30 results about "Disease factors" patented technology

The invention discloses a high-sensitivity enzyme-linked immunoassay method. The method comprises the following steps of: according to a characteristic of specific binding of a measured object and an antibody (antigen), marking the antigen or the antibody and the corresponding antibody or antigen by a corresponding enzyme under a specific condition so as to achieve an absorption combination process, and indirectly representing the concentration of the measured object by the catalytic speed of the marked enzyme about a substrate. According to the high-sensitivity enzyme-linked immunoassay method provided by the invention, a problem of insufficient sensitivity of a regular enzyme-linked immunoassay based on a spectrophotometry is effectively overcome, and the high-sensitivity chemical oscillation dynamics detection and enzymatic dynamics analysis with specificity and flexibility amplification action are combined by utilizing an antibody-antigen specificity combination principle, so that the problems of low chemical oscillation selectivity and incapability of carrying out qualitative determination are overcome, thus the chemical oscillation in combination with the enzyme-linked immunoassay method can be used for qualitative and quantitative determination of substances such as medicine and pesticide micromolecules, antibody, antigen, specific protein, nucleic acid, disease factors and the like.

The invention relates to two codominance CAPs molecular markers which are positioned at two sides of muskmelon anti-powdery mildew gene Pm-AN and tightly linked with Pm-AN, and its application method, which belongs to the biology technical field. The markers are used for muskmelon anti-powdery mildew molecular markers for auxiliary breeding, and breeding purpose and breeding efficiency of the muskmelon anti-powdery mildew breeding can be substantially enhanced. The application of the markers is not influenced by disease factors in fields, can effectively select pure disease resistance gene type and heterozygosis disease resistance gene type individuals, and the recombinant genotype individuals are generated at two sides of the anti-powdery mildew gene. The markers can be used for disease resistance molecule polymerization breeding without transgene security problem.

The invention discloses a cultivation method of Gerbera detoxified seedlings. The cultivation method includes steps of receptacle explant pretreatment of Gerbera, inoculation and induced culture, transfer induced culture, seedling domestication and seedling hardening. By using the cultivation method, interference caused by virus and disease factors during cultivation is avoided, forming number and efficiency of callus are increased, culture period is short, and seedling qualification rate is increased; the cultivation method has wide application prospect in the field of Gerbera tissue culture.

The invention discloses a high-sensitivity enzyme-linked immunoassay method. The method comprises the following steps of: according to a characteristic of specific binding of a measured object and an antibody (antigen), marking the antigen or the antibody and the corresponding antibody or antigen by a corresponding enzyme under a specific condition so as to achieve an absorption combination process, and indirectly representing the concentration of the measured object by the catalytic speed of the marked enzyme about a substrate. According to the high-sensitivity enzyme-linked immunoassay method provided by the invention, a problem of insufficient sensitivity of a regular enzyme-linked immunoassay based on a spectrophotometry is effectively overcome, and the high-sensitivity chemical oscillation dynamics detection and enzymatic dynamics analysis with specificity and flexibility amplification action are combined by utilizing an antibody-antigen specificity combination principle, so that the problems of low chemical oscillation selectivity and incapability of carrying out qualitative determination are overcome, thus the chemical oscillation in combination with the enzyme-linked immunoassay method can be used for qualitative and quantitative determination of substances such as medicine and pesticide micromolecules, antibody, antigen, specific protein, nucleic acid, disease factors and the like.

The invention discloses dendrobium candidum compound chewable tablets which are characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of dendrobium candidum extract, 5-10 parts of lucid ganoderma, 5-15 parts of fructus lycii, 20-30 parts of sweetening agent, 3-5 parts of flavoring agent, 20-30 parts of hydroxypropyl methyl cellulose, 5-15 parts of maltodextrin and 1-3 parts of lubricant. The invention also discloses a preparation method of the chewable tablets. After the dendrobium candidum compound chewable tablets disclosed by the invention are taken, the functional balance of the internal organs of a human body can be adjusted, the immunity of the human body is improved, the immune state of the human body is effectively and bidirectionally adjusted, and the internal organs are strong and vigorous to prevent disease factors and harmful germs from invading the human body.

Health care wine is prepared from the dried mulberries, the donkey-hide gelatin, the glutinous rice and the distiller's yeast, and can effectively expand the blood volume of a human body, promote the hematopoietic function, treat blood diseases, expel toxins, relax the bowels, reduce viridin and gradually recover an immune antibody system. The health care wine also has the effects of enhancing body resistance, enhancing physique, enhancing essence and qi, promoting transformation of essence and qi, strengthening heart, tonifying lung, strengthening tendons and bones, enhancing functions of liver, kidney and spleen, enhancing digestion function, beautifying skin, keeping young, promoting melanin of hair, relieving discomfort in the menstrual period of women, repairing and adjusting body functions, and enhancing resistance of various disease factors and improving immunity of the human body; besides, the regeneration and repair of viscera tissues are promoted, internal secretion and metabolism are adjusted, appetite is promoted, sleep is improved, self-dormancy of viscera is promoted, and enhanced health-care and medical effects on a human body are achieved.

The invention discloses a construction method, system and application for a disease data analysis and processing model. The method comprises the steps of collecting disease original data and unifyinga data format; preprocessing the collected disease original data to form a large-capacity structured sample matrix; using the large-capacity structured sample matrix as data, and screening out an attribute set needed for model training to form a training sample matrix; using the training sample matrix as data, and constructing and training a best network model for disease prediction based on a feature data training method and a different error back propagation algorithm; evaluating model construction of non-unbalanced data and unbalanced data based on the correct rate and the F value; constructing a data validation mode to determine the validity of user data. According to the construction method, an auxiliary support is provided for medical diagnosis by excavating factors with the influence on disease and constructing an auxiliary diagnosis model.

