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Prepn of Scorpion venom polypeptide

A technology of scorpion venom and buffer solution, which is applied in the field of preparation of scorpion venom polypeptides, can solve the problems of difficult quality requirements, unstable quality, no specific qualitative and quantitative indicators, etc.

Inactive Publication Date: 2003-05-14
赵恩成 +1
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Problems solved by technology

In the disclosed patent application documents of Chinese patent application numbers 93109133, 97120965 and 98101335, scorpion venom injections and preparation methods thereof are disclosed. The disadvantage of the preparation of scorpion venom injection is that the scorpion venom raw material in the scorpion venom injection is a mixture of scorpion venom protein, and its quality is not stable enough, which directly affects the use effect; because the raw materials of the preparation preparation do not have specific qualitative and quantitative indicators , it is difficult to meet the national requirements for the quality of pharmaceutical raw materials

Method used

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  • Prepn of Scorpion venom polypeptide
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  • Prepn of Scorpion venom polypeptide

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Embodiment Construction

[0011] The present invention is further described below:

[0012] Take CM-Sephadex G-50 chromatography column, the column length is 26×30cm. Rinse the equilibrated chromatography column with buffer solution 10 times the volume of the column, and load the sample after equilibrating the column. Dissolve 1g of scorpion venom sample (light yellow powder) in 10ml of phosphate buffer solution. After the buffer on the column bed is basically absorbed, use a dropper to gently inject the sample (loading sample) along the column wall, and dilute the remaining solution with buffer. Samples, 1-2ml each time, can be added in two times. Then the buffer solution is added to a section of liquid column in a rotating manner, covered and collected, and the flow rate is 5ml / 1200 seconds. After about 100ml of buffer is used up, change to gradient elution. Gradient elution: before the 20 tubes, it is eluted with the balance solution; after the 21 tubes, NaCl is added for gradient elution, the st...

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Abstract

The present invention relates to a biological medicine preparing method, especially, preparation process of scorpion venom polypeptide. The preparation process includes charging column balancing, elution, charging sample, gradient elution, inspection, packing and other steps. The process produces colorless or yellowish liquid scorpion venom polypeptide, which may be used in producing scorpion venom polypeptide freeze drying powder with wide application in medical field.

Description

technical field [0001] The invention relates to a biopharmaceutical method, in particular to a preparation method of a scorpion venom polypeptide. Background technique [0002] In traditional Chinese medicine, scorpion has a long history of medicinal use. In recent years, the research and application of scorpion venom has attracted more and more attention. Studies have shown that scorpion toxin is the main component of scorpion pharmacological effects, and has a strong central analgesic effect. At present, some have been used in the treatment of diseases. In the disclosed patent application documents of Chinese patent application numbers 93109133, 97120965 and 98101335, scorpion venom injections and preparation methods thereof are disclosed. The disadvantage of the preparation of scorpion venom injection is that the scorpion venom raw material in the scorpion venom injection is a mixture of scorpion venom protein, and its quality is not stable enough, which directly affect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/16C07K14/435
Inventor 孟祥军赵恩成张瑞德李娜然孟宪舒高文博顾孝先郝丕业吕洁
Owner 赵恩成
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