtain water-soluble meal fibers and water-insoluble meal fibers with the air-inlet temperature being 120-180 DEG C, the air-outlet temperature being 60-80 DEG C and the compressed-air flow being 500-800 mL / h. Through the method, the dissolution rate of walnut proteins is increased, activity and nutritive value of the proteins is maintained and enzymatic hydrolysis efficiency and purity and yield of the meal fibers are increased. Recycling of waste materials is achieved and problems of wasting resource and polluting environment are solved.

The invention discloses special pine-nut-protein-peptide-enriched antioxidant lozenges for astronauts and a preparation method thereof. The special pine-nut-protein-peptide-enriched antioxidant lozenges for the astronauts are prepared from the following raw materials and auxiliary materials: 20-40% of pine nut protein peptides, 10-20% of blueberry anthocyanin dry-powder, 3-6% of raspberry powder,1-2% of beta-carotene, 2-4% of taurine, 1-2% of vitamin C, 1-2% of vitamin E, 1-2% of alpha-lipoic acid, 8-15% of beta-cyclodextrin, 2-5% of buckwheat starch, 10-15% of xylitol, 2-4% of citric acid, 5-10% of fructo-oligosaccharides, and 2-4% parts of grape powder. The preparation method of the special pine-nut-protein-peptide-enriched antioxidant lozenges for the astronauts is characterized in that the pine nuts with high protein content are taken as raw materials, and the raw materials and the auxiliary materials are proportioned so as to ensure balanced nutrition; and thus, the prepared pine-nut-protein-peptide-enriched antioxidant lozenges have the characteristics of resistance to high-energy particle radiation in the space, small volume, and good taste. The pine-nut-protein-peptide-enriched antioxidant lozenges can be used for activities of astronauts in space stations, aviation pilots, field forces, navigation, submarines and so on. Moreover, the pine-nut-protein-peptide-enrichedantioxidant lozenges can be also used for people with the demands of preventing cardiovascular and cerebrovascular diseases as well as diabetes syndromes, and improving intestinal flora, reducing blood lipid and lowering blood pressure.

The invention belongs to the technical field of preparation of feed additives and particularly relates to a preparation method and application of a compound Chinese herbal medicine preparation for sheep. The preparation method comprises the steps as follows: 100-150 g of high-quality radix astragali, 60-120 g of medicated leaven, 60-120 g of malt, 60-120 g of haws, 60-120 g of dried orange peel, 60-120 g of leaves of eucommia ulmoides, 100-150 g of officinal magnolia bark, 100-200 g of houttuynia cordata, 40-80 g of garlic, 40-80 g of ginger and 60-120 g of tarragon are thoroughly cleaned anddried, the obtained materials are crushed into 60-mesh powder, ultrasonic extraction is performed twice in the ratios of medicines to water (by weight) being 1:30 and 1:20 respectively at 60 DEG C atultrasonic power of 1250 W for 60 min, extraction liquids are mixed and concentrated into extract of 4.25-5.55 (25 DEG C), the extract is mixed with calcium lactate in the ratio being 1:5, and drying,crushing, sieving and uniform mixing are performed to prepare the preparation.

The invention belongs to the field of daily chemicals. The invention discloses foundation cream, which comprises the following components: cyclopentasiloxane, glycerol, isononyl isononanoate, titanium dioxide, bismuth oxychloride, ferric oxide, aluminum oxide, silica, triethoxyoctyl silane, polydimethylsiloxane, an emulsifier, mica, disteardimonium hectorite, polymethyl methacrylate, sodium chloride, panthenol, magnesium stearate, butanediol, a plant extract and the balance of water. The foundation cream is fine and smooth in cream body, light and thin in texture, good in ductility, smooth to smear, easy to make up, natural and fit in makeup face, free of powder floating, fresh, non-sticky, breathable, free of pore blocking, high in covering power, capable of hiding pores, adjusting the skin color, brightening the skin color, controlling oil and moisturizing for a long time, nourishing and moistening the skin, waterproof, sweat-proof, ultraviolet-proof and good in makeup maintaining effect.

The invention discloses a small molecular weight silkworm pupa collagen with a molecular weight of 600-1000Da. A preparation method of the collagen is as follows: adding a degreasing agent to silkwormpupa powder for degreasing, filtering by suction, and drying filter residue; adding a phosphate buffer liquid and neutral protease to the degreased silkworm pupa for enzymatic hydrolysis, performingenzyme deactivation, and centrifuging after cooling to obtain supernatant, namely a crude protein solution; adding a chitosan solution to the crude protein solution, stirring, centrifuging, putting supernatant into an ice water bath, adding ammonium sulfate, stirring until the ammonium sulfate is completely dissolved, standing overnight, centrifuging, dissolving a precipitate in a phosphate buffer, dialyzing, allowing dialysate into a hydroxyapatite column, adding the buffer to elute, and freezing-drying to obtain the small molecular weight silkworm pupa collagen. The beneficial effects are asfollows: the small molecular weight silkworm pupa collagen has high purity, high activity, and relatively-concentrated molecular weight, and the preparation method is simple and feasible, high in degreasing efficiency, high in yield, easy in industrial production and higher in safety and improves the added value of silkworm pupa.

