Anti-wrinkle essence containing adipose-derived stem cell extract and preparation method of anti-wrinkle essence
A technology of adipose stem cells and essence, which is applied in the field of anti-wrinkle essence containing adipose stem cell extract and its preparation, which can solve the problems of less cultured secretion, limited application of adipose stem cell exosomes, and high processing price
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Embodiment 1
[0030] Example 1 Preparation method of adipose stem cell exosome suspension and adipose stem cell cell content.
[0031] 1. Extraction of fat stem cells.
[0032] Rinse the adipose tissue with normal saline, repeat 2-3 times, and remove blood stains. Add enzyme solution equal to the volume of adipose tissue, digest at 37°C for 30-40min, and add 2m culture medium into the digested storage bottle. Filter the digested tissue fluid through a 100-mesh sieve, collect the digested fluid into a centrifuge tube, and centrifuge. After centrifugation, remove the supernatant and resuspend the centrifuged cell pellet. According to 50000 pieces / cm 2 The cell density was inoculated in a T75 culture flask, 15mL of culture medium was added to the culture flask, and 5% CO 2 , cultured in a 37°C incubator. Change the medium after 24 hours, and change the medium every 4 days or so. When the cell confluence reaches 70%-80%, it can be passaged or frozen.
[0033] 2. Draw the growth curve.
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Embodiment 2
[0044] Example 2 An anti-wrinkle essence containing adipose stem cell extract and its preparation method.
[0045] An anti-wrinkle essence containing fat stem cell extract, which is composed of the following components by weight: 8 parts of fat stem cell exosome suspension, 8 parts of fat stem cell cell content, 0.5 part of mung bean peptide, and tilapia polypeptide 0.5 parts, 0.5 parts of scorpion venom polypeptide, 1.5 parts of glycerin, 1.5 parts of ethylene glycol, 0.25 parts of xanthan gum, 0.15 parts of hyaluronic acid, 1 part of phenoxyethanol, 0.03 parts of ethylhexyl, 0.1 parts of EDTA, polysorbate 0.3 part of alcohol ester, 1 part of oxidized lecithin, 0.03 part of tocopherol, 75.67 parts of distilled water.
[0046] The preparation method of the anti-wrinkle essence containing adipose stem cell extract specifically includes the following steps.
[0047] Step 1, take formula composition by weight fraction.
[0048] Step 2. Put polysorbate, oxidized lecithin, and to...
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