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Mycoplasma pink, its solid fermentative inoculum and its application

A technology of Glioma pink and solid fermentation, which is applied in the field of its solid fermentation agent, Glioma pink, can solve the problems of chemical pollution control, complex production process, and complicated screening of bio-control bacteria, and achieve simple production process and reduced The use, beneficial effect of application and promotion

Active Publication Date: 2022-07-29
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To solve the technical problems of chemical pollution control, complex screening of biological control bacteria, and complex production process

Method used

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  • Mycoplasma pink, its solid fermentative inoculum and its application
  • Mycoplasma pink, its solid fermentative inoculum and its application
  • Mycoplasma pink, its solid fermentative inoculum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Identification of S. pink

[0044] The inventor isolated and obtained a microbial strain from the root-knot nematode, named NF-06, and its identification results were as follows:

[0045] (1) Morphological identification

[0046] The front of strain NF-06 is as figure 1 shown, the back as figure 2 As shown, the conidiophore and conidia are as image 3 shown.

[0047] It can be seen from the figure that strain NF-06 has neat colony edges on the PDA medium, and the hyphae are creeping and loose, white at the initial stage, and light pink at the later stage, and orange-red pigment is produced at the bottom of the colony. The hyphae of strain NF-06 have septa, conidiophores are born directly from the hyphae, colorless, with broom-like branches at the top, and small stalks at the ends of the meristems, which are in the shape of antorius, and the conidia are born in The top of the small stalk, oblong, with a size of 2.0~3.5 um × 5.5~6.5 um, has been morpholo...

Embodiment 2

[0051] Example 2: Detection of parasitic effect and lethal effect of NF-06 strain on second instar larvae of root knot nematodes

[0052] The FF-128 strain was isolated from the tomato root soil in the greenhouse of Luowang Township, Xiangfu District, Kaifeng City, Henan Province by the inventor (Zhang Jie) in March 2017. It has strong inhibitory effect on tomato gray mold and watermelon fusarium wilt.

[0053]In this experiment, the FF-128 strain of G. pink was used as the control, and the lethal effects and parasitic effects of the strain NF-06 and the strain FF-128 on the second instar larvae of R. incognita were determined.

[0054] The specific steps are as follows: inoculate the bacterial cake (9mm) of strain NF-06 and strain FF-128 into water agar medium, and after culturing at 25°C for 3 days, place four sterile small coverslips around the colony, and inoculate 10 μL of nematode The solution (2000 strips / mL) was added dropwise to the cover glass, the petri dish was se...

Embodiment 3

[0057] Example 3: Preparation of Solid Fermentation Inoculum of Phytophthora pink strain NF-06

[0058] (1) Preparation of seed solution of Mycoplasma pink strain NF-06

[0059] The strain NF-06 of G. pink was activated on PDA solid medium and cultured at 25°C for 5 days;

[0060] Divide the prepared PD medium into 250ml conical flasks with 50ml each, autoclave at 121°C for half an hour, and then use it for later use. On the ultra-clean workbench, use a punch along the edge of the activated colony to make diameters. The 5mm bacterial cake was inoculated onto the PD medium with an inoculating needle, and the seed liquid was obtained after culturing at 25°C and 180r / min for 48h.

[0061] (2) Screening basal medium

[0062] Using wheat bran, wheat straw and soybean meal as the raw materials for the basal medium, the basal medium was screened. As shown in Table 1, the initial water content was 40%, and the inoculation was carried out according to the inoculation amount of 3% (wt...

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Abstract

The invention discloses a mildew Mycoplasma pink, its solid fermentation inoculant and its application, and aims to solve the technical problems of chemical pollution control, complicated biocontrol bacteria screening and complicated production process. Screening a strain of Slime sclerotiorum ( Clonostachys rosea ) NF‑06, whose deposit number is CGMCC No.16262. Culturing and preparing a solid fermentation inoculum obtained by solid fermentation of Mycoplasma pink strain NF-06. The Myxoplasma pink strain NF-06 or the solid fermentative inoculum is used in the control of plant root-knot nematode disease. The method of the invention is simple and feasible, low in cost, high in effective viable bacteria count of the spore powder, friendly to ecological safety, less chemical agent is used, simple in production process, low in cost, and beneficial for application and popularization.

Description

technical field [0001] The invention relates to the technical field of agricultural biological control, in particular to a kind of S. pink, its solid fermentation inoculum and its application. Background technique [0002] Diseases and insect pests are important factors affecting the yield and quality of agricultural products, among which root-knot nematodes are increasingly harmful to crop growth. Root-knot nematodes mainly infect the roots of plants, causing root knots or galls in the root system. The roots of severely diseased plants are atrophied and deformed, and the function of absorbing nutrients is impaired, resulting in plant stunting, premature aging and other symptoms. Wounds, leading to the occurrence of fungal diseases such as fusarium wilt and root rot. After the occurrence of root-knot nematode disease, the yield is generally reduced by 10% to 20%, and can reach more than 75% in severe cases, which has become a major obstacle in agricultural production. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P5/00C12R1/645
Inventor 张洁杨丽荣郭雪萍夏明聪孙润红汤蒙蒙武超
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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