A kind of pathogenic fungi used for biological control of cockroaches

A microbial mixture and microbial agent technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of human and animal hazards, weak virulence, poor host selectivity, long killing time, etc., to achieve control of insect populations, Strong selectivity, the effect of protecting natural enemies

Active Publication Date: 2019-06-28
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is aimed at the extensive drug resistance, environmental pollution, harm to humans and animals and weak toxicity, low lethality, long insecticidal time, poor selectivity to hosts and whether it can Kill many problems such as German cockroaches in various growth stages, provide a kind of scarab metarhizium anisopliae, use this entomopathogenic fungus to prevent and control pests that are not easy to produce drug resistance, have strong selectivity to hosts, and have characteristics such as environmental safety. Development and utilization of biopesticides to achieve the purpose of pest control

Method used

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  • A kind of pathogenic fungi used for biological control of cockroaches
  • A kind of pathogenic fungi used for biological control of cockroaches
  • A kind of pathogenic fungi used for biological control of cockroaches

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 Screening of Metarhizium anisopliae EB0732 (Metarhizium anisopliae)

[0037] (1) Screening process

[0038] Separation and purification were all carried out under the ultra-clean workbench: use an inoculation needle to dip the spore powder on the surface of the German cockroach scorpion and streak it on the PDA medium with a diameter of 90mm smeared with 0.5% gentamicin, or use sterilized Pick up the worms with tweezers and shake them gently and quickly on the above-mentioned medium for a few times. Placed in a 26°C constant temperature incubator for cultivation in the dark. After 3 days, pick the culture on the culture medium and transfer it to a new plate PDA medium with a diameter of 90 mm and smeared with gentamicin, and repeat the operation several times to obtain pure strains. And use the isolated Metarhizium anisopliae pure culture to reinfect healthy German cockroaches, and re-isolate the same Metarhizium anisopliae from the corpses of German cockroa...

Embodiment 2

[0044] The virulence determination of embodiment 2 scarab metarhizium anisopliae EB0732 (Metarhizium anisopliae) to German cockroach

[0045] Take the conidia of Metarhizium anisopliae on the PDA medium, carry out the concentration gradient drop bioassay, and prepare the spores of Metarhizium anisopliae into 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 For five spore concentrations per CFU / ml, 0.5% (w / w, accounting for the mass of the spore solution) Tween 80 was added dropwise and mixed evenly for later use. Take 1-3 instar nymphs, 3-5 instar nymphs, 5-7 instar male and female nymphs, and male and female adults of German cockroach healthy individuals of the sensitive strain, 20 in each group, and use a micropipette to draw 1 μL of spore suspension of different concentrations into the The dorsal plate of the German cockroach began to die after 24 hours, and the cumulative mortality of the German cockroach was recorded until 20 days after observation. The experimental data wa...

Embodiment 3

[0059] The metarhizium anisopliae of the present invention has strong selectivity, and it can be obtained through tests that the fungus has outstanding pathogenicity to cockroaches, especially German cockroaches, and relatively low pathogenicity to other cockroaches , the effect is not significant; there is no toxicity to vertebrates (fish, etc.) and other common beneficial economic insects (such as spiders, parasitic wasps, etc.), which is conducive to the protection of natural enemies and is environmentally safe.

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Abstract

The invention discloses a pathogenic fungus used for biological control of cockroaches, and the taxonomic name of the strain is Metarhizium anisopliae ( Metarhizium anisopliae ), the number is EB0732, and the strain has been deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on October 31, 2016, and the preservation number is CGMCC No.13170. Beneficial effects: (1) The pathogenic fungus is not easy to develop resistance to pests. (2) The pathogenic fungus has strong selectivity to the host and is safe to the environment. (3) The Metarhizium anisopliae of the present invention has a strong lethal effect on male and female pests of different ages.

Description

technical field [0001] The invention specifically relates to a pathogenic fungus used for biological control of cockroaches. Background technique [0002] Cockroaches, commonly known as cockroaches, generally refer to insects belonging to the order Blattodea. According to literature records, current taxonomists divide cockroaches into about 6,000 species, of which about 50 species are pests, such as the common Asian cockroaches. In addition, the American cockroach (Periplaneta americana), German cockroach (Bladenola germanica), brown cockroach and Australian cockroach are also widely distributed. There are more than 200 species in my country, and there are about 10 common indoor cockroaches, which vary from place to place. The common ones are Blattella germanica and Periplaneta americana. German cockroach (scientific name Blattella germanica), also known as German cockroach, German cockroach, originally from Africa, is a close relative of Asian cockroach. The German cockro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 张凡张宪翠张硕卢洁武婷王敏王誉蓉夏永明
Owner SHANDONG NORMAL UNIV
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