Detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and preparation method and application thereof

An immunochromatographic method, a technology of silver ring snake venom, applied in the field of biological detection, can solve the problems of inability to judge the species of snake bites, complex structure, and no specific rapid detection tool for rapid detection of silver ring snake bites, etc.

Active Publication Date: 2021-07-23
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

This patent uses compound antibodies with complex structures, and whether these four antibodies are highly specific to their snake venoms, one or more of the antibodies may have cross-specificity to othe

Method used

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  • Detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and preparation method and application thereof
  • Detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and preparation method and application thereof
  • Detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and preparation method and application thereof

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Embodiment Construction

[0057] 1. Preparation of β-BGT rabbit polyclonal antibody

[0058] The relatively pure Bungarotoxin (BGT) components were isolated from the crude venom of Bungalow snake, and the relatively high content and relatively specific β-bungarotoxin (β-BGT) in the crude venom was screened out as colloidal gold The candidate components of the test card were tested, and β-BGT rabbit antibody was prepared. It was found that β-BGT rabbit antibody could specifically recognize bungee venom, and it was found that β-BGT rabbit antibody was compatible with eight other domestic venomous snake venoms (cobra venom, Bamboo leaf green poison, Five-step snake venom, viper venom, pit viper venom, iron head venom at 10 mg / ml and below, king cobra venom at 2 mg / ml and below, and golden ring snake venom at 1 mg / ml and below) had no cross-reaction.

[0059] 1.1 The method for preparing rabbit anti-β-BGT polyclonal antibody by low-dose antigen rapid immunization method includes the following steps:

[00...

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Abstract

The invention belongs to the technical field of determination of biological immune methods, and particularly relates to a detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and a preparation method and application thereof. The detection card is composed of a PVC backboard, a nitrocellulose membrane (NC membrane), a colloidal gold pad, a sample pad and water absorption filter paper. The sample pad is made of glass fibers. A colloidal gold labeled rabbit anti-beta-BGT polyclonal antibody is sprayed on the colloidal gold pad. The C line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG polyclonal antibody, and the T line of the nitrocellulose membrane is coated with a rabbit anti-beta-BGT polyclonal antibody. The preparation method is simple, the specificity is high, the stability is good, the detection method is simple, carrying is convenient, bungarotoxin can be rapidly detected, whether a person is bitten by bungarus multicinctus or not can be judged within 5-10 minutes, a diagnostic tool for judging venomous snake bites can be provided for snake bite treatment, more treatment time can be gained for patients, and the detection card can assist in the clinical anti-snake venom serum dosage, and is economical and practical.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a detection card, a preparation method and an application for identifying bungee venom by colloidal gold immunochromatography. Background technique [0002] The clinical treatment of snakebite patients requires timely wound treatment and symptomatic treatment. Venomous snakebites are a global public problem, and the World Health Organization classified snakebites as a high-priority neglected tropical disease (NTD) in 2018, stating that antivenoms are the only effective antidote for snakebites. In the clinical treatment of snakebite patients, the most important thing is to accurately determine the species of the bitten snake, so that specific antivenom can be injected in a targeted manner. Different types of venomous snake bites require specific antivenom injections, because each venomous snake venom contains different components, and the lethal component...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/53C07K16/18C07K16/06C07K1/22
CPCG01N33/558G01N33/54346G01N33/5308C07K16/18C07K16/065G01N2800/42Y02A50/30
Inventor 李文辉张云陆慧娟林博
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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