Cryoprotectant and lyophilization method for latex coupled antibody
A technology of latex-conjugated antibody and freeze-drying protective agent, which is applied in the field of medicine, can solve the problems of poor aggregation state and less modified latex antibody, and achieve good freeze-drying protection effect
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Embodiment 1~6
[0047]For the C-reactive protein latex antibody coupling solution, this time, a rabbit anti-human C-reactive protein latex antibody solution with a mass fraction of 0.2% was used. Add the lyoprotectant according to the scheme in Table 1 and implement the lyophilization scheme:
[0048] Table 1 Examples 1-6
[0049]
[0050] Wherein, freeze-drying procedure 1 is:
[0051]
[0052] Freeze-drying procedure 2 is:
[0053]
[0054] Freeze-drying procedure 3 is:
[0055]
[0056] Freeze-drying procedure 4 is:
[0057]
[0058] Each example was repeated 5 times, and the reconstituted appearance and antibody titer of the freeze-dried samples were tested, and the blank value was identified by immunoturbidimetry; meanwhile, the repeatability among the repetitions of each example was calculated.
[0059] Immunoturbidimetric blank value detection method: after the lyophilized product is reconstituted with distilled water, according to the National Competition-specific p...
Embodiment 7~12
[0070] A 0.4% mouse anti-human D-dimer latex antibody solution was used. Add the lyoprotectant according to the scheme in Table 2 and implement the lyophilization scheme, and the drying control procedures 1 to 4 are the same as those in Examples 1 to 6:
[0071] Table 3 Examples 7-12
[0072]
[0073] Each example was repeated 5 times, and the reconstituted appearance and antibody titer of the freeze-dried samples were tested, and the blank value was identified by immunoturbidimetry; meanwhile, the repeatability among the repetitions of each example was calculated.
[0074] The detection method of immunoturbidimetric blank value, antibody titer detection method and freeze-dried product repeatability detection method are also the same as those described in Examples 1-6.
[0075] The results are shown in Table 4:
[0076] Table 4 Test results
[0077]
[0078] As in Table 4: In terms of blank values:
[0079] Paired analysis of the detection blank value of the control ...
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