Anti-wrinkle essence containing adipose-derived stem cell extract and preparation method of anti-wrinkle essence
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A technology of adipose stem cells and essence, which is applied in the field of anti-wrinkle essence containing adipose stem cell extract and its preparation, which can solve the problems of less cultured secretion, limited application of adipose stem cell exosomes, and high processing price
Pending Publication Date: 2020-10-27
辽宁盛京干细胞科技有限公司 +2
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Problems solved by technology
Existing isolated adipose stem cell exosomes are not of high purity, and the secretion of adipose stem cell exosomes in vitro culture is low. The
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Embodiment 1
[0030] Example 1 Preparation method of adipose stem cell exosome suspension and adipose stem cell cell content.
[0031] 1. Extraction of fat stem cells.
[0032] Rinse the adipose tissue with normal saline, repeat 2-3 times, and remove blood stains. Add enzyme solution equal to the volume of adipose tissue, digest at 37°C for 30-40min, and add 2m culture medium into the digested storage bottle. Filter the digested tissue fluid through a 100-mesh sieve, collect the digested fluid into a centrifuge tube, and centrifuge. After centrifugation, remove the supernatant and resuspend the centrifuged cell pellet. According to 50000 pieces / cm 2 The cell density was inoculated in a T75 culture flask, 15mL of culture medium was added to the culture flask, and 5% CO 2 , cultured in a 37°C incubator. Change the medium after 24 hours, and change the medium every 4 days or so. When the cell confluence reaches 70%-80%, it can be passaged or frozen.
[0033] 2. Draw the growth curve.
...
Embodiment 2
[0044] Example 2 An anti-wrinkle essence containing adipose stem cell extract and its preparation method.
[0045] An anti-wrinkle essence containing fat stem cell extract, which is composed of the following components by weight: 8 parts of fat stem cell exosome suspension, 8 parts of fat stem cell cell content, 0.5 part of mung bean peptide, and tilapia polypeptide 0.5 parts, 0.5 parts of scorpion venom polypeptide, 1.5 parts of glycerin, 1.5 parts of ethylene glycol, 0.25 parts of xanthan gum, 0.15 parts of hyaluronic acid, 1 part of phenoxyethanol, 0.03 parts of ethylhexyl, 0.1 parts of EDTA, polysorbate 0.3 part of alcohol ester, 1 part of oxidized lecithin, 0.03 part of tocopherol, 75.67 parts of distilled water.
[0046] The preparation method of the anti-wrinkle essence containing adipose stem cell extract specifically includes the following steps.
[0047] Step 1, take formula composition by weight fraction.
[0048] Step 2. Put polysorbate, oxidized lecithin, and to...
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Abstract
The invention relates to the technical field of cosmetics and biological medicines, in particular to anti-wrinkle essence containing adipose-derived stem cell extract and a preparation method of the anti-wrinkle essence. The anti-wrinkle essence containing the adipose-derived stem cell extract is composed of the following components in parts by weight: 8 to 20 parts of an adipose-derived stem cellexosome suspension, 8 to 20 parts of an adipose-derived stem cell inclusion, 0.5 to 2 parts of mung bean peptide, 0.5 to 2 parts of tilapia mossambica polypeptide, 0.5 to 2 parts of scorpion venom polypeptide, 1 to 6 parts of glycerinum, 0.1 to 4 parts of ethylene glycol, 0.1 to 4 parts of xanthan gum, 0.1 to 4 parts of hyaluronic acid, 1 to 2 parts of phenoxyethanol, 0.03 to 0.05 part of ethylhexyl, 0.1 to 0.2 part of EDTA, 0.3 to 0.8 part of polysorbate, 1 to 2 parts of oxidized lecithin, 1 to 5 parts of tocopherol and 55.2 to 94.92 parts of distilled water. The anti-wrinkle essence provided by the invention contains the adipose-derived stem cell extract, and a utility evaluation experiment proves that the anti-wrinkle essence has a certain practical effect on skin wrinkle resistance. After the anti-wrinkle essence is used, the improvement conditions of skin elasticity and wrinkle depth of volunteers are superior to those of comparative examples and commercially available anti-wrinkle products, and the anti-wrinkle essence has an obvious synergistic anti-wrinkle effect.
Description
technical field [0001] The invention relates to the technical fields of cosmetics and biomedicine, in particular to an anti-wrinkle essence containing adipose stem cell extract and a preparation method thereof. Background technique [0002] Aging is an inescapable reality. The main reasons are photochemical damage and aging changes. Chronic sunlight exposure in daily life causes abnormal arrangement and accumulation of elastic fibers in the skin; the number of collagen fibers decreases, and the remaining fibers are arranged in disordered order; abnormal border zone or marginal zone is formed between the dermis and epidermis. These changes cause fine wrinkles, sagging skin, hyperpigmentation, and skin malignancies. On the other hand, skin changes as we age. Thinning of the dermis; reduction of fibroblasts, mast cells, elastic fibers, and blood vessels. The power generated by the subcutaneous muscle contraction causes skin wrinkles, the reduction and relaxation of facial f...
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