Aspergillus awamori strain and its use in the field of agricultural pest control
A technology for Aspergillus awamori and Aspergillus awamori, which is applied to Aspergillus awamori strains and in the fields of agricultural pest control and biological pesticides, can solve the problems of destroying ecological balance, endangering grassland ecological environment, poor safety, etc., achieving strong lethal effect and being widely used Foreground effect
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Embodiment 1
[0039] Example 1 Isolation, screening and identification of Aspergillus awamori
[0040] 1. Medium
[0041] Potato dextrose agar medium (PDA): 200 g of potatoes, 20 g of anhydrous glucose, 20 g of agar powder, and 1000 ml of distilled water.
[0042] 2. Screening and purification of bacterial strains
[0043] (1) Source of bacteria
[0044] The source of bacteria was collected from 26 soil samples collected at a distance of 3 to 6 cm from the surface in Ganzi Prefecture, Sichuan Province and Dabei Mountain in Urumqi.
[0045] (2) Isolation and purification of bacterial strains
[0046] Accurately weigh 10 g of the soil sample, add it to a conical flask filled with 90 ml of sterile water and glass beads, and shake for about 20 minutes to fully mix the soil sample with the water and disperse the cells. Prepare a series of soil dilutions with concentration gradients, and then absorb 1ml each and spread the bacterial solution evenly on the plate with a sterile glass smear stic...
Embodiment 2
[0052] Embodiment 2 Preparation of Aspergillus awamori fungal fermentation broth and Aspergillus awamori metabolites
[0053] The isolated fungus was inoculated into 500 ml of sterile liquid PDA medium containing 250 ml, 165 r / min, 26 ° C shaking culture for 7 to 14 days to obtain 80 L of fungal fermentation broth.
[0054] Use filter paper to filter and centrifuge at 10,000 rpm at 4°C to obtain the supernatant of the fermentation broth. Add 95% ethanol according to the ratio of fermented liquid and solvent 1:3 (W / V), cold soak at room temperature for 24 hours, stir and extract in a water bath at 50°C for 6 hours, and then filter with suction to obtain a dark brown ethanol extract, and extract in this way for 3 times, The combined ethanol extracts were concentrated by a rotary evaporator to prepare a dark brown pasty ethanol crude extract, which is the metabolite of Aspergillus awamori.
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