A kind of pathogenic fungi used for biological control of cockroaches
A microbial mixture and microbial agent technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of human and animal hazards, weak virulence, poor host selectivity, long killing time, etc., to achieve control of insect populations, Strong selectivity, the effect of protecting natural enemies
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Embodiment 1
[0036] Example 1 Screening of Metarhizium anisopliae EB0732 (Metarhizium anisopliae)
[0037] (1) Screening process
[0038] Separation and purification were all carried out under the ultra-clean workbench: use an inoculation needle to dip the spore powder on the surface of the German cockroach scorpion and streak it on the PDA medium with a diameter of 90mm smeared with 0.5% gentamicin, or use sterilized Pick up the worms with tweezers and shake them gently and quickly on the above-mentioned medium for a few times. Placed in a 26°C constant temperature incubator for cultivation in the dark. After 3 days, pick the culture on the culture medium and transfer it to a new plate PDA medium with a diameter of 90 mm and smeared with gentamicin, and repeat the operation several times to obtain pure strains. And use the isolated Metarhizium anisopliae pure culture to reinfect healthy German cockroaches, and re-isolate the same Metarhizium anisopliae from the corpses of German cockroa...
Embodiment 2
[0044] The virulence determination of embodiment 2 scarab metarhizium anisopliae EB0732 (Metarhizium anisopliae) to German cockroach
[0045] Take the conidia of Metarhizium anisopliae on the PDA medium, carry out the concentration gradient drop bioassay, and prepare the spores of Metarhizium anisopliae into 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 For five spore concentrations per CFU / ml, 0.5% (w / w, accounting for the mass of the spore solution) Tween 80 was added dropwise and mixed evenly for later use. Take 1-3 instar nymphs, 3-5 instar nymphs, 5-7 instar male and female nymphs, and male and female adults of German cockroach healthy individuals of the sensitive strain, 20 in each group, and use a micropipette to draw 1 μL of spore suspension of different concentrations into the The dorsal plate of the German cockroach began to die after 24 hours, and the cumulative mortality of the German cockroach was recorded until 20 days after observation. The experimental data wa...
Embodiment 3
[0059] The metarhizium anisopliae of the present invention has strong selectivity, and it can be obtained through tests that the fungus has outstanding pathogenicity to cockroaches, especially German cockroaches, and relatively low pathogenicity to other cockroaches , the effect is not significant; there is no toxicity to vertebrates (fish, etc.) and other common beneficial economic insects (such as spiders, parasitic wasps, etc.), which is conducive to the protection of natural enemies and is environmentally safe.
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