54results about How to "Activity is not destroyed" patented technology

The invention discloses a pericarp ferment mask and a preparation method thereof. The pericarp ferment mask comprises a mask cloth and a mask liquid. The mask cloth is immersed in the mask liquid. The mask liquid comprises, by weight, 4-6 parts of a pericarp ferment stock solution, 4-6 parts of mucopolysaccharide, 3-5 parts of glycerin, 3-4 parts of an emulsifier, 2-4 parts of arbutin, 2-4 parts of mushroom glucan, 1-3 parts of hyaluronic acid, 1-3 parts of collagen peptide powder, 1-2 parts of vitamin E, 4-6 parts of propylene glycol, 1-1.5 parts of a thickening agent, 0.4-0.8 parts of dipotassium glycyrrhizinate, 0.1-0.3 parts of an antiseptic, 0.05 parts of essence and 50-70 parts of deionized water. The preparation method creatively uses a pericarp ferment in cosmetics. The pericarp ferment is rich in naringin and a plurality of vitamins. Through natural fermentation of pericarp, active ingredients of the pericarp are fully dissolved out and can be absorbed easily by skin.

The present invention relates to a nostoc sphaeroids kutz sweetmeat and a production method thereof, wherein the nostoc sphaeroids kutz sweetmeat is prepared from the following raw materials by weight: 50-70 parts of fresh nostoc sphaeroids kutz, 25-45 parts of dendrobium candidum, 5-10 parts of nostoc sphaeroids kutz polysaccharide dry powder, 10-20 parts of fruit juice powder, and 10-25 parts of white sugar or rock candy. The production method comprises: decocting dendrobium candidum and nostoc sphaeroids kutz polysaccharide dry powder to obtain an extraction liquid; adding fruit juice powder and white sugar or rock candy to the extraction liquid according to a certain ratio, uniformly mixing, adding fresh nostoc sphaeroids kutz being subjected color protection hardening, and impregnating; and drying at a temperature 35-40 DEG after completing the impregnating so as to obtain the product. According to the present invention, the nostoc sphaeroids kutz sweetmeat has advantages of complete nutrition and excellent taste, and has health functions of invigoration, health enhancing, aging resistance, oxidation resistance, antitumor, and the like; and the production method is simple and easy to perform, and the activities of the phycobiliprotein and the polysaccharide of the nostoc sphaeroids kutz cannot be damaged to the greatest extent.

The invention relates to the food field, in particular to a nostoc sphaeroids kutz jelly. The nostoc sphaeroids kutz jelly comprises the following components in percentage by weight: 15 to 25 percentage of rough extract of nostoc sphaeroids kutz phycobiliprotein, 0.1 to 0.5 percentage of dry polysaccharide powder, 10 to 20 percentage of juice, 0.03 to 0.2 percentage of edible gum, 10 to 13 percentage of honey, 0.05 to 0.2 percentage of citric acid, 4 to 5 percentage of xylitol, 0.01 to 0.03 percentage of potassium sorbate, 0.01 to 0.05 percentage of emulsifier, 0.01 to 0.1 percentage of solid cream, 0.1 to 0.2 percentage of vanillin, and the balance of water. The nostoc sphaeroids kutz jelly disclosed by the invention not only has bright color and brings fresh and pleasure sensation to human vision, but also has the function of oxidation resistance and enhances human immunity. Through the compound use of the nostoc sphaeroids kutz gel nutrient solution and edible gel, the dosage of the edible gel is reduced, and the jelly is better in taste.

This invention relates to preparation method and equipment for nano-level biological-power raw material which comprises, employing microwave to assist abstraction of continual flow liquid at low temperature, condensing thin membrane and decompressing, and carrying out low temperature supersonic jet spray drying; fit to large range material, and can make obtain needed constituent activity with no damage.

The invention relates to the technical field of medicine, in particular to a cryoprotectant and a lyophilization method for a latex coupled antibody. The cryoprotectant comprises BSA (bovine serum albumin), PEG (polyethylene glycol) and PVP (polyvinylpyrrolidone). The cryoprotectant is designed for the latex coupled antibody and can protect the activity of the latex coupled antibody from being damaged in a lyophilization process in combination with the provided lyophilization method, non-specific aggregation in a redissolution process after lyophilization cannot happen, and the antibody titer keeps stable after redissolution. Repeated experiments prove that the deviation is not obvious, the relative deviation is plus or minus 10% or smaller, and the cryoprotectant has good lyophilization protection effect.

The invention discloses a preparation method for transferring gene of a natural effective pure biological active matter for resisting a newcastle disease virus. The invention is carried out according to the following steps: inoculating the newcastle disease virus into a chick embryo allantois liquid of ten days to prepare a virus antigen; extracting the virus antigen; using the extracted virus antigen for immunizing a pig; extracting the transferring gene for resisting the newcastle disease virus from the immunized pig; therefore, the transferring gene of the invention can be used for playing the role of preventing the infection of the newcastle disease virus and protecting a normal organism cell from being infected by the newcastle disease virus, thereby reducing the disease rate.