The present invention relates to a novel biological detection method for dioxins in serum and a diagnostic use thereof in metabolic syndrome and related conditions. More particularly, according to the present invention, a significant correlation between dioxin content in serum and physical variables has been confirmed by measuring luciferase activity through the use of serum as a whole and cells transformed with a recombinant vector comprising a gene construct in which a dioxin responsive element, a promoter and a reporter gene are operably linked. And there is an improvement in this method of the present invention over the conventional methods which require a preprocessing step in order to purify dioxins from serum, by which plural samples can be analyzed easily and accurately even with a very small amount of each because of the use of serum as a whole. Therefore, the detection system of the present invention which involves total serum processing in a genetically transformed cell line comprising a recombinant reporter gene whose expression is modified by dioxin compounds can be effectively used for the biological detection of dioxins in serum. This method can also be used to research the correlation between specific POPs and patient's disease factors by accurate detection of POPs such as dioxins in the serum, and further this method can be effectively used to predict the occurrence of disease and to determine treatability.

The invention discloses application of recombinant human Rho kinase to preparation of medicaments. Recombinant human Rho-associated coiled-coil forming protein kinase (ROCK)1 fragment protein (RP1 for short) has the activity of the Rho kinase, so that corresponding substrates can be phosphorylated; the RP1 has the effect of contracting blood vessels, and the functional regulation effect of the blood vessels is related to the endothelium of the blood vessels, is blocked by a calcium channel blocking agent and is inhibited by Fasudil serving as an Rho kinase inhibitor; after entering bodies of animals in an injection mode, the RP1 can rise the blood pressure of the animals and has the characteristic of quick response; and the RP1 has the effect of accelerating the heart rate of the animals under the condition of providing high dose in the injection mode, and has the effect of improving the blood vessel function reduced due to various factors including disease factors. The RP1 can be applied to the preparation of medicaments for relieving, preventing and / or treating cardiovascular and cerebrovascular diseases or symptoms which are caused by various factors including disease factors and are related to the reduction of the blood vessel function.

The invention discloses an improved cloud model based on an entropy algorithm and a fault tree risk analysis method for ancient buildings, including the following steps: (1) collecting structural information of old wooden structures and establishing a fault tree, and the lowest layer of the fault tree is the disease of the basic unit (2) The disease factors are qualitatively evaluated by n experts, and the qualitative evaluation of each expert is converted into an evaluation cloud, and then the n evaluation clouds are synthesized into a disease factor cloud through an entropy-improved comprehensive cloud algorithm; (3) The disease factor cloud is synthesized upward according to the fault tree through the weighted comprehensive cloud model algorithm; (4) The risk level of the old wooden structure building is determined according to the expected value of the evaluation unit cloud at the top of the fault tree. The invention introduces the cloud model to quantitatively describe the safety evaluation data of old wooden structures, combines the advantages of the fault tree to describe the risk relationship and the fault system architecture, calculates the risk levels of each basic cause unit that may cause a fault and the overall structural unit, and provides risk ranking .

The invention discloses a disease factor data processing method. The method comprises the steps that 1, patient disease factor data is converted into a disease factor matrix through normalization; 2, the patient disease factor data is divided into different disease factor sets, and different disease factor set matrixes are obtained from the matrix; 3, for the different disease factor set matrixes, a correlative coefficient among the disease factor sets is acquired through canonical correlation analysis, and correlative factor subsets among the disease factor sets are obtained; 4, significance computation is performed by using the correlation between the disease factor matrix and the obtained disease factor sets and the obtained correlative factor subsets to obtain the significance between every two disease factor set matrixes in the different disease factor set matrixes; 5, correlative factor sets and correlative factors among the factor sets are obtained through the significance and the correlative coefficient.

The present invention relates to a composition for preventing and treating gastrointestinal diseases by using Litsea japonica, and specifically, to a composition for preventing and treating gastrointestinal diseases, containing essential oils extracted from Litsea japonica fruit as an active ingredient. According to the present invention, the essential oils extracted from Litsea japonica fruits (pure fruit, seed fruit, and seeds) and used as the active ingredient of the composition for preventing and treating gastrointestinal diseases have been confirmed to have excellent effects in an efficacy evaluation for relieving gastrointestinal diseases caused by Helicobacter pylori, which is a gastrointestinal disease factor, physical factors induced by stress, and chemical factors induced by indomethacin. Therefore, the essential oils extracted from Litsea japonica fruit (pure fruit, seed fruit, and seeds) are useful for preventing and treating gastrointestinal diseases caused by various factors.

A method and evaluation kit are provided, in which a high-capacity urate transporter is identified to assist in the early treatment and prevention of urate transport-related disease and inflammation-related disease. The method can include a step for detecting variations in genes that encode ABCG2 protein. When a subject has an SNP of V12M, R113X, Q126X, Q141K, F208S, G268R, E334X, S441N, L447V, S486N, F506SfsX4, R575X, and / or C608X, it can be concluded that the subject has a factor that is capable of inducing urate transport failure, or a state or disease attributable to that failure. When a subject has an SNP of V12M, it can be concluded that, unlike the other SNPs, there is a possibility that the subject does not possess such a factor because, although this variation itself does not lead to a change in urate transport capability, said variation is related to linkage disequilibrium with other SNPs.