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Extraction method and application of peony leaf rachis crude polysaccharide

An extraction method and technology of peony leaves, applied in the field of extraction of crude polysaccharides from tree peony leaves, can solve the problems of low water content, neglect, waste of resources, etc.

Active Publication Date: 2018-12-18
HENAN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] For the utilization of peony plants, in addition to its root bark (moutan bark) being an important traditional Chinese medicine, peony seeds are often used to make peony seed oil or peony essential oil, and the rest of peony plants, such as peony roots, stems, leaves, and petals Most of them are directly discarded as waste; the direct discarding of a large number of peony roots, stems, leaves, and flowers every year is undoubtedly a waste of resources; It is the main shaft of the compound leaf, and the petiole grows on the rachis. Compared with petals, leaves and petioles, the rachis is easier to store for a long time because it is harder and has a lower water content.
[0003] Polysaccharide is an important biological component that can maintain the normal operation of human life activities. Plant polysaccharides have the advantages of immune regulation, anti-tumor, anti-aging, hypoglycemic and other biological activities. There are: hot water extraction method, acid extraction method, alkali extraction method, enzymatic method and ultrasonic-assisted extraction technology; the extraction process of plant polysaccharides mainly includes two physical phenomena: one is the increase of the permeability of the cell membrane, and the polysaccharides from the cell membrane The second is that the polysaccharides flow out from the cells after the cell membrane is ruptured; the most commonly used methods in the extraction of plant polysaccharides are hot water extraction and the extended water extraction and alcohol precipitation method, which concentrates the extract extracted from water Finally, add ethanol to the concentrated solution to make the final volume fraction reach about 70%. Using the insoluble properties of polysaccharides in ethanol, the polysaccharides are precipitated from the extract, and left at room temperature for several hours. The mass fraction and yield of polysaccharides are equal. Higher; however, the utilization of crude polysaccharides in peony petioles is still a technical blank

Method used

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  • Extraction method and application of peony leaf rachis crude polysaccharide

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Embodiment 1

[0050] A method for extracting crude polysaccharides from peony leaf rachis of the present invention comprises the following steps:

[0051] Step 1): Raw material pretreatment, taking peony leaf rachis with uniform thickness, removing the petiole on the peony leaf rachis, washing and drying, crushing with a pulverizer until completely passing through a 50-100 mesh sieve, and obtaining leaf rachis powder; the raw rachis is preferably Seven-year-old, vigorous growth, no pests and diseases, about 35cm long, uniform leaf rachis, remove the petiole on the leaf rachis, clean the peony leaf rachis, put them in a drying oven, dry at 60°C until constant weight, and use a cyclone Grind it and sieve it; the raw material is sufficiently crushed, which is beneficial to the dissolution of the active ingredients in the leaf axis;

[0052] Step 2): extracting and separating the rachis powder obtained in step 1), and collecting the separated supernatant to obtain the crude extract of peony rac...

Embodiment 2

[0055] A method for extracting crude polysaccharides from peony leaf rachis of the present invention comprises the following steps:

[0056] Step 1): Raw material pretreatment, taking peony leaf rachis with uniform thickness, removing the petiole on the peony leaf rachis, washing and drying, crushing with a pulverizer until completely passing through a 50-100 mesh sieve, and obtaining leaf rachis powder; the raw rachis is preferably Seven-year-old, vigorous growth, no pests and diseases, about 35cm long, uniform leaf rachis, remove the petiole on the leaf rachis, clean the peony leaf rachis, put them in a drying oven, dry at 60°C until constant weight, and use a cyclone Grind it and sieve it; the raw material is sufficiently crushed, which is beneficial to the dissolution of the active ingredients in the leaf axis;

[0057] Step 2): extracting and separating the rachis powder obtained in step 1), and collecting the separated supernatant to obtain the crude extract of peony rac...

Embodiment 3

[0060] A method for extracting crude polysaccharides from peony leaf rachis of the present invention comprises the following steps:

[0061] Step 1): Raw material pretreatment, taking peony leaf rachis with uniform thickness, removing the petiole on the peony leaf rachis, washing and drying, crushing with a pulverizer until completely passing through a 50-100 mesh sieve, and obtaining leaf rachis powder; the raw rachis is preferably Seven-year-old, vigorous growth, no pests and diseases, about 35cm long, uniform leaf rachis, remove the petiole on the leaf rachis, clean the peony leaf rachis, put them in a drying oven, dry at 60°C until constant weight, and use a cyclone Grind it and sieve it; the raw material is sufficiently crushed, which is beneficial to the dissolution of the active ingredients in the leaf axis;

[0062] Step 2): extracting and separating the rachis powder obtained in step 1), and collecting the separated supernatant to obtain the crude extract of peony rac...

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Abstract

The invention discloses an extraction method and application of peony leaf rachis crude polysaccharide. The extraction method of the peony leaf rachis crude polysaccharide comprises the following steps: step 1): pre-treating raw materials; step 2): carrying out extraction and separation on leaf rachis powder obtained by step 1) and collecting separated supernatant to obtain the peony leaf rachis crude polysaccharide, wherein the extraction and separation step is carried out according to the following manner: uniformly mixing the leaf rachis powder obtained by step 1) and an extraction agent according to the liquid / material ratio of (8 to 28)ml to 1g; extracting for 10 to 60min by utilizing an ultrasonic extractor under the conditions that the temperature is 20 to 70 DEG C and the ultrasonic power is 100 to 300W; filtering to obtain filtering dreg and a water extraction solution; centrifuging the water extraction solution and taking supernatant, wherein the supernatant is a peony leaf rachis crude polysaccharide solution. According to the extraction method disclosed by the invention, the raw materials are selected from peony leaf rachises which are originally discarded as wastes, and plant resources are sufficiently utilized; the extraction method has the advantages that an operation method flow is simple and easy to realize and the extraction efficiency is relatively high.

Description

technical field [0001] The invention relates to the extraction and application of plants, in particular to a method for extracting crude polysaccharides from peony leaf rachis and its application. Background technique [0002] For the utilization of peony plants, in addition to its root bark (moutan bark) being an important traditional Chinese medicine, peony seeds are often used to make peony seed oil or peony essential oil, and the rest of peony plants, such as peony roots, stems, leaves, and petals Most of them are directly discarded as waste; the direct discarding of a large number of peony roots, stems, leaves, and flowers every year is undoubtedly a waste of resources; It is the main axis of the compound leaf, and the petiole grows on the rachis. Compared with the petals, leaves and petioles, the rachis is more convenient for long-term preservation because it is harder and has a lower water content. [0003] Polysaccharide is an important biological component that can...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P39/06A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P39/06C08B37/0003A23V2200/30
Inventor 马晓陈刚胡继光张庆瑞胡春瑞
Owner HENAN POLYTECHNIC
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