The invention belongs to the technical field of preparation of traditional Chinese medicine preparations for livestock, in particular discloses a method for preparing a compound radix codonopsis preparation composition for sheep, and further relates to application of the preparation composition in a compound feed for sheep and application in an additive premixed feed for sheep. The method disclosed by the invention comprises the following steps: selecting 100-200g of high-quality radix codonopsis, 80-150g of rhizoma atractylodis macrocephalae, 50-100g of rhizoma chuanxiong, 50-100g of fructusligustri lucidi, 50-100g of fructus psoraleae, 50-100g of radix et rhizoma rhei, 50-100g of fructus cnidii, 50-100g of licorice roots, 50-100g of rhizoma atractylodis and 50-100g of seaweeds, performing cleaning, performing drying, crushing the components into powder of 60 meshes, performing ultrasonic extraction on the powder for 70 minutes by using 65% ethanol with an amount being 30 times of the amount of the powder at ultrasonic power of 900W at an ultrasonic temperature of 50 DEG C, further performing ultrasonic extraction on medicine dregs for 60 minutes by using water with an amount being 10 times of the amount of the medicine dregs at ultrasonic power of 1200W at an ultrasonic temperature of 60 DEG C, performing concentration so as to obtain an extract of which the relative densityis 3.55-4.55 at 25 DEG C, uniformly mixing the extract with hippophae rhamnoides fruit dreg powder in a ratio of 1:10, performing pelletizing, and performing drying, so as to obtain the compound radix codonopsis preparation composition.

The invention discloses a method for separating and purifying silkworm chrysalis protein polypeptides. The specific steps of the separation and purification method are: dissolving silkworm chrysalis protein enzymatic hydrolyzate in double-distilled water, centrifuging the supernatant to become silkworm chrysalis protein polypeptide crude liquid; dissolving silkworm chrysalis protein peptide liquid Soluble in double distilled water, add the supernatant to the ion exchange resin chromatography column after centrifugation, elute, and collect the protein polypeptide with the best ACE inhibitory activity, which is the ion exchange chromatography enzymatic hydrolyzate; the ion exchange chromatography enzyme The hydrolyzate was dissolved in double distilled water, and after centrifugation, the supernatant was separated by Sephadex column chromatography, eluted with double distilled water, and the eluted components were collected according to the absorbance curve at 280nm; the above gel chromatography enzymatic hydrolyzate was dissolved Purified in double distilled water by high performance liquid chromatography to obtain the silkworm chrysalis protein polypeptide. The beneficial effects are: the separation method of the present invention is simple, and the protein polypeptide can be separated quickly and efficiently, with high yield, low loss, high purity, and undamaged activity of the protein polypeptide, and the obtained protein polypeptide has good ACE inhibitory activity.

The present invention discloses applications of sea sedge capable of lowering blood pressure, and applications to health-care foods, foods or feeds. The sea sedge is prepared by the following steps: raw material mixing, first baking, seasoning, secondary baking, slicing and packaging. The beneficial effects of the sea sedge are as follows: through comparison, the sea sedge capable of lowering blood pressure and obtained by the invention has little difference with market products in luster, tastes and impurity content, but is more fluffy and uniform, and neat in slices and uniform in thickness.After twice baking, the flavor of fat twice is better, and the sea sedge has a unique mouth feel. In addition, the sea sedge obtained by the invention has higher nutritional value, and has the effects of lowering blood pressure and protecting cardiac muscles.

The invention discloses an extraction method and application of peony leaf rachis crude polysaccharide. The extraction method of the peony leaf rachis crude polysaccharide comprises the following steps: step 1): pre-treating raw materials; step 2): carrying out extraction and separation on leaf rachis powder obtained by step 1) and collecting separated supernatant to obtain the peony leaf rachis crude polysaccharide, wherein the extraction and separation step is carried out according to the following manner: uniformly mixing the leaf rachis powder obtained by step 1) and an extraction agent according to the liquid / material ratio of (8 to 28)ml to 1g; extracting for 10 to 60min by utilizing an ultrasonic extractor under the conditions that the temperature is 20 to 70 DEG C and the ultrasonic power is 100 to 300W; filtering to obtain filtering dreg and a water extraction solution; centrifuging the water extraction solution and taking supernatant, wherein the supernatant is a peony leaf rachis crude polysaccharide solution. According to the extraction method disclosed by the invention, the raw materials are selected from peony leaf rachises which are originally discarded as wastes, and plant resources are sufficiently utilized; the extraction method has the advantages that an operation method flow is simple and easy to realize and the extraction efficiency is relatively high.

The invention relates to a method for separating and purifying bromelain by circulating an aqueous two-phase system, and belongs to the technical field of separation and purification of effective ingredients of natural products. The method comprises the following steps: firstly, pretreating to obtain coarsely extracted pineapple juice; then, extracting bromelain for the first time by an aqueous two-phase system, detecting the activity and protein content of top-phase bromelain, and determining an extraction effect; thirdly, taking out polymer, and diluting by adding a proper amount of distilled water, regulating the pH value of the solution, and extracting bromelain for the second time by a cloud point method; and finally, taking the separated polymer for circulating extraction experiments in the aqueous next two-phase system. The method has a simple process flow, low cost and mild operation condition and can be used for protecting the structure and activity of target enzyme; and the polymer can be recycled, and has small environmental pollution.