The invention discloses a method for extracting herbaceous plants for external application by using small molecule cutting technology. The method comprises the following steps: crushing herbaceous plants, adding a first solvent into the crushed herbaceous plants for grinding so as to obtain slurry, filtering the slurry and recovering a filtrate; separating the first solvent and a solute in the recovered filtrate and subjecting the separated solute to micro laser irradiation; and dissolving the irradiated solute in a second solvent to form a solution, successively stirring and standing the solution, separating the second solvent from a solute and recovering the solute which is an element-level active component. Through the micro laser irradiation and cutting of the solute after separation of the first solvent, the solute reaches the element level and maintains its activity and efficacy.

The invention discloses a fish cartilage extract for preventing arthritis. The fish cartilage extract is prepared from 80-90% of low-molecular-weight chondroitin sulfate and 10-20% of collagen peptide, and the molecular weight of the low-molecular-weight chondroitin sulfate is 400-500 Da. A preparation method of the fish cartilage extract includes the following steps: fish cartilages are soaked indistilled water and then boiled, fish meat and grease are removed, drying is conducted, and superfine grinding is conducted to obtain fish cartilage powder; distilled water and a complex enzyme are added into the fish cartilage powder, enzymolysis, enzyme killing, centrifugation and ultra-filtration are conducted, and then drying is conducted to obtain ultra-filtration powder; and distilled waterand a chondroitin sulfate AC enzyme are added into the ultra-filtration powder for enzymatic degradation, enzyme killing, filtration, ultra-filtration, concentration and dialysis are conducted, and freeze drying is conducted to obtain the fish cartilage extract. The fish cartilage extract has the beneficial effects that the fish cartilage extract has the effect of anticomplement activation, can protect chondrocytes against being cracked and has the better potential curative effect in prevention and treatment of arthritis.

The invention discloses a separation and purification method of silkworm pupa protein polypeptide. The separation and purification method comprises the following specific steps: dissolving a silkwormpupa protein enzymatic hydrolysate into double distilled water and centrifuging to obtain a supernatant, namely silkworm pupa protein polypeptide raw liquid; dissolving the silkworm pupa protein polypeptide liquid into the double distilled water, centrifuging, adding a supernatant into an ion exchange resin chromatography column, eluting and collecting protein polypeptide which has optimum inhibitory activity on ACE, namely an ion exchange chromatography enzymatic hydrolysate; dissolving the ion exchange chromatography enzymatic hydrolysate into the double distilled water, centrifuging, carrying out sephadex column chromatographic separation on the supernatant, eluting with the double distilled water and collecting elution components according to an absorbance curve at 280nm; dissolving the gel chromatography enzymatic hydrolysate into the double distilled water and purifying by using high performance liquid chromatography, thus obtaining the silkworm pupa protein polypeptide. The separation and purification method of the silkworm pupa protein polypeptide, disclosed by the invention, has the beneficial effects that the separation method is simple and the protein polypeptide can beseparated quickly and efficiently; the protein polypeptide has the advantages of high yield, low loss, high purity and no possibility of destroying the activity; the obtained protein polypeptide has good inhibitory activity on the ACE.

The invention discloses lipid-decreasing functional peptide by using silkworm chrysalis as raw material. The molecular weight of the lipid-decreasing functional peptide is 620 to 1200Da. A preparationmethod of the functional peptide comprises the following steps of pretreatment of silkworm chrysalis powder: adding a mixed solution of n-hexane and petroleum ether into the silkworm chrysalis powder, heating and refluxing, so as to obtain silkworm chrysalis protein; adding distilled water into the silkworm chrysalis protein powder, uniformly mixing, adding alkaline protease and papain, then adding D-carnitine, hydrolyzing by enzyme, deactivating enzyme, cooling, and then centrifuging, so as to obtain a supernatant for further use, namely an enzyme hydrolysis liquid; filtering the enzyme hydrolysis liquid by an ultrafiltration film, so as to obtain an ultrafiltration enzyme hydrolysis liquid; sequentially performing gel column chromatography, ion exchange resin chromatography, and high performance liquid chromatography purification on the ultrafiltration enzyme hydrolysis liquid, so as to obtain the lipid-decreasing functional peptide. The lipid-decreasing functional peptide has the beneficial effects that most of lipid forming genes can be regulated down, the stacking of lipid is decreased, the lipid-decreasing function is realized, and the lipid-decreasing functional peptide canbe developed into lipid-decreasing medicines, health-care products and food additives.

The invention discloses a preparation method of silkworm pupa protein polypeptide. The preparation method comprises the following specific steps: adding a mixed solution of n-hexane and petroleum ether into silkworm pupa meal and carrying out heating reflux to obtain silkworm pupa protein; dissolving a silkworm pupa protein enzymatic hydrolysate into double distilled water, centrifuging to obtaina supernatant, namely silkworm pupa protein polypeptide raw liquor; adding distilled water into the silkworm pupa meal, uniformly mixing and adjusting a pH value; then adding alkaline protease and neutral protease, carrying out enzymolysis, enzyme deactivation and centrifuging, and concentrating the supernatant, namely the silkworm pupa protein polypeptide raw liquor; carrying out separation and purification on the silkworm pupa protein polypeptide raw liquor through ion-exchange resin chromatographic column, gel column chromatography and high performance liquid chromatography, thus obtainingthe silkworm pupa protein polypeptide. The preparation method of the silkworm pupa protein polypeptide, disclosed by the invention, has the beneficial effects of simplicity and feasibility; the protein polypeptide has the advantages of high yield, low loss, high purity and no possibility of destroying the activity; the obtained protein polypeptide has good inhibitory activity to ACE.

The invention relates to a production method and an application of an immune globulin G. Specifically, the production method comprises steps of: carrying out whole blood anticoagulating, centrifugal separation, diluting, FeCl3 precipitation reaction, clarifying, centrifugal separation, nano filtration and desalination, embedding, spray-drying, etc. The production method disclosed by the invention not only solves the problems of resource waste, environmental pollution and the like of animal byproduct pig blood, but also greatly improves the utilization value of the pig blood through continuous mass production processing process; the prepared immune globulin G, through an enzyme-linked immuno sorbent assay (ELISA) activity identification, is greater than 90% in activity, and has a special treating effect on intestinal diseases of small animals; and the immune globulin G can be used as an immuno potentiator for a weak animal group and as normal nutrition supplementary for the body.

The invention discloses a preparation method of silkworm chrysalis collagen. The preparation method comprises the following concrete steps: adding a degreasing agent into silkworm chrysalis meal for degreasing, carrying out suction filtration, and drying filter residues; adding a phosphate buffer solution and neutral protease into the degreased silkworm chrysalis, carrying out enzymolysis and enzyme deactivation, and cooling and then centrifuging to obtain supernatant, i.e., crude protein liquid; adding a chitosan solution into the crude protein liquid, stirring, centrifuging supernatant and putting into an ice-water bath, adding ammonium sulfate, stirring until complete dissolution, standing overnight, centrifuging, dissolving precipitate in the phosphate buffer, carrying out dialysis, enabling dialyzate to enter a hydroxyapatite column body, adding a buffer solution for eluting, and freeze-drying to obtain the silkworm chrysalis collagen. The preparation method of silkworm chrysaliscollagen is simple and feasible, and high in degreasing efficiency; the collagen is high in yield, purity and activity, and has more concentrated molecular weight; the method is suitable for industrial production and higher in safety, and increases the additional value of the silkworm chrysalis.

The invention discloses special pine-nut-protein-peptide-enriched antioxidant lozenges for astronauts and a preparation method thereof. The special pine-nut-protein-peptide-enriched antioxidant lozenges for the astronauts are prepared from the following raw materials and auxiliary materials: 20-40% of pine nut protein peptides, 10-20% of blueberry anthocyanin dry-powder, 3-6% of raspberry powder,1-2% of beta-carotene, 2-4% of taurine, 1-2% of vitamin C, 1-2% of vitamin E, 1-2% of alpha-lipoic acid, 8-15% of beta-cyclodextrin, 2-5% of buckwheat starch, 10-15% of xylitol, 2-4% of citric acid, 5-10% of fructo-oligosaccharides, and 2-4% parts of grape powder. The preparation method of the special pine-nut-protein-peptide-enriched antioxidant lozenges for the astronauts is characterized in that the pine nuts with high protein content are taken as raw materials, and the raw materials and the auxiliary materials are proportioned so as to ensure balanced nutrition; and thus, the prepared pine-nut-protein-peptide-enriched antioxidant lozenges have the characteristics of resistance to high-energy particle radiation in the space, small volume, and good taste. The pine-nut-protein-peptide-enriched antioxidant lozenges can be used for activities of astronauts in space stations, aviation pilots, field forces, navigation, submarines and so on. Moreover, the pine-nut-protein-peptide-enrichedantioxidant lozenges can be also used for people with the demands of preventing cardiovascular and cerebrovascular diseases as well as diabetes syndromes, and improving intestinal flora, reducing blood lipid and lowering blood pressure.

The invention belongs to the technical field of preparation of feed additives and particularly relates to a preparation method and application of a compound Chinese herbal medicine preparation for sheep. The preparation method comprises the steps as follows: 100-150 g of high-quality radix astragali, 60-120 g of medicated leaven, 60-120 g of malt, 60-120 g of haws, 60-120 g of dried orange peel, 60-120 g of leaves of eucommia ulmoides, 100-150 g of officinal magnolia bark, 100-200 g of houttuynia cordata, 40-80 g of garlic, 40-80 g of ginger and 60-120 g of tarragon are thoroughly cleaned anddried, the obtained materials are crushed into 60-mesh powder, ultrasonic extraction is performed twice in the ratios of medicines to water (by weight) being 1:30 and 1:20 respectively at 60 DEG C atultrasonic power of 1250 W for 60 min, extraction liquids are mixed and concentrated into extract of 4.25-5.55 (25 DEG C), the extract is mixed with calcium lactate in the ratio being 1:5, and drying,crushing, sieving and uniform mixing are performed to prepare the preparation.

The invention discloses a small molecular weight silkworm pupa collagen with a molecular weight of 600-1000Da. A preparation method of the collagen is as follows: adding a degreasing agent to silkwormpupa powder for degreasing, filtering by suction, and drying filter residue; adding a phosphate buffer liquid and neutral protease to the degreased silkworm pupa for enzymatic hydrolysis, performingenzyme deactivation, and centrifuging after cooling to obtain supernatant, namely a crude protein solution; adding a chitosan solution to the crude protein solution, stirring, centrifuging, putting supernatant into an ice water bath, adding ammonium sulfate, stirring until the ammonium sulfate is completely dissolved, standing overnight, centrifuging, dissolving a precipitate in a phosphate buffer, dialyzing, allowing dialysate into a hydroxyapatite column, adding the buffer to elute, and freezing-drying to obtain the small molecular weight silkworm pupa collagen. The beneficial effects are asfollows: the small molecular weight silkworm pupa collagen has high purity, high activity, and relatively-concentrated molecular weight, and the preparation method is simple and feasible, high in degreasing efficiency, high in yield, easy in industrial production and higher in safety and improves the added value of silkworm pupa.

The invention belongs to the technical field of preparation of traditional Chinese medicine preparations for livestock, in particular discloses a method for preparing a compound radix codonopsis preparation composition for sheep, and further relates to application of the preparation composition in a compound feed for sheep and application in an additive premixed feed for sheep. The method disclosed by the invention comprises the following steps: selecting 100-200g of high-quality radix codonopsis, 80-150g of rhizoma atractylodis macrocephalae, 50-100g of rhizoma chuanxiong, 50-100g of fructusligustri lucidi, 50-100g of fructus psoraleae, 50-100g of radix et rhizoma rhei, 50-100g of fructus cnidii, 50-100g of licorice roots, 50-100g of rhizoma atractylodis and 50-100g of seaweeds, performing cleaning, performing drying, crushing the components into powder of 60 meshes, performing ultrasonic extraction on the powder for 70 minutes by using 65% ethanol with an amount being 30 times of the amount of the powder at ultrasonic power of 900W at an ultrasonic temperature of 50 DEG C, further performing ultrasonic extraction on medicine dregs for 60 minutes by using water with an amount being 10 times of the amount of the medicine dregs at ultrasonic power of 1200W at an ultrasonic temperature of 60 DEG C, performing concentration so as to obtain an extract of which the relative densityis 3.55-4.55 at 25 DEG C, uniformly mixing the extract with hippophae rhamnoides fruit dreg powder in a ratio of 1:10, performing pelletizing, and performing drying, so as to obtain the compound radix codonopsis preparation composition.

The invention discloses an extraction method and application of peony leaf rachis crude polysaccharide. The extraction method of the peony leaf rachis crude polysaccharide comprises the following steps: step 1): pre-treating raw materials; step 2): carrying out extraction and separation on leaf rachis powder obtained by step 1) and collecting separated supernatant to obtain the peony leaf rachis crude polysaccharide, wherein the extraction and separation step is carried out according to the following manner: uniformly mixing the leaf rachis powder obtained by step 1) and an extraction agent according to the liquid/material ratio of (8 to 28)ml to 1g; extracting for 10 to 60min by utilizing an ultrasonic extractor under the conditions that the temperature is 20 to 70 DEG C and the ultrasonic power is 100 to 300W; filtering to obtain filtering dreg and a water extraction solution; centrifuging the water extraction solution and taking supernatant, wherein the supernatant is a peony leaf rachis crude polysaccharide solution. According to the extraction method disclosed by the invention, the raw materials are selected from peony leaf rachises which are originally discarded as wastes, and plant resources are sufficiently utilized; the extraction method has the advantages that an operation method flow is simple and easy to realize and the extraction efficiency is relatively